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Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W

Lactobacillus casei LC2W, a probiotic strain, can produce exopolysaccharide (EPS) with anti-hypertensive bioactivity. The relationship between eps genes and EPS synthesis in LC2W due to unclear regulation mechanism of EPS biosynthesis was investigated. The several relevant genes in EPS biosynthetic...

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Autores principales: Song, Xin, Xiong, Zhiqiang, Kong, Linghui, Wang, Guangqiang, Ai, Lianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107683/
https://www.ncbi.nlm.nih.gov/pubmed/30174661
http://dx.doi.org/10.3389/fmicb.2018.01882
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author Song, Xin
Xiong, Zhiqiang
Kong, Linghui
Wang, Guangqiang
Ai, Lianzhong
author_facet Song, Xin
Xiong, Zhiqiang
Kong, Linghui
Wang, Guangqiang
Ai, Lianzhong
author_sort Song, Xin
collection PubMed
description Lactobacillus casei LC2W, a probiotic strain, can produce exopolysaccharide (EPS) with anti-hypertensive bioactivity. The relationship between eps genes and EPS synthesis in LC2W due to unclear regulation mechanism of EPS biosynthesis was investigated. The several relevant genes in EPS biosynthetic gene cluster were deleted, overexpressed and complemented. The results suggested that glucose-1-phosphate thymidyltranseferase gene (LC2W_2179), uncharacterized EPS biosynthesis protein (LC2W_2188), and EPS biosynthesis protein (LC2W_2189) were related to EPS biosynthesis. EPS titer decreased 15, 13, and 21% when the three genes were deleted, respectively. When they were overexpressed, EPS titer increased 16, 10, and 18%. When they were complemented, EPS titer was similar to the wild-type strain. This work showed the three eps genes from LC2W played important roles on EPS production.
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spelling pubmed-61076832018-08-31 Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W Song, Xin Xiong, Zhiqiang Kong, Linghui Wang, Guangqiang Ai, Lianzhong Front Microbiol Microbiology Lactobacillus casei LC2W, a probiotic strain, can produce exopolysaccharide (EPS) with anti-hypertensive bioactivity. The relationship between eps genes and EPS synthesis in LC2W due to unclear regulation mechanism of EPS biosynthesis was investigated. The several relevant genes in EPS biosynthetic gene cluster were deleted, overexpressed and complemented. The results suggested that glucose-1-phosphate thymidyltranseferase gene (LC2W_2179), uncharacterized EPS biosynthesis protein (LC2W_2188), and EPS biosynthesis protein (LC2W_2189) were related to EPS biosynthesis. EPS titer decreased 15, 13, and 21% when the three genes were deleted, respectively. When they were overexpressed, EPS titer increased 16, 10, and 18%. When they were complemented, EPS titer was similar to the wild-type strain. This work showed the three eps genes from LC2W played important roles on EPS production. Frontiers Media S.A. 2018-08-17 /pmc/articles/PMC6107683/ /pubmed/30174661 http://dx.doi.org/10.3389/fmicb.2018.01882 Text en Copyright © 2018 Song, Xiong, Kong, Wang and Ai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Song, Xin
Xiong, Zhiqiang
Kong, Linghui
Wang, Guangqiang
Ai, Lianzhong
Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W
title Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W
title_full Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W
title_fullStr Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W
title_full_unstemmed Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W
title_short Relationship Between Putative eps Genes and Production of Exopolysaccharide in Lactobacillus casei LC2W
title_sort relationship between putative eps genes and production of exopolysaccharide in lactobacillus casei lc2w
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107683/
https://www.ncbi.nlm.nih.gov/pubmed/30174661
http://dx.doi.org/10.3389/fmicb.2018.01882
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