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SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells
While biophysical stimuli from polymeric matrices are known to significantly affect the fates of human mesenchymal stem cells (hMSCs), the stimulatory effects of nano-sized silicon-based matrices on hMSCs have not been thoroughly investigated. We previously demonstrated that vertically aligned, sing...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110734/ https://www.ncbi.nlm.nih.gov/pubmed/30150652 http://dx.doi.org/10.1038/s41598-018-30854-3 |
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author | Lin, Hsin-I Kuo, Shu-Wen Yen, Ta-Jen Lee, Oscar K. |
author_facet | Lin, Hsin-I Kuo, Shu-Wen Yen, Ta-Jen Lee, Oscar K. |
author_sort | Lin, Hsin-I |
collection | PubMed |
description | While biophysical stimuli from polymeric matrices are known to significantly affect the fates of human mesenchymal stem cells (hMSCs), the stimulatory effects of nano-sized silicon-based matrices on hMSCs have not been thoroughly investigated. We previously demonstrated that vertically aligned, single-crystalline silicon nanowires (SiNWs) can control the osteogenicity of hMSCs via controllable spring constants from SiNWs matrix. However, other possible differentiation fates of hMSCs on SiNWs have not been explored. We hypothesize that tunable spring constant from artificial SiNWs matrices can direct different types of hMSC differentiations. The spring constants of tunable SiNW matrices can be consistently controlled by tuning the SiNW length. The results of gene expression and cell stiffness suggest that hMSCs differentiations are sensitive to our distinguishable spring constants from the SiNWs groups, and simultaneously conduct osteogenicity and adipogenicity. These findings suggest that SiNW matrices can regulate the fates of hMSCs when the SiNW characteristics are carefully tuned. |
format | Online Article Text |
id | pubmed-6110734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61107342018-08-30 SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells Lin, Hsin-I Kuo, Shu-Wen Yen, Ta-Jen Lee, Oscar K. Sci Rep Article While biophysical stimuli from polymeric matrices are known to significantly affect the fates of human mesenchymal stem cells (hMSCs), the stimulatory effects of nano-sized silicon-based matrices on hMSCs have not been thoroughly investigated. We previously demonstrated that vertically aligned, single-crystalline silicon nanowires (SiNWs) can control the osteogenicity of hMSCs via controllable spring constants from SiNWs matrix. However, other possible differentiation fates of hMSCs on SiNWs have not been explored. We hypothesize that tunable spring constant from artificial SiNWs matrices can direct different types of hMSC differentiations. The spring constants of tunable SiNW matrices can be consistently controlled by tuning the SiNW length. The results of gene expression and cell stiffness suggest that hMSCs differentiations are sensitive to our distinguishable spring constants from the SiNWs groups, and simultaneously conduct osteogenicity and adipogenicity. These findings suggest that SiNW matrices can regulate the fates of hMSCs when the SiNW characteristics are carefully tuned. Nature Publishing Group UK 2018-08-27 /pmc/articles/PMC6110734/ /pubmed/30150652 http://dx.doi.org/10.1038/s41598-018-30854-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lin, Hsin-I Kuo, Shu-Wen Yen, Ta-Jen Lee, Oscar K. SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells |
title | SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells |
title_full | SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells |
title_fullStr | SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells |
title_full_unstemmed | SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells |
title_short | SiNWs Biophysically Regulate the Fates of Human Mesenchymal Stem Cells |
title_sort | sinws biophysically regulate the fates of human mesenchymal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110734/ https://www.ncbi.nlm.nih.gov/pubmed/30150652 http://dx.doi.org/10.1038/s41598-018-30854-3 |
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