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Postsynaptic p47phox regulates long-term depression in the hippocampus

It is well documented that reactive oxygen species (ROS) affects neurodegeneration in the brain. Several studies also implicate ROS in the regulation of synapse function and learning and memory processes, although the precise source of ROS generation within these contexts remains to be further explo...

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Autores principales: Yi, Jee Hyun, Kim, Dong Hyun, Piers, Thomas M., Kim, Seung Chan, Whitcomb, Daniel J., Regan, Philip, Cho, Kwangwook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110736/
https://www.ncbi.nlm.nih.gov/pubmed/30181899
http://dx.doi.org/10.1038/s41421-018-0046-x
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author Yi, Jee Hyun
Kim, Dong Hyun
Piers, Thomas M.
Kim, Seung Chan
Whitcomb, Daniel J.
Regan, Philip
Cho, Kwangwook
author_facet Yi, Jee Hyun
Kim, Dong Hyun
Piers, Thomas M.
Kim, Seung Chan
Whitcomb, Daniel J.
Regan, Philip
Cho, Kwangwook
author_sort Yi, Jee Hyun
collection PubMed
description It is well documented that reactive oxygen species (ROS) affects neurodegeneration in the brain. Several studies also implicate ROS in the regulation of synapse function and learning and memory processes, although the precise source of ROS generation within these contexts remains to be further explored. Here we show that postsynaptic superoxide generation through PKCζ-activated NADPH oxidase 2 (NOX2) is critical for long-term depression (LTD) of synaptic transmission in the CA1–Shaffer collateral synapse of the rat hippocampus. Specifically, PKCζ-dependent phosphorylation of p47phox at serine 316, a NOX2 regulatory subunit, is required for LTD but is not necessary for long-term potentiation (LTP). Our data suggest that postsynaptic p47phox phosphorylation at serine 316 is a key upstream determinant for LTD and synapse weakening.
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spelling pubmed-61107362018-09-04 Postsynaptic p47phox regulates long-term depression in the hippocampus Yi, Jee Hyun Kim, Dong Hyun Piers, Thomas M. Kim, Seung Chan Whitcomb, Daniel J. Regan, Philip Cho, Kwangwook Cell Discov Article It is well documented that reactive oxygen species (ROS) affects neurodegeneration in the brain. Several studies also implicate ROS in the regulation of synapse function and learning and memory processes, although the precise source of ROS generation within these contexts remains to be further explored. Here we show that postsynaptic superoxide generation through PKCζ-activated NADPH oxidase 2 (NOX2) is critical for long-term depression (LTD) of synaptic transmission in the CA1–Shaffer collateral synapse of the rat hippocampus. Specifically, PKCζ-dependent phosphorylation of p47phox at serine 316, a NOX2 regulatory subunit, is required for LTD but is not necessary for long-term potentiation (LTP). Our data suggest that postsynaptic p47phox phosphorylation at serine 316 is a key upstream determinant for LTD and synapse weakening. Nature Publishing Group UK 2018-08-28 /pmc/articles/PMC6110736/ /pubmed/30181899 http://dx.doi.org/10.1038/s41421-018-0046-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yi, Jee Hyun
Kim, Dong Hyun
Piers, Thomas M.
Kim, Seung Chan
Whitcomb, Daniel J.
Regan, Philip
Cho, Kwangwook
Postsynaptic p47phox regulates long-term depression in the hippocampus
title Postsynaptic p47phox regulates long-term depression in the hippocampus
title_full Postsynaptic p47phox regulates long-term depression in the hippocampus
title_fullStr Postsynaptic p47phox regulates long-term depression in the hippocampus
title_full_unstemmed Postsynaptic p47phox regulates long-term depression in the hippocampus
title_short Postsynaptic p47phox regulates long-term depression in the hippocampus
title_sort postsynaptic p47phox regulates long-term depression in the hippocampus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110736/
https://www.ncbi.nlm.nih.gov/pubmed/30181899
http://dx.doi.org/10.1038/s41421-018-0046-x
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