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Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation
Background: Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun- subunits of NF-κB and AP-1 tra...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iran University of Medical Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108266/ https://www.ncbi.nlm.nih.gov/pubmed/30159261 http://dx.doi.org/10.14196/mjiri.32.10 |
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author | Mohammadpou, Zinat Amiri, Fatemehsadat Saboor-Yaraghi, Ali Akbar Koohdani, Fariba Norouzzadeh, Marjan Sharifi, Loghman SeyyedSalehi, MonirehSadat Ebrahimi, Amirpasha Mahmoudi, Maryam |
author_facet | Mohammadpou, Zinat Amiri, Fatemehsadat Saboor-Yaraghi, Ali Akbar Koohdani, Fariba Norouzzadeh, Marjan Sharifi, Loghman SeyyedSalehi, MonirehSadat Ebrahimi, Amirpasha Mahmoudi, Maryam |
author_sort | Mohammadpou, Zinat |
collection | PubMed |
description | Background: Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun- subunits of NF-κB and AP-1 transcription factors. Methods: HepG2 cells were settled in a serum- free medium with high concentrations of glucose (30 mM) and insulin (1 µM) overnight and were then incubated with RSV (5, 10, and 20 µM) for 24 and 48 hours. Real time quantitative polymerase chain reaction (qRT-PCR) was used to determine RelA and c-Jun expression. Results: RSV diminished hyperglycemia/hyperinsulinemia stimulated expression of c-Jun dose- dependently after 24 and 48 hours (p<0.05). In addition, RelA gene expression was decreased dose-dependently in all RSV doses after 48-hour incubation (p<0.05). Our results indicated that RSV may reduce NF-κB and AP-1 activity via RelA and c-Jun gene regulation. Conclusion: The findings of the present study demonstrated that RSV may be considered as a preventative and therapeutic agent for antagonizing inflammation in Hepatocellular carcinoma (HCC). |
format | Online Article Text |
id | pubmed-6108266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Iran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-61082662018-08-29 Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation Mohammadpou, Zinat Amiri, Fatemehsadat Saboor-Yaraghi, Ali Akbar Koohdani, Fariba Norouzzadeh, Marjan Sharifi, Loghman SeyyedSalehi, MonirehSadat Ebrahimi, Amirpasha Mahmoudi, Maryam Med J Islam Repub Iran Original Article Background: Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun- subunits of NF-κB and AP-1 transcription factors. Methods: HepG2 cells were settled in a serum- free medium with high concentrations of glucose (30 mM) and insulin (1 µM) overnight and were then incubated with RSV (5, 10, and 20 µM) for 24 and 48 hours. Real time quantitative polymerase chain reaction (qRT-PCR) was used to determine RelA and c-Jun expression. Results: RSV diminished hyperglycemia/hyperinsulinemia stimulated expression of c-Jun dose- dependently after 24 and 48 hours (p<0.05). In addition, RelA gene expression was decreased dose-dependently in all RSV doses after 48-hour incubation (p<0.05). Our results indicated that RSV may reduce NF-κB and AP-1 activity via RelA and c-Jun gene regulation. Conclusion: The findings of the present study demonstrated that RSV may be considered as a preventative and therapeutic agent for antagonizing inflammation in Hepatocellular carcinoma (HCC). Iran University of Medical Sciences 2018-02-10 /pmc/articles/PMC6108266/ /pubmed/30159261 http://dx.doi.org/10.14196/mjiri.32.10 Text en © 2018 Iran University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0), which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Mohammadpou, Zinat Amiri, Fatemehsadat Saboor-Yaraghi, Ali Akbar Koohdani, Fariba Norouzzadeh, Marjan Sharifi, Loghman SeyyedSalehi, MonirehSadat Ebrahimi, Amirpasha Mahmoudi, Maryam Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation |
title | Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation |
title_full | Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation |
title_fullStr | Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation |
title_full_unstemmed | Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation |
title_short | Resveratrol suppresses hyperglycemia-induced activation of NF-κB and AP-1 via c-Jun and RelA gene regulation |
title_sort | resveratrol suppresses hyperglycemia-induced activation of nf-κb and ap-1 via c-jun and rela gene regulation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108266/ https://www.ncbi.nlm.nih.gov/pubmed/30159261 http://dx.doi.org/10.14196/mjiri.32.10 |
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