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Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells

OBJECTIVES: Calcineurin B (CNB) is a regulatory subunit of calcineurin, and it has antitumor activity. In this study, we aimed to investigate the effect of recombinant human calcineurin B (rhCNB) on the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. MATERIALS AND...

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Autores principales: Guo, Yanzi, Huang, Yonghao, Tian, Shuhong, Xie, Xueli, Xing, Guilan, Fu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108332/
https://www.ncbi.nlm.nih.gov/pubmed/30154649
http://dx.doi.org/10.2147/DDDT.S171675
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author Guo, Yanzi
Huang, Yonghao
Tian, Shuhong
Xie, Xueli
Xing, Guilan
Fu, Jian
author_facet Guo, Yanzi
Huang, Yonghao
Tian, Shuhong
Xie, Xueli
Xing, Guilan
Fu, Jian
author_sort Guo, Yanzi
collection PubMed
description OBJECTIVES: Calcineurin B (CNB) is a regulatory subunit of calcineurin, and it has antitumor activity. In this study, we aimed to investigate the effect of recombinant human calcineurin B (rhCNB) on the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. MATERIALS AND METHODS: Cell viability and cell proliferation were detected by MTT and BrdU assay. Flow cytometry, Western blot and immunohistochemistry were performed to determine rhCNB-induced apoptosis and cell cycle arrest. The antitumor activities of rhCNB were observed in mice tumor models. RESULTS: We demonstrated that rhCNB inhibits the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. We showed that the inhibition of cell proliferation by rhCNB is associated with apoptosis and cell cycle arrest in both tumor cell lines. Furthermore, we indicated that rhCNB promotes p53 protein expression, a potent proapoptotic factor. Meanwhile, we also exhibited that rhCNB decreases the expression of both cyclin B1 and CDK1 proteins, two proteins associated with G(2)/M arrest. CONCLUSION: Together, these findings suggest that rhCNB markedly inhibits tumor growth and provides guidance for its drug development.
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spelling pubmed-61083322018-08-28 Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells Guo, Yanzi Huang, Yonghao Tian, Shuhong Xie, Xueli Xing, Guilan Fu, Jian Drug Des Devel Ther Original Research OBJECTIVES: Calcineurin B (CNB) is a regulatory subunit of calcineurin, and it has antitumor activity. In this study, we aimed to investigate the effect of recombinant human calcineurin B (rhCNB) on the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. MATERIALS AND METHODS: Cell viability and cell proliferation were detected by MTT and BrdU assay. Flow cytometry, Western blot and immunohistochemistry were performed to determine rhCNB-induced apoptosis and cell cycle arrest. The antitumor activities of rhCNB were observed in mice tumor models. RESULTS: We demonstrated that rhCNB inhibits the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. We showed that the inhibition of cell proliferation by rhCNB is associated with apoptosis and cell cycle arrest in both tumor cell lines. Furthermore, we indicated that rhCNB promotes p53 protein expression, a potent proapoptotic factor. Meanwhile, we also exhibited that rhCNB decreases the expression of both cyclin B1 and CDK1 proteins, two proteins associated with G(2)/M arrest. CONCLUSION: Together, these findings suggest that rhCNB markedly inhibits tumor growth and provides guidance for its drug development. Dove Medical Press 2018-08-20 /pmc/articles/PMC6108332/ /pubmed/30154649 http://dx.doi.org/10.2147/DDDT.S171675 Text en © 2018 Guo et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Guo, Yanzi
Huang, Yonghao
Tian, Shuhong
Xie, Xueli
Xing, Guilan
Fu, Jian
Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
title Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
title_full Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
title_fullStr Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
title_full_unstemmed Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
title_short Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
title_sort genetically engineered drug rhcnb induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108332/
https://www.ncbi.nlm.nih.gov/pubmed/30154649
http://dx.doi.org/10.2147/DDDT.S171675
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