Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells

A pull-down experiment (co-immunoprecipitation) was performed on a T24 human bladder cancer cell lysate treated with the Hsp inhibitor VER155008 using an Hsp70 antibody attached to Dynabeads. Keratin 9, a cytoskeleton intermediate filament protein, was identified by LC MS/MS analysis. This novel fin...

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Detalles Bibliográficos
Autores principales: Andolino, C., Hess, C., Prince, T., Williams, H., Chernin, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111075/
https://www.ncbi.nlm.nih.gov/pubmed/29802537
http://dx.doi.org/10.1007/s12192-018-0913-2
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author Andolino, C.
Hess, C.
Prince, T.
Williams, H.
Chernin, M.
author_facet Andolino, C.
Hess, C.
Prince, T.
Williams, H.
Chernin, M.
author_sort Andolino, C.
collection PubMed
description A pull-down experiment (co-immunoprecipitation) was performed on a T24 human bladder cancer cell lysate treated with the Hsp inhibitor VER155008 using an Hsp70 antibody attached to Dynabeads. Keratin 9, a cytoskeleton intermediate filament protein, was identified by LC MS/MS analysis. This novel finding was confirmed by Western blotting, RT-PCR, and immunocytochemistry. Other members of the keratin family of proteins have been shown to be involved in cancer progression, most recently identified to be associated with cell invasion and metastasis. The specific role of keratin 9 expression in these cells is yet to be determined.
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spelling pubmed-61110752018-08-31 Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells Andolino, C. Hess, C. Prince, T. Williams, H. Chernin, M. Cell Stress Chaperones Short Communication A pull-down experiment (co-immunoprecipitation) was performed on a T24 human bladder cancer cell lysate treated with the Hsp inhibitor VER155008 using an Hsp70 antibody attached to Dynabeads. Keratin 9, a cytoskeleton intermediate filament protein, was identified by LC MS/MS analysis. This novel finding was confirmed by Western blotting, RT-PCR, and immunocytochemistry. Other members of the keratin family of proteins have been shown to be involved in cancer progression, most recently identified to be associated with cell invasion and metastasis. The specific role of keratin 9 expression in these cells is yet to be determined. Springer Netherlands 2018-05-25 2018-09 /pmc/articles/PMC6111075/ /pubmed/29802537 http://dx.doi.org/10.1007/s12192-018-0913-2 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Short Communication
Andolino, C.
Hess, C.
Prince, T.
Williams, H.
Chernin, M.
Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells
title Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells
title_full Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells
title_fullStr Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells
title_full_unstemmed Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells
title_short Drug-induced keratin 9 interaction with Hsp70 in bladder cancer cells
title_sort drug-induced keratin 9 interaction with hsp70 in bladder cancer cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111075/
https://www.ncbi.nlm.nih.gov/pubmed/29802537
http://dx.doi.org/10.1007/s12192-018-0913-2
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