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miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression

The microRNAs (miRNAs/miRs) involved in the carcinogenesis and progression of malignant melanoma (MM) remain unclear. In the present study, miR-590-5p was identified to be upregulated in MM cells compared with human melanocytes using a reverse transcription-quantitative polymerase chain reaction to...

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Autores principales: Mou, Kuanhou, Ding, Meiling, Han, Dan, Zhou, Yan, Mu, Xin, Liu, Wenli, Wang, Lijuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111548/
https://www.ncbi.nlm.nih.gov/pubmed/30106445
http://dx.doi.org/10.3892/or.2018.6633
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author Mou, Kuanhou
Ding, Meiling
Han, Dan
Zhou, Yan
Mu, Xin
Liu, Wenli
Wang, Lijuan
author_facet Mou, Kuanhou
Ding, Meiling
Han, Dan
Zhou, Yan
Mu, Xin
Liu, Wenli
Wang, Lijuan
author_sort Mou, Kuanhou
collection PubMed
description The microRNAs (miRNAs/miRs) involved in the carcinogenesis and progression of malignant melanoma (MM) remain unclear. In the present study, miR-590-5p was identified to be upregulated in MM cells compared with human melanocytes using a reverse transcription-quantitative polymerase chain reaction to screen established oncogenic and tumor suppressor miRNAs. miR-590-5p was demonstrated to inhibit the cell proliferation and tumor growth of MM cells in vitro and in vivo by performing Cell Counting Kit-8 and tumour xenograft assays, respectively. In addition, flowcytometry assays indicated that miR-590-5p induced cell apoptosis and cell cycle arrest at the G1 stage in MM cells. Finally, luciferase assays and western blot analysis results confirmed that the transcriptional regulator Yes-associated protein 1 (YAP1) is upregulated and inversely associated with miR-590-5p expression in MM cells, and is the direct target and functional mediator of miR-590-5p in MM. Altogether these results reveal the functional and mechanistic link between miR-590-5p and YAP1 in the progression of MM. Therefore, miR-590-5p is a potential therapeutic target in MM.
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spelling pubmed-61115482018-08-30 miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression Mou, Kuanhou Ding, Meiling Han, Dan Zhou, Yan Mu, Xin Liu, Wenli Wang, Lijuan Oncol Rep Articles The microRNAs (miRNAs/miRs) involved in the carcinogenesis and progression of malignant melanoma (MM) remain unclear. In the present study, miR-590-5p was identified to be upregulated in MM cells compared with human melanocytes using a reverse transcription-quantitative polymerase chain reaction to screen established oncogenic and tumor suppressor miRNAs. miR-590-5p was demonstrated to inhibit the cell proliferation and tumor growth of MM cells in vitro and in vivo by performing Cell Counting Kit-8 and tumour xenograft assays, respectively. In addition, flowcytometry assays indicated that miR-590-5p induced cell apoptosis and cell cycle arrest at the G1 stage in MM cells. Finally, luciferase assays and western blot analysis results confirmed that the transcriptional regulator Yes-associated protein 1 (YAP1) is upregulated and inversely associated with miR-590-5p expression in MM cells, and is the direct target and functional mediator of miR-590-5p in MM. Altogether these results reveal the functional and mechanistic link between miR-590-5p and YAP1 in the progression of MM. Therefore, miR-590-5p is a potential therapeutic target in MM. D.A. Spandidos 2018-10 2018-08-07 /pmc/articles/PMC6111548/ /pubmed/30106445 http://dx.doi.org/10.3892/or.2018.6633 Text en Copyright: © Mou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Mou, Kuanhou
Ding, Meiling
Han, Dan
Zhou, Yan
Mu, Xin
Liu, Wenli
Wang, Lijuan
miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression
title miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression
title_full miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression
title_fullStr miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression
title_full_unstemmed miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression
title_short miR-590-5p inhibits tumor growth in malignant melanoma by suppressing YAP1 expression
title_sort mir-590-5p inhibits tumor growth in malignant melanoma by suppressing yap1 expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111548/
https://www.ncbi.nlm.nih.gov/pubmed/30106445
http://dx.doi.org/10.3892/or.2018.6633
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