Cargando…

Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin

Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450  ppm) was isolated from the soil of pesticide con...

Descripción completa

Detalles Bibliográficos
Autores principales: Gangola, Saurabh, Sharma, Anita, Bhatt, Pankaj, Khati, Priyanka, Chaudhary, Parul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109164/
https://www.ncbi.nlm.nih.gov/pubmed/30143738
http://dx.doi.org/10.1038/s41598-018-31082-5
_version_ 1783350273423441920
author Gangola, Saurabh
Sharma, Anita
Bhatt, Pankaj
Khati, Priyanka
Chaudhary, Parul
author_facet Gangola, Saurabh
Sharma, Anita
Bhatt, Pankaj
Khati, Priyanka
Chaudhary, Parul
author_sort Gangola, Saurabh
collection PubMed
description Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450  ppm) was isolated from the soil of pesticide contaminated agriculture field and characterized by using polyphasic approach. On molecular basis bacterial isolate showed 98% homology with Bacillus subtilis strain 1D. Under optimized growth conditions, bacteria showed 95% degradation of cypermethrin after 15 days and the end products of cypermethrin biodegradation under aerobic conditions were cyclododecylamine, phenol, 3-(2,2-dichloroethenyl 2,2-dimethyl cyclopropane carboxylate,1-decanol,chloroacetic acid, acetic acid, cyclopentan palmitoleic acid, and decanoic acid. Amplification of esterase (700 bp) and laccase (1200 bp) genes was confirmed by PCR which showed a possible role of these enzymes in biodegradation of cypermethrin. In the presence of cypermethrin Km value(s) of both the enzymes was low than the control. A nobel cypermethrin degradation pathway followed by B. subtilis was proposed on the basis of characterization of biodegraded products of cypermethrin using GC-MS. Cypermethrin biodegradation ability of Bacillus subtilis strain 1D without producing any toxic end product reveals the potential of this organism in cleaning of pesticide contaminated soil and water.
format Online
Article
Text
id pubmed-6109164
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61091642018-08-31 Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin Gangola, Saurabh Sharma, Anita Bhatt, Pankaj Khati, Priyanka Chaudhary, Parul Sci Rep Article Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450  ppm) was isolated from the soil of pesticide contaminated agriculture field and characterized by using polyphasic approach. On molecular basis bacterial isolate showed 98% homology with Bacillus subtilis strain 1D. Under optimized growth conditions, bacteria showed 95% degradation of cypermethrin after 15 days and the end products of cypermethrin biodegradation under aerobic conditions were cyclododecylamine, phenol, 3-(2,2-dichloroethenyl 2,2-dimethyl cyclopropane carboxylate,1-decanol,chloroacetic acid, acetic acid, cyclopentan palmitoleic acid, and decanoic acid. Amplification of esterase (700 bp) and laccase (1200 bp) genes was confirmed by PCR which showed a possible role of these enzymes in biodegradation of cypermethrin. In the presence of cypermethrin Km value(s) of both the enzymes was low than the control. A nobel cypermethrin degradation pathway followed by B. subtilis was proposed on the basis of characterization of biodegraded products of cypermethrin using GC-MS. Cypermethrin biodegradation ability of Bacillus subtilis strain 1D without producing any toxic end product reveals the potential of this organism in cleaning of pesticide contaminated soil and water. Nature Publishing Group UK 2018-08-24 /pmc/articles/PMC6109164/ /pubmed/30143738 http://dx.doi.org/10.1038/s41598-018-31082-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gangola, Saurabh
Sharma, Anita
Bhatt, Pankaj
Khati, Priyanka
Chaudhary, Parul
Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
title Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
title_full Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
title_fullStr Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
title_full_unstemmed Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
title_short Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
title_sort presence of esterase and laccase in bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109164/
https://www.ncbi.nlm.nih.gov/pubmed/30143738
http://dx.doi.org/10.1038/s41598-018-31082-5
work_keys_str_mv AT gangolasaurabh presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin
AT sharmaanita presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin
AT bhattpankaj presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin
AT khatipriyanka presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin
AT chaudharyparul presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin