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Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles

A new colorimetric detection of methylmercury (CH(3)Hg(+)) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (H(T7)) recognizing CH(3)Hg(+), which was used to capture...

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Autores principales: Xie, Zheng-Jun, Bao, Xian-Yu, Peng, Chi-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111283/
https://www.ncbi.nlm.nih.gov/pubmed/30111699
http://dx.doi.org/10.3390/s18082679
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author Xie, Zheng-Jun
Bao, Xian-Yu
Peng, Chi-Fang
author_facet Xie, Zheng-Jun
Bao, Xian-Yu
Peng, Chi-Fang
author_sort Xie, Zheng-Jun
collection PubMed
description A new colorimetric detection of methylmercury (CH(3)Hg(+)) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (H(T7)) recognizing CH(3)Hg(+), which was used to capture and separate CH(3)Hg(+) by centrifugation. It was found that the CH(3)Hg(+) reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H(2)O(2) was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH(3)Hg(+) with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH(3)Hg(+) detection in environmental samples.
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spelling pubmed-61112832018-08-30 Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles Xie, Zheng-Jun Bao, Xian-Yu Peng, Chi-Fang Sensors (Basel) Article A new colorimetric detection of methylmercury (CH(3)Hg(+)) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (H(T7)) recognizing CH(3)Hg(+), which was used to capture and separate CH(3)Hg(+) by centrifugation. It was found that the CH(3)Hg(+) reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H(2)O(2) was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH(3)Hg(+) with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH(3)Hg(+) detection in environmental samples. MDPI 2018-08-15 /pmc/articles/PMC6111283/ /pubmed/30111699 http://dx.doi.org/10.3390/s18082679 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Zheng-Jun
Bao, Xian-Yu
Peng, Chi-Fang
Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles
title Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles
title_full Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles
title_fullStr Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles
title_full_unstemmed Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles
title_short Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles
title_sort highly sensitive and selective colorimetric detection of methylmercury based on dna functionalized gold nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111283/
https://www.ncbi.nlm.nih.gov/pubmed/30111699
http://dx.doi.org/10.3390/s18082679
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