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Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm
Tomato yellow leaf curl virus (TYLCV) is a virus species causing epidemics in tomato (Solanum lycopersicum) worldwide. Many efforts have been focused on identification of resistance sources by screening wild tomato species. In many cases, the accession numbers were either not provided in publication...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110163/ https://www.ncbi.nlm.nih.gov/pubmed/30177938 http://dx.doi.org/10.3389/fpls.2018.01198 |
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author | Yan, Zhe Pérez-de-Castro, Ana Díez, Maria J. Hutton, Samuel F. Visser, Richard G. F. Wolters, Anne-Marie A. Bai, Yuling Li, Junming |
author_facet | Yan, Zhe Pérez-de-Castro, Ana Díez, Maria J. Hutton, Samuel F. Visser, Richard G. F. Wolters, Anne-Marie A. Bai, Yuling Li, Junming |
author_sort | Yan, Zhe |
collection | PubMed |
description | Tomato yellow leaf curl virus (TYLCV) is a virus species causing epidemics in tomato (Solanum lycopersicum) worldwide. Many efforts have been focused on identification of resistance sources by screening wild tomato species. In many cases, the accession numbers were either not provided in publications or not provided in a consistent manner, which led to redundant screenings. In the current study, we summarized efforts on the screenings of wild tomato species for TYLCV resistance from various publications. In addition, we screened 708 accessions from 13 wild tomato species using different inoculation assays (i.e., whitefly natural infection and Agrobacterium-mediated inoculation) from which 138 accessions exhibited no tomato yellow leaf curl disease (TYLCD) symptoms. These symptomless accessions include 14 accessions from S. arcanum, 43 from S. chilense, 1 from S. chmielewskii, 28 from S. corneliomulleri, 5 from S. habrochaites, 4 from S. huaylasense, 2 from S. neorickii, 1 from S. pennellii, 39 from S. peruvianum, and 1 from S. pimpinellifolium. Most of the screened S. chilense accessions remained symptomless. Many symptomless accessions were also identified in S. arcanum, S. corneliomulleri, and S. peruvianum. A large number of S. pimpinellifolium accessions were screened. However, almost all of the tested accessions showed TYLCD symptoms. Further, we studied allelic variation of the Ty-1/Ty-3 gene in few S. chilense accessions by applying virus-induced gene silencing and allele mining, leading to identification of a number of allele-specific polymorphisms. Taken together, we present a comprehensive overview on TYLCV resistance and susceptibility in wild tomato germplasm, and demonstrate how to study allelic variants of the cloned Ty-genes in TYLCV-resistant accessions. |
format | Online Article Text |
id | pubmed-6110163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61101632018-09-03 Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm Yan, Zhe Pérez-de-Castro, Ana Díez, Maria J. Hutton, Samuel F. Visser, Richard G. F. Wolters, Anne-Marie A. Bai, Yuling Li, Junming Front Plant Sci Plant Science Tomato yellow leaf curl virus (TYLCV) is a virus species causing epidemics in tomato (Solanum lycopersicum) worldwide. Many efforts have been focused on identification of resistance sources by screening wild tomato species. In many cases, the accession numbers were either not provided in publications or not provided in a consistent manner, which led to redundant screenings. In the current study, we summarized efforts on the screenings of wild tomato species for TYLCV resistance from various publications. In addition, we screened 708 accessions from 13 wild tomato species using different inoculation assays (i.e., whitefly natural infection and Agrobacterium-mediated inoculation) from which 138 accessions exhibited no tomato yellow leaf curl disease (TYLCD) symptoms. These symptomless accessions include 14 accessions from S. arcanum, 43 from S. chilense, 1 from S. chmielewskii, 28 from S. corneliomulleri, 5 from S. habrochaites, 4 from S. huaylasense, 2 from S. neorickii, 1 from S. pennellii, 39 from S. peruvianum, and 1 from S. pimpinellifolium. Most of the screened S. chilense accessions remained symptomless. Many symptomless accessions were also identified in S. arcanum, S. corneliomulleri, and S. peruvianum. A large number of S. pimpinellifolium accessions were screened. However, almost all of the tested accessions showed TYLCD symptoms. Further, we studied allelic variation of the Ty-1/Ty-3 gene in few S. chilense accessions by applying virus-induced gene silencing and allele mining, leading to identification of a number of allele-specific polymorphisms. Taken together, we present a comprehensive overview on TYLCV resistance and susceptibility in wild tomato germplasm, and demonstrate how to study allelic variants of the cloned Ty-genes in TYLCV-resistant accessions. Frontiers Media S.A. 2018-08-20 /pmc/articles/PMC6110163/ /pubmed/30177938 http://dx.doi.org/10.3389/fpls.2018.01198 Text en Copyright © 2018 Yan, Pérez-de-Castro, Díez, Hutton, Visser, Wolters, Bai and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yan, Zhe Pérez-de-Castro, Ana Díez, Maria J. Hutton, Samuel F. Visser, Richard G. F. Wolters, Anne-Marie A. Bai, Yuling Li, Junming Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm |
title | Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm |
title_full | Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm |
title_fullStr | Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm |
title_full_unstemmed | Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm |
title_short | Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm |
title_sort | resistance to tomato yellow leaf curl virus in tomato germplasm |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110163/ https://www.ncbi.nlm.nih.gov/pubmed/30177938 http://dx.doi.org/10.3389/fpls.2018.01198 |
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