Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology

Bloodstream infections (BSIs) are often life-threatening, and rapid identification is critical. Here, we developed a TaqMan array card (TAC) assay to detect pathogens in BSI specimens. The TAC included 30 primer/probe pairs targeting 27 species and 3 controls. Reverse transcription and 0.1% blue dex...

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Autores principales: Zhang, Chao, Zheng, Xin, Zhao, Chengna, Li, Yan, Chen, Shuiping, Liu, Gang, Wang, Chengbin, Lv, Qingyu, Liu, Peng, Zheng, Yuling, Kong, Decong, Jiang, Hua, Jiang, Yongqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110752/
https://www.ncbi.nlm.nih.gov/pubmed/30150783
http://dx.doi.org/10.1038/s41598-018-31200-3
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author Zhang, Chao
Zheng, Xin
Zhao, Chengna
Li, Yan
Chen, Shuiping
Liu, Gang
Wang, Chengbin
Lv, Qingyu
Liu, Peng
Zheng, Yuling
Kong, Decong
Jiang, Hua
Jiang, Yongqiang
author_facet Zhang, Chao
Zheng, Xin
Zhao, Chengna
Li, Yan
Chen, Shuiping
Liu, Gang
Wang, Chengbin
Lv, Qingyu
Liu, Peng
Zheng, Yuling
Kong, Decong
Jiang, Hua
Jiang, Yongqiang
author_sort Zhang, Chao
collection PubMed
description Bloodstream infections (BSIs) are often life-threatening, and rapid identification is critical. Here, we developed a TaqMan array card (TAC) assay to detect pathogens in BSI specimens. The TAC included 30 primer/probe pairs targeting 27 species and 3 controls. Reverse transcription and 0.1% blue dextran 2000 increased the TAC assay efficiency. The primer/probe pairs had a limit of detection of 10(0)–10(2) CFU/mL and a specificity of 100%. For whole blood specimens, the TAC assay showed a sensitivity and specificity of 79.4% and 99.69%, respectively. For blood culture samples, the TAC assay showed a sensitivity and specificity of 100% and 99.67%, respectively. The TAC assay could be a promising method for early detection of bloodstream infection.
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spelling pubmed-61107522018-08-30 Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology Zhang, Chao Zheng, Xin Zhao, Chengna Li, Yan Chen, Shuiping Liu, Gang Wang, Chengbin Lv, Qingyu Liu, Peng Zheng, Yuling Kong, Decong Jiang, Hua Jiang, Yongqiang Sci Rep Article Bloodstream infections (BSIs) are often life-threatening, and rapid identification is critical. Here, we developed a TaqMan array card (TAC) assay to detect pathogens in BSI specimens. The TAC included 30 primer/probe pairs targeting 27 species and 3 controls. Reverse transcription and 0.1% blue dextran 2000 increased the TAC assay efficiency. The primer/probe pairs had a limit of detection of 10(0)–10(2) CFU/mL and a specificity of 100%. For whole blood specimens, the TAC assay showed a sensitivity and specificity of 79.4% and 99.69%, respectively. For blood culture samples, the TAC assay showed a sensitivity and specificity of 100% and 99.67%, respectively. The TAC assay could be a promising method for early detection of bloodstream infection. Nature Publishing Group UK 2018-08-27 /pmc/articles/PMC6110752/ /pubmed/30150783 http://dx.doi.org/10.1038/s41598-018-31200-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Chao
Zheng, Xin
Zhao, Chengna
Li, Yan
Chen, Shuiping
Liu, Gang
Wang, Chengbin
Lv, Qingyu
Liu, Peng
Zheng, Yuling
Kong, Decong
Jiang, Hua
Jiang, Yongqiang
Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
title Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
title_full Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
title_fullStr Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
title_full_unstemmed Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
title_short Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology
title_sort detection of pathogenic microorganisms from bloodstream infection specimens using taqman array card technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110752/
https://www.ncbi.nlm.nih.gov/pubmed/30150783
http://dx.doi.org/10.1038/s41598-018-31200-3
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