Cargando…

Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection

The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using a rapid and inexpensive fabrication method which utilises colloidal dispersion enhanced using sonication. This technique was used to prepare highly ordered and uniform nano-crystalline sensor su...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Lu, Kiely, Janice, Piano, Martina, Luxton, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107546/
https://www.ncbi.nlm.nih.gov/pubmed/30140055
http://dx.doi.org/10.1038/s41598-018-30793-z
_version_ 1783349983873859584
author Cao, Lu
Kiely, Janice
Piano, Martina
Luxton, Richard
author_facet Cao, Lu
Kiely, Janice
Piano, Martina
Luxton, Richard
author_sort Cao, Lu
collection PubMed
description The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using a rapid and inexpensive fabrication method which utilises colloidal dispersion enhanced using sonication. This technique was used to prepare highly ordered and uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) using 0.5%, 1% and 5% concentrations of zinc oxide nano-crystal suspensions. Impedance spectroscopy was employed to interrogate the sensor surfaces and confirmed high reproducibility of the fabrication process. Changes in impedance values, at a frequency of 138 Hz, were used to establish dose dependent responses for C-reactive protein (CRP) antigen. A limit of detection of less than 1 ng/ml was demonstrated for nano-surfaces fabricated from concentrations of 1% ZnO.
format Online
Article
Text
id pubmed-6107546
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61075462018-08-28 Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection Cao, Lu Kiely, Janice Piano, Martina Luxton, Richard Sci Rep Article The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using a rapid and inexpensive fabrication method which utilises colloidal dispersion enhanced using sonication. This technique was used to prepare highly ordered and uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) using 0.5%, 1% and 5% concentrations of zinc oxide nano-crystal suspensions. Impedance spectroscopy was employed to interrogate the sensor surfaces and confirmed high reproducibility of the fabrication process. Changes in impedance values, at a frequency of 138 Hz, were used to establish dose dependent responses for C-reactive protein (CRP) antigen. A limit of detection of less than 1 ng/ml was demonstrated for nano-surfaces fabricated from concentrations of 1% ZnO. Nature Publishing Group UK 2018-08-23 /pmc/articles/PMC6107546/ /pubmed/30140055 http://dx.doi.org/10.1038/s41598-018-30793-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cao, Lu
Kiely, Janice
Piano, Martina
Luxton, Richard
Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
title Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
title_full Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
title_fullStr Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
title_full_unstemmed Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
title_short Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
title_sort facile and inexpensive fabrication of zinc oxide based bio-surfaces for c-reactive protein detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107546/
https://www.ncbi.nlm.nih.gov/pubmed/30140055
http://dx.doi.org/10.1038/s41598-018-30793-z
work_keys_str_mv AT caolu facileandinexpensivefabricationofzincoxidebasedbiosurfacesforcreactiveproteindetection
AT kielyjanice facileandinexpensivefabricationofzincoxidebasedbiosurfacesforcreactiveproteindetection
AT pianomartina facileandinexpensivefabricationofzincoxidebasedbiosurfacesforcreactiveproteindetection
AT luxtonrichard facileandinexpensivefabricationofzincoxidebasedbiosurfacesforcreactiveproteindetection