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High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes

This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to m...

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Autores principales: El Kheir, Sara M., Cherrat, Lamia, Awussi, Ahoefa A., Ramia, Nancy E., Taha, Samir, Rahman, Abdur, Passerini, Delphine, Leroi, Françoise, Petit, Jeremy, Mangavel, Cécile, Revol-Junelles, Anne-Marie, Borges, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107680/
https://www.ncbi.nlm.nih.gov/pubmed/30174662
http://dx.doi.org/10.3389/fmicb.2018.01883
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author El Kheir, Sara M.
Cherrat, Lamia
Awussi, Ahoefa A.
Ramia, Nancy E.
Taha, Samir
Rahman, Abdur
Passerini, Delphine
Leroi, Françoise
Petit, Jeremy
Mangavel, Cécile
Revol-Junelles, Anne-Marie
Borges, Frédéric
author_facet El Kheir, Sara M.
Cherrat, Lamia
Awussi, Ahoefa A.
Ramia, Nancy E.
Taha, Samir
Rahman, Abdur
Passerini, Delphine
Leroi, Françoise
Petit, Jeremy
Mangavel, Cécile
Revol-Junelles, Anne-Marie
Borges, Frédéric
author_sort El Kheir, Sara M.
collection PubMed
description This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for high-throughput screening (HTS) approaches. The method was applied to screen a collection of 1810 strains isolated from raw cow’s milk in order to identify non-acidifying strains with anti-L. monocytogenes bioprotection properties. This method was also successfully used to identify anti-L. monocytogenes candidates within a collection of Lactococcus piscium, a species where antagonism was previously described as non-diffusible and requiring cell-to-cell contact. In conclusion, bioluminescent L. monocytogenes can be used in HTS to identify strains with anti-L. monocytogenes bioprotection properties, irrespectively of the inhibition mechanism.
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spelling pubmed-61076802018-08-31 High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes El Kheir, Sara M. Cherrat, Lamia Awussi, Ahoefa A. Ramia, Nancy E. Taha, Samir Rahman, Abdur Passerini, Delphine Leroi, Françoise Petit, Jeremy Mangavel, Cécile Revol-Junelles, Anne-Marie Borges, Frédéric Front Microbiol Microbiology This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for high-throughput screening (HTS) approaches. The method was applied to screen a collection of 1810 strains isolated from raw cow’s milk in order to identify non-acidifying strains with anti-L. monocytogenes bioprotection properties. This method was also successfully used to identify anti-L. monocytogenes candidates within a collection of Lactococcus piscium, a species where antagonism was previously described as non-diffusible and requiring cell-to-cell contact. In conclusion, bioluminescent L. monocytogenes can be used in HTS to identify strains with anti-L. monocytogenes bioprotection properties, irrespectively of the inhibition mechanism. Frontiers Media S.A. 2018-08-17 /pmc/articles/PMC6107680/ /pubmed/30174662 http://dx.doi.org/10.3389/fmicb.2018.01883 Text en Copyright © 2018 El Kheir, Cherrat, Awussi, Ramia, Taha, Rahman, Passerini, Leroi, Petit, Mangavel, Revol-Junelles and Borges. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
El Kheir, Sara M.
Cherrat, Lamia
Awussi, Ahoefa A.
Ramia, Nancy E.
Taha, Samir
Rahman, Abdur
Passerini, Delphine
Leroi, Françoise
Petit, Jeremy
Mangavel, Cécile
Revol-Junelles, Anne-Marie
Borges, Frédéric
High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
title High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
title_full High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
title_fullStr High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
title_full_unstemmed High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
title_short High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
title_sort high-throughput identification of candidate strains for biopreservation by using bioluminescent listeria monocytogenes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107680/
https://www.ncbi.nlm.nih.gov/pubmed/30174662
http://dx.doi.org/10.3389/fmicb.2018.01883
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