Cargando…
Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin
Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450 ppm) was isolated from the soil of pesticide con...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109164/ https://www.ncbi.nlm.nih.gov/pubmed/30143738 http://dx.doi.org/10.1038/s41598-018-31082-5 |
_version_ | 1783350273423441920 |
---|---|
author | Gangola, Saurabh Sharma, Anita Bhatt, Pankaj Khati, Priyanka Chaudhary, Parul |
author_facet | Gangola, Saurabh Sharma, Anita Bhatt, Pankaj Khati, Priyanka Chaudhary, Parul |
author_sort | Gangola, Saurabh |
collection | PubMed |
description | Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450 ppm) was isolated from the soil of pesticide contaminated agriculture field and characterized by using polyphasic approach. On molecular basis bacterial isolate showed 98% homology with Bacillus subtilis strain 1D. Under optimized growth conditions, bacteria showed 95% degradation of cypermethrin after 15 days and the end products of cypermethrin biodegradation under aerobic conditions were cyclododecylamine, phenol, 3-(2,2-dichloroethenyl 2,2-dimethyl cyclopropane carboxylate,1-decanol,chloroacetic acid, acetic acid, cyclopentan palmitoleic acid, and decanoic acid. Amplification of esterase (700 bp) and laccase (1200 bp) genes was confirmed by PCR which showed a possible role of these enzymes in biodegradation of cypermethrin. In the presence of cypermethrin Km value(s) of both the enzymes was low than the control. A nobel cypermethrin degradation pathway followed by B. subtilis was proposed on the basis of characterization of biodegraded products of cypermethrin using GC-MS. Cypermethrin biodegradation ability of Bacillus subtilis strain 1D without producing any toxic end product reveals the potential of this organism in cleaning of pesticide contaminated soil and water. |
format | Online Article Text |
id | pubmed-6109164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61091642018-08-31 Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin Gangola, Saurabh Sharma, Anita Bhatt, Pankaj Khati, Priyanka Chaudhary, Parul Sci Rep Article Ubiquitous presence of cypermethrin as a contaminant in surface stream and soil necessitates to develop potential bioremediation methods to degrade and eliminate this pollutant from the environment. A cypermethrin utilizing bacterial strain (MIC, 450 ppm) was isolated from the soil of pesticide contaminated agriculture field and characterized by using polyphasic approach. On molecular basis bacterial isolate showed 98% homology with Bacillus subtilis strain 1D. Under optimized growth conditions, bacteria showed 95% degradation of cypermethrin after 15 days and the end products of cypermethrin biodegradation under aerobic conditions were cyclododecylamine, phenol, 3-(2,2-dichloroethenyl 2,2-dimethyl cyclopropane carboxylate,1-decanol,chloroacetic acid, acetic acid, cyclopentan palmitoleic acid, and decanoic acid. Amplification of esterase (700 bp) and laccase (1200 bp) genes was confirmed by PCR which showed a possible role of these enzymes in biodegradation of cypermethrin. In the presence of cypermethrin Km value(s) of both the enzymes was low than the control. A nobel cypermethrin degradation pathway followed by B. subtilis was proposed on the basis of characterization of biodegraded products of cypermethrin using GC-MS. Cypermethrin biodegradation ability of Bacillus subtilis strain 1D without producing any toxic end product reveals the potential of this organism in cleaning of pesticide contaminated soil and water. Nature Publishing Group UK 2018-08-24 /pmc/articles/PMC6109164/ /pubmed/30143738 http://dx.doi.org/10.1038/s41598-018-31082-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gangola, Saurabh Sharma, Anita Bhatt, Pankaj Khati, Priyanka Chaudhary, Parul Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
title | Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
title_full | Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
title_fullStr | Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
title_full_unstemmed | Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
title_short | Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
title_sort | presence of esterase and laccase in bacillus subtilis facilitates biodegradation and detoxification of cypermethrin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109164/ https://www.ncbi.nlm.nih.gov/pubmed/30143738 http://dx.doi.org/10.1038/s41598-018-31082-5 |
work_keys_str_mv | AT gangolasaurabh presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin AT sharmaanita presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin AT bhattpankaj presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin AT khatipriyanka presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin AT chaudharyparul presenceofesteraseandlaccaseinbacillussubtilisfacilitatesbiodegradationanddetoxificationofcypermethrin |