Cargando…

Nanoplasmonic pillars engineered for single exosome detection

Exosomes are secreted nanovesicles which incorporate proteins and nucleic acids, thereby enabling multifunctional pathways for intercellular communication. There is an increasing appreciation of the critical role they play in fundamental processes such as development, wound healing and disease progr...

Descripción completa

Detalles Bibliográficos
Autores principales: Raghu, Deepa, Christodoulides, Joseph A., Christophersen, Marc, Liu, Jinny L., Anderson, George P., Robitaille, Michael, Byers, Jeff M., Raphael, Marc P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108516/
https://www.ncbi.nlm.nih.gov/pubmed/30142169
http://dx.doi.org/10.1371/journal.pone.0202773
_version_ 1783350158927331328
author Raghu, Deepa
Christodoulides, Joseph A.
Christophersen, Marc
Liu, Jinny L.
Anderson, George P.
Robitaille, Michael
Byers, Jeff M.
Raphael, Marc P.
author_facet Raghu, Deepa
Christodoulides, Joseph A.
Christophersen, Marc
Liu, Jinny L.
Anderson, George P.
Robitaille, Michael
Byers, Jeff M.
Raphael, Marc P.
author_sort Raghu, Deepa
collection PubMed
description Exosomes are secreted nanovesicles which incorporate proteins and nucleic acids, thereby enabling multifunctional pathways for intercellular communication. There is an increasing appreciation of the critical role they play in fundamental processes such as development, wound healing and disease progression, yet because of their heterogeneous molecular content and low concentrations in vivo, their detection and characterization remains a challenge. In this work we combine nano- and microfabrication techniques for the creation of nanosensing arrays tailored toward single exosome detection. Elliptically–shaped nanoplasmonic sensors are fabricated to accommodate at most one exosome and individually imaged in real time, enabling the label-free recording of digital responses in a highly multiplexed geometry. This approach results in a three orders of magnitude sensitivity improvement over previously reported real-time, multiplexed platforms. Each nanosensor is elevated atop a quartz nanopillar, minimizing unwanted nonspecific substrate binding contributions. The approach is validated with the detection of exosomes secreted by MCF7 breast adenocarcinoma cells. We demonstrate the increasingly digital and stochastic nature of the response as the number of subsampled nanosensors is reduced from four hundred to one.
format Online
Article
Text
id pubmed-6108516
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-61085162018-09-18 Nanoplasmonic pillars engineered for single exosome detection Raghu, Deepa Christodoulides, Joseph A. Christophersen, Marc Liu, Jinny L. Anderson, George P. Robitaille, Michael Byers, Jeff M. Raphael, Marc P. PLoS One Research Article Exosomes are secreted nanovesicles which incorporate proteins and nucleic acids, thereby enabling multifunctional pathways for intercellular communication. There is an increasing appreciation of the critical role they play in fundamental processes such as development, wound healing and disease progression, yet because of their heterogeneous molecular content and low concentrations in vivo, their detection and characterization remains a challenge. In this work we combine nano- and microfabrication techniques for the creation of nanosensing arrays tailored toward single exosome detection. Elliptically–shaped nanoplasmonic sensors are fabricated to accommodate at most one exosome and individually imaged in real time, enabling the label-free recording of digital responses in a highly multiplexed geometry. This approach results in a three orders of magnitude sensitivity improvement over previously reported real-time, multiplexed platforms. Each nanosensor is elevated atop a quartz nanopillar, minimizing unwanted nonspecific substrate binding contributions. The approach is validated with the detection of exosomes secreted by MCF7 breast adenocarcinoma cells. We demonstrate the increasingly digital and stochastic nature of the response as the number of subsampled nanosensors is reduced from four hundred to one. Public Library of Science 2018-08-24 /pmc/articles/PMC6108516/ /pubmed/30142169 http://dx.doi.org/10.1371/journal.pone.0202773 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Raghu, Deepa
Christodoulides, Joseph A.
Christophersen, Marc
Liu, Jinny L.
Anderson, George P.
Robitaille, Michael
Byers, Jeff M.
Raphael, Marc P.
Nanoplasmonic pillars engineered for single exosome detection
title Nanoplasmonic pillars engineered for single exosome detection
title_full Nanoplasmonic pillars engineered for single exosome detection
title_fullStr Nanoplasmonic pillars engineered for single exosome detection
title_full_unstemmed Nanoplasmonic pillars engineered for single exosome detection
title_short Nanoplasmonic pillars engineered for single exosome detection
title_sort nanoplasmonic pillars engineered for single exosome detection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108516/
https://www.ncbi.nlm.nih.gov/pubmed/30142169
http://dx.doi.org/10.1371/journal.pone.0202773
work_keys_str_mv AT raghudeepa nanoplasmonicpillarsengineeredforsingleexosomedetection
AT christodoulidesjosepha nanoplasmonicpillarsengineeredforsingleexosomedetection
AT christophersenmarc nanoplasmonicpillarsengineeredforsingleexosomedetection
AT liujinnyl nanoplasmonicpillarsengineeredforsingleexosomedetection
AT andersongeorgep nanoplasmonicpillarsengineeredforsingleexosomedetection
AT robitaillemichael nanoplasmonicpillarsengineeredforsingleexosomedetection
AT byersjeffm nanoplasmonicpillarsengineeredforsingleexosomedetection
AT raphaelmarcp nanoplasmonicpillarsengineeredforsingleexosomedetection