Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans

The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characteri...

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Detalles Bibliográficos
Autores principales: Su, Han, Li, Shaopei, Terebiznik, Mauricio, Guyard, Cyril, Kerman, Kagan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111780/
https://www.ncbi.nlm.nih.gov/pubmed/30110899
http://dx.doi.org/10.3390/s18082668
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author Su, Han
Li, Shaopei
Terebiznik, Mauricio
Guyard, Cyril
Kerman, Kagan
author_facet Su, Han
Li, Shaopei
Terebiznik, Mauricio
Guyard, Cyril
Kerman, Kagan
author_sort Su, Han
collection PubMed
description The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characterize these interactions between glycosaminoglycans (GAGs) and the adhesin Lcl protein. Fucoidan displayed a high affinity (K(D) 18 nM) for Lcl protein. Chondroitin sulfate A and dermatan sulfate differ in the position of a carboxyl group replacing D-glucuronate with D-iduronate. Our results indicated that the presence of D-iduronate in dermatan sulfate strongly hindered its interaction with Lcl. These biophysical studies provided valuable information in our understanding of adhesin-ligand interactions related to Legionella pneumophila infections.
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spelling pubmed-61117802018-08-30 Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans Su, Han Li, Shaopei Terebiznik, Mauricio Guyard, Cyril Kerman, Kagan Sensors (Basel) Article The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characterize these interactions between glycosaminoglycans (GAGs) and the adhesin Lcl protein. Fucoidan displayed a high affinity (K(D) 18 nM) for Lcl protein. Chondroitin sulfate A and dermatan sulfate differ in the position of a carboxyl group replacing D-glucuronate with D-iduronate. Our results indicated that the presence of D-iduronate in dermatan sulfate strongly hindered its interaction with Lcl. These biophysical studies provided valuable information in our understanding of adhesin-ligand interactions related to Legionella pneumophila infections. MDPI 2018-08-14 /pmc/articles/PMC6111780/ /pubmed/30110899 http://dx.doi.org/10.3390/s18082668 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Su, Han
Li, Shaopei
Terebiznik, Mauricio
Guyard, Cyril
Kerman, Kagan
Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
title Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
title_full Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
title_fullStr Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
title_full_unstemmed Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
title_short Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
title_sort biosensors for the detection of interaction between legionella pneumophila collagen-like protein and glycosaminoglycans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111780/
https://www.ncbi.nlm.nih.gov/pubmed/30110899
http://dx.doi.org/10.3390/s18082668
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