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Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway

Mitogen-activated protein kinase-activated protein kinase 2 (MK2) and its mediated inflammation are involved in various diseases, including spinal cord injury (SCI). Ultrashortwave (USW) radiation has previously been reported to exert a protective effect on SCI. In the present study, through a serie...

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Autores principales: Wang, Nan, Feng, Zhiping, Zhao, Wei, Zhang, Zhiqiang, Zhang, Lixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108855/
https://www.ncbi.nlm.nih.gov/pubmed/30066830
http://dx.doi.org/10.3892/ijmm.2018.3786
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author Wang, Nan
Feng, Zhiping
Zhao, Wei
Zhang, Zhiqiang
Zhang, Lixin
author_facet Wang, Nan
Feng, Zhiping
Zhao, Wei
Zhang, Zhiqiang
Zhang, Lixin
author_sort Wang, Nan
collection PubMed
description Mitogen-activated protein kinase-activated protein kinase 2 (MK2) and its mediated inflammation are involved in various diseases, including spinal cord injury (SCI). Ultrashortwave (USW) radiation has previously been reported to exert a protective effect on SCI. In the present study, through a series of reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot and immunofluorescence assay, it was found that MK2 and tumor necrosis factor (TNF)-α/interleukin (IL)-1β were elevated in patients with SCI and in H(2)O(2)-treated C8-D1A cells. Through gene level and protein level detection by using of RT-qPCR, western blot, immunofluorescence assay and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling assay, it was demonstrated that USW radiation inhibited the expression of MK2/TNF-α/IL-1β and suppressed the apoptosis of H(2)O(2)-treated C8-D1A cells. Furthermore, it was confirmed that the overexpression of MK2 reversed the protective effect of USW on C8-D1A cells, which indicated that USW achieved its function via regulation of the MK2/TNF-α/IL-1β pathway. Finally, using a constructed in vivo model and a series of RT-qPCR, western blot and IHC detection, it was confirmed that USW suppressed the expression of MK2 to promote functional recovery following SCI. The findings of the present study may provide a novel target and improve on the current understanding of how USW functions in the treatment of SCI.
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spelling pubmed-61088552018-08-27 Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway Wang, Nan Feng, Zhiping Zhao, Wei Zhang, Zhiqiang Zhang, Lixin Int J Mol Med Articles Mitogen-activated protein kinase-activated protein kinase 2 (MK2) and its mediated inflammation are involved in various diseases, including spinal cord injury (SCI). Ultrashortwave (USW) radiation has previously been reported to exert a protective effect on SCI. In the present study, through a series of reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot and immunofluorescence assay, it was found that MK2 and tumor necrosis factor (TNF)-α/interleukin (IL)-1β were elevated in patients with SCI and in H(2)O(2)-treated C8-D1A cells. Through gene level and protein level detection by using of RT-qPCR, western blot, immunofluorescence assay and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling assay, it was demonstrated that USW radiation inhibited the expression of MK2/TNF-α/IL-1β and suppressed the apoptosis of H(2)O(2)-treated C8-D1A cells. Furthermore, it was confirmed that the overexpression of MK2 reversed the protective effect of USW on C8-D1A cells, which indicated that USW achieved its function via regulation of the MK2/TNF-α/IL-1β pathway. Finally, using a constructed in vivo model and a series of RT-qPCR, western blot and IHC detection, it was confirmed that USW suppressed the expression of MK2 to promote functional recovery following SCI. The findings of the present study may provide a novel target and improve on the current understanding of how USW functions in the treatment of SCI. D.A. Spandidos 2018-10 2018-07-19 /pmc/articles/PMC6108855/ /pubmed/30066830 http://dx.doi.org/10.3892/ijmm.2018.3786 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Nan
Feng, Zhiping
Zhao, Wei
Zhang, Zhiqiang
Zhang, Lixin
Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway
title Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway
title_full Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway
title_fullStr Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway
title_full_unstemmed Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway
title_short Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF-α pathway
title_sort ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the mk2/tnf-α pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108855/
https://www.ncbi.nlm.nih.gov/pubmed/30066830
http://dx.doi.org/10.3892/ijmm.2018.3786
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