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Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber
PURPOSE: To compare the accuracy of Doppler optical coherence tomography (DOCT) and OCT angiography (OCTA) for measuring retinal blood vessel caliber at different flow rates. METHODS: A research-grade 1060-nm OCT system with 3.5-μm axial resolution in retinal tissue and 92,000 A scan/s image acquisi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108529/ https://www.ncbi.nlm.nih.gov/pubmed/30159211 http://dx.doi.org/10.1167/tvst.7.4.18 |
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author | Hosseinaee, Zohreh Tan, Bingyao Martinez, Adam Bizheva, Kostadinka K. |
author_facet | Hosseinaee, Zohreh Tan, Bingyao Martinez, Adam Bizheva, Kostadinka K. |
author_sort | Hosseinaee, Zohreh |
collection | PubMed |
description | PURPOSE: To compare the accuracy of Doppler optical coherence tomography (DOCT) and OCT angiography (OCTA) for measuring retinal blood vessel caliber at different flow rates. METHODS: A research-grade 1060-nm OCT system with 3.5-μm axial resolution in retinal tissue and 92,000 A scan/s image acquisition rate was used in this study. DOCT and OCTA measurements were acquired both from a flow phantom and in vivo from retinal blood vessels in six male Brown Norway rats. The total retinal blood flow (TRBF) was modified from baseline to 70% and 20% of baseline by reducing the ocular perfusion pressure (OPP). The retinal blood vessel caliber (RBVC) was measured from OCTA and DOCT images. The caliber measurements were conducted by two separate graders using a custom MATLAB-based image processing algorithm. RESULTS: The RBVC measured with OCTA and DOCT for normal blood flow rates were not significantly different (56.69 ± 12.17 and 57.17 ± 9.46 μm, P = 0.27, respectively). However, significant differences were detected when TRBF was reduced to 70% (55.69 ± 11.56 vs. 50.62 ± 8.85 μm, P < 0.01) and 20% (50.29 ± 9.29 vs. 44.88 ± 7.13 μm, P < 0.01) of baseline. CONCLUSIONS: Reduced TRBF resulted in inaccuracy of the RBVC measurements with DOCT in both the phantom and animal study. This result suggests that OCTA is a more accurate tool for RBVC evaluation when applied to retinal diseases associated with reduced TRBF, such as glaucoma and diabetic retinopathy. TRANSLATIONAL RELEVANCE: Results from this study are directly applicable to clinical studies of retinal blood flow measured with OCTA and DOCT. |
format | Online Article Text |
id | pubmed-6108529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-61085292018-08-29 Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber Hosseinaee, Zohreh Tan, Bingyao Martinez, Adam Bizheva, Kostadinka K. Transl Vis Sci Technol Articles PURPOSE: To compare the accuracy of Doppler optical coherence tomography (DOCT) and OCT angiography (OCTA) for measuring retinal blood vessel caliber at different flow rates. METHODS: A research-grade 1060-nm OCT system with 3.5-μm axial resolution in retinal tissue and 92,000 A scan/s image acquisition rate was used in this study. DOCT and OCTA measurements were acquired both from a flow phantom and in vivo from retinal blood vessels in six male Brown Norway rats. The total retinal blood flow (TRBF) was modified from baseline to 70% and 20% of baseline by reducing the ocular perfusion pressure (OPP). The retinal blood vessel caliber (RBVC) was measured from OCTA and DOCT images. The caliber measurements were conducted by two separate graders using a custom MATLAB-based image processing algorithm. RESULTS: The RBVC measured with OCTA and DOCT for normal blood flow rates were not significantly different (56.69 ± 12.17 and 57.17 ± 9.46 μm, P = 0.27, respectively). However, significant differences were detected when TRBF was reduced to 70% (55.69 ± 11.56 vs. 50.62 ± 8.85 μm, P < 0.01) and 20% (50.29 ± 9.29 vs. 44.88 ± 7.13 μm, P < 0.01) of baseline. CONCLUSIONS: Reduced TRBF resulted in inaccuracy of the RBVC measurements with DOCT in both the phantom and animal study. This result suggests that OCTA is a more accurate tool for RBVC evaluation when applied to retinal diseases associated with reduced TRBF, such as glaucoma and diabetic retinopathy. TRANSLATIONAL RELEVANCE: Results from this study are directly applicable to clinical studies of retinal blood flow measured with OCTA and DOCT. The Association for Research in Vision and Ophthalmology 2018-08-24 /pmc/articles/PMC6108529/ /pubmed/30159211 http://dx.doi.org/10.1167/tvst.7.4.18 Text en Copyright 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Articles Hosseinaee, Zohreh Tan, Bingyao Martinez, Adam Bizheva, Kostadinka K. Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber |
title | Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber |
title_full | Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber |
title_fullStr | Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber |
title_full_unstemmed | Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber |
title_short | Comparative Study of Optical Coherence Tomography Angiography and Phase-Resolved Doppler Optical Coherence Tomography for Measurement of Retinal Blood Vessels Caliber |
title_sort | comparative study of optical coherence tomography angiography and phase-resolved doppler optical coherence tomography for measurement of retinal blood vessels caliber |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108529/ https://www.ncbi.nlm.nih.gov/pubmed/30159211 http://dx.doi.org/10.1167/tvst.7.4.18 |
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