Cargando…

Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits

Silica columns from PCR purification and gel extraction kits are widely used in laboratories worldwide to assist in gene cloning. However, the use of these columns can generate plastic waste that has an environmental impact due to their one-off design and massive consumption. Thus, it is important t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhou, Ying, Zhang, Yang, He, Wei, Wang, Juan, Peng, Feixia, Huang, Liyun, Zhao, Shasha, Deng, Wensheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110862/ https://www.ncbi.nlm.nih.gov/pubmed/30150610 http://dx.doi.org/10.1038/s41598-018-30316-w

Ejemplares similares

Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis
por: Biswal, Jitendra K., et al.
Publicado: (2021)

Immunoadsorption Column Reuse
por: Tiwari, Vaibhav, et al.
Publicado: (2021)

Separation and Purification of Hydroxyl-α-Sanshool from Zanthoxylum armatum DC. by Silica Gel Column Chromatography
por: Cheng, Jinxi, et al.
Publicado: (2023)

Rapid Isolation of Extracellular Vesicles Using a Hydrophilic Porous Silica Gel-Based Size-Exclusion Chromatography Column
por: Yoshitake, Jun, et al.
Publicado: (2022)

Regeneration and Reuse of Immunoaffinity Column for Highly Efficient Clean-Up and Economic Detection of Ochratoxin A in Malt and Ginger
por: Liu, Xi, et al.
Publicado: (2018)

Gel permeation chromatography as a multifunctional processor for nanocrystal purification and on-column ligand exchange chemistry
por: Shen, Yi, et al.
Publicado: (2016)

Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA
por: Deleu, Jill, et al.
Publicado: (2022)

Reusing Immunoadsorption Column – Making the ABO Incompatible Renal Transplant Affordable
por: Jha, P. K., et al.
Publicado: (2017)

Removal of Methylene Blue from Aqueous Solution by Mixture of Reused Silica Gel Desiccant and Natural Sand or Eggshell Waste
por: Juzsakova, Tatjana, et al.
Publicado: (2023)

Development and Optimization of a Silica Column-Based Extraction Protocol for Ancient DNA
por: Dehasque, Marianne, et al.
Publicado: (2022)

Loading of Silver (I) Ion in L-Cysteine-Functionalized Silica Gel Material for Aquatic Purification
por: Al-Anber, Mohammed A., et al.
Publicado: (2023)

Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature
por: Abdallah, Nermeen M.A., et al.
Publicado: (2020)

Enhancing the compatibility of BioCaRGOS silica sol-gel technology with ctDNA extraction and droplet digital PCR (ddPCR) analysis
por: Potnis, Chinmay S., et al.
Publicado: (2022)

Porous Waste Glass for Lead Removal in Packed Bed Columns and Reuse in Cement Conglomerates
por: Petrella, Andrea, et al.
Publicado: (2018)

A novel method to investigate the effects of gene mutations at the cellular level using a dual expression lentiviral vector
por: Huang, Liyun, et al.
Publicado: (2019)

Comprehensive Chemical Profiling in the Ethanol Extract of Pluchea indica Aerial Parts by Liquid Chromatography/Mass Spectrometry Analysis of Its Silica Gel Column Chromatography Fractions
por: Ruan, Jingya, et al.
Publicado: (2019)

Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products
por: Ma, Fei, et al.
Publicado: (2013)

Evaluation of five column‐based isolation kits and their ability to extract miRNA from human milk
por: Ahlberg, Emelie, et al.
Publicado: (2021)

Purification of pituitary autoantigen by column liquid chromatography and chromatofocusing
por: Gut, Paweł, et al.
Publicado: (2014)

Coupling Isotachophoresis with Affinity Chromatography for Rapid and Selective Purification with High Column Utilization, Part 1: Theory
por: Shkolnikov, Viktor, et al.
Publicado: (2014)

Determination of phthalate esters in physiological saline solution by monolithic silica spin column extraction method
por: Lu, Lu, et al.
Publicado: (2011)

Coupling Isotachophoresis with Affinity Chromatography for Rapid and Selective Purification with High Column Utilization, Part 2: Experimental Study
por: Shkolnikov, Viktor, et al.
Publicado: (2014)

The effect of column purification on cDNA indirect labelling for microarrays
por: Molas, M Lia, et al.
Publicado: (2007)

A cost-effective RNA extraction technique from animal cells and tissue using silica columns
por: Escobar, Mario D., et al.
Publicado: (2017)

Insights into Coproduction of Silica Gel via Desulfurization of Steel Slag and Silica Gel Adsorption Performance
por: Ma, Zhuohui, et al.
Publicado: (2022)

Diagnostic evaluation of qRT-PCR-based kit and dPCR-based kit for COVID-19
por: Lee, Cherl-Joon, et al.
Publicado: (2021)

Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification
por: Fukumoto, Tatsuya, et al.
Publicado: (2020)

Plasmodium falciparum Gametocyte Enrichment in Peripheral Blood Samples by Magnetic Fractionation: Gametocyte Yields and Possibilities to Reuse Columns
por: Graumans, Wouter, et al.
Publicado: (2019)

Identification and Structural Analysis of Spirostanol Saponin from Yucca schidigera by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis
por: Ruan, Jingya, et al.
Publicado: (2020)

Purification of Gst Fusion Proteins, On-Column Cleavage, and Sample Clean Up
por: Sigrell, Jill A., et al.
Publicado: (2002)

Prediction of the performance of pre‐packed purification columns through machine learning
por: Jiang, Qihao, et al.
Publicado: (2022)

To reuse or not reuse? That is a rebound question!
por: Akkara, John D, et al.
Publicado: (2023)

Sour Gas Adsorption on Silica Gels
por: Jacobs, John H., et al.
Publicado: (2023)

Update of Research Progress on Small Incision Lenticule Extraction (SMILE) Lenticule Reuse
por: Zhang, Hao, et al.
Publicado: (2023)

Purification of coated vesicles by agarose gel electrophoresis
Publicado: (1981)

In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies
por: Boulet-Audet, Maxime, et al.
Publicado: (2016)

Poly(Hydroxyethyl Methacrylate) Immunoaffinity Cryogel Column for the Purification of Human Immunoglobulin M
por: Bakhshpour, Monireh, et al.
Publicado: (2020)

Continuous countercurrent chromatographic twin‐column purification of oligonucleotides: The role of the displacement effect
por: Fioretti, Ismaele, et al.
Publicado: (2022)

A High-Performance Polymer Composite Column for Coronavirus Nucleic Acid Purification
por: Zarouri, Akli, et al.
Publicado: (2023)

Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes
por: Masaki, Kanako, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ngalbd8nj6povnnti7uf95emuf