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Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene

We investigated whether the presence of anthocyanins in plants could contribute to low pH stress tolerance using anthocyanin-enriched transgenic petunia lines (PM2, PM6, and PM8) expressing RsMYB1 and wild-type (WT) plants. We examined several physiological and biochemical factors and the transcript...

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Autores principales: Naing, Aung H., Lee, Deuk B., Ai, Trinh N., Lim, Ki B., Kim, Chang K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111200/
https://www.ncbi.nlm.nih.gov/pubmed/30186290
http://dx.doi.org/10.3389/fpls.2018.01124
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author Naing, Aung H.
Lee, Deuk B.
Ai, Trinh N.
Lim, Ki B.
Kim, Chang K.
author_facet Naing, Aung H.
Lee, Deuk B.
Ai, Trinh N.
Lim, Ki B.
Kim, Chang K.
author_sort Naing, Aung H.
collection PubMed
description We investigated whether the presence of anthocyanins in plants could contribute to low pH stress tolerance using anthocyanin-enriched transgenic petunia lines (PM2, PM6, and PM8) expressing RsMYB1 and wild-type (WT) plants. We examined several physiological and biochemical factors and the transcript levels of genes involved in abiotic stress tolerance. A reduction in plant growth, including plant height and fresh weight, was observed when plants (PM2, PM6, PM8, and WT) were exposed to low pH (pH 3.0) conditions compared to growth under normal (pH 5.8) conditions. A small reduction in the growth of PM6 was observed, followed by that in PM2, PM8, and WT, reflecting the anthocyanin levels in the plants (PM6 > PM2 and PM8 > WT). An analysis of physiological and biochemical factors also supports the degree of low pH tolerance in the plants (PM6 > PM2 and PM8 > WT). In addition, an enhanced expression of the genes [superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), osmotin, and vacuolar H(+)-ATPase (V-ATPase)] was observed in the transgenic lines (PM2, PM6, and PM8). The resultant of the enhanced transcript levels of the genes could promote antioxidant activities, proline content, and pH homeostasis involved in the mechanisms underlying abiotic stress tolerance in plants. These results suggest that anthocyanin-enriched plants overexpressing RsMYB1 enhances low pH stress tolerance by elevating the transcript levels of the relevant genes.
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spelling pubmed-61112002018-09-05 Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene Naing, Aung H. Lee, Deuk B. Ai, Trinh N. Lim, Ki B. Kim, Chang K. Front Plant Sci Plant Science We investigated whether the presence of anthocyanins in plants could contribute to low pH stress tolerance using anthocyanin-enriched transgenic petunia lines (PM2, PM6, and PM8) expressing RsMYB1 and wild-type (WT) plants. We examined several physiological and biochemical factors and the transcript levels of genes involved in abiotic stress tolerance. A reduction in plant growth, including plant height and fresh weight, was observed when plants (PM2, PM6, PM8, and WT) were exposed to low pH (pH 3.0) conditions compared to growth under normal (pH 5.8) conditions. A small reduction in the growth of PM6 was observed, followed by that in PM2, PM8, and WT, reflecting the anthocyanin levels in the plants (PM6 > PM2 and PM8 > WT). An analysis of physiological and biochemical factors also supports the degree of low pH tolerance in the plants (PM6 > PM2 and PM8 > WT). In addition, an enhanced expression of the genes [superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), osmotin, and vacuolar H(+)-ATPase (V-ATPase)] was observed in the transgenic lines (PM2, PM6, and PM8). The resultant of the enhanced transcript levels of the genes could promote antioxidant activities, proline content, and pH homeostasis involved in the mechanisms underlying abiotic stress tolerance in plants. These results suggest that anthocyanin-enriched plants overexpressing RsMYB1 enhances low pH stress tolerance by elevating the transcript levels of the relevant genes. Frontiers Media S.A. 2018-08-21 /pmc/articles/PMC6111200/ /pubmed/30186290 http://dx.doi.org/10.3389/fpls.2018.01124 Text en Copyright © 2018 Naing, Lee, Ai, Lim and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Naing, Aung H.
Lee, Deuk B.
Ai, Trinh N.
Lim, Ki B.
Kim, Chang K.
Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene
title Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene
title_full Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene
title_fullStr Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene
title_full_unstemmed Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene
title_short Enhancement of Low pH Stress Tolerance in Anthocyanin-Enriched Transgenic Petunia Overexpressing RsMYB1 Gene
title_sort enhancement of low ph stress tolerance in anthocyanin-enriched transgenic petunia overexpressing rsmyb1 gene
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111200/
https://www.ncbi.nlm.nih.gov/pubmed/30186290
http://dx.doi.org/10.3389/fpls.2018.01124
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