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Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer
Pressure ulcer formation depends on various factors among which repetitive ischaemia/reperfusion(I/R) injury plays a vital role. Molecular hydrogen (H(2)) was reported to have protective effects on I/R injuries of various internal organs. In this study, we investigated the effects of H(2) inhalation...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111801/ https://www.ncbi.nlm.nih.gov/pubmed/29921037 http://dx.doi.org/10.1111/jcmm.13704 |
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author | Fang, Wei Wang, Guizhen Tang, Luyan Su, Huilin Chen, Huyan Liao, Wanqing Xu, Jinhua |
author_facet | Fang, Wei Wang, Guizhen Tang, Luyan Su, Huilin Chen, Huyan Liao, Wanqing Xu, Jinhua |
author_sort | Fang, Wei |
collection | PubMed |
description | Pressure ulcer formation depends on various factors among which repetitive ischaemia/reperfusion(I/R) injury plays a vital role. Molecular hydrogen (H(2)) was reported to have protective effects on I/R injuries of various internal organs. In this study, we investigated the effects of H(2) inhalation on pressure ulcer and the underlying mechanisms. H(2) inhalation significantly reduced wound area, 8‐oxo‐dG level (oxidative DNA damage) and cell apoptosis rates in skin lesions. H(2) remarkably decreased ROS accumulation and enhanced antioxidant enzymes activities by up‐regulating expression of Nrf2 and its downstream components in wound tissue and/or H(2)O(2)‐treated endothelia. Meanwhile, H(2) inhibited the overexpression of MCP‐1, E‐selectin, P‐selectin and ICAM‐1 in oxidant‐induced endothelia and reduced inflammatory cells infiltration and proinflammatory cytokines (TNF‐α, IL‐1, IL‐6 and IL‐8) production in the wound. Furthermore, H(2) promoted the expression of pro‐healing factors (IL‐22, TGF‐β, VEGF and IGF1) and inhibited the production of MMP9 in wound tissue in parallel with acceleration of cutaneous collagen synthesis. Taken together, these data indicated that H(2) inhalation suppressed the formation of pressure ulcer in a mouse model. Molecular hydrogen has potentials as a novel and alternative therapy for severe pressure ulcer. The therapeutic effects of molecular hydrogen might be related to its antioxidant, anti‐inflammatory, pro‐healing actions. |
format | Online Article Text |
id | pubmed-6111801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61118012018-09-01 Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer Fang, Wei Wang, Guizhen Tang, Luyan Su, Huilin Chen, Huyan Liao, Wanqing Xu, Jinhua J Cell Mol Med Original Articles Pressure ulcer formation depends on various factors among which repetitive ischaemia/reperfusion(I/R) injury plays a vital role. Molecular hydrogen (H(2)) was reported to have protective effects on I/R injuries of various internal organs. In this study, we investigated the effects of H(2) inhalation on pressure ulcer and the underlying mechanisms. H(2) inhalation significantly reduced wound area, 8‐oxo‐dG level (oxidative DNA damage) and cell apoptosis rates in skin lesions. H(2) remarkably decreased ROS accumulation and enhanced antioxidant enzymes activities by up‐regulating expression of Nrf2 and its downstream components in wound tissue and/or H(2)O(2)‐treated endothelia. Meanwhile, H(2) inhibited the overexpression of MCP‐1, E‐selectin, P‐selectin and ICAM‐1 in oxidant‐induced endothelia and reduced inflammatory cells infiltration and proinflammatory cytokines (TNF‐α, IL‐1, IL‐6 and IL‐8) production in the wound. Furthermore, H(2) promoted the expression of pro‐healing factors (IL‐22, TGF‐β, VEGF and IGF1) and inhibited the production of MMP9 in wound tissue in parallel with acceleration of cutaneous collagen synthesis. Taken together, these data indicated that H(2) inhalation suppressed the formation of pressure ulcer in a mouse model. Molecular hydrogen has potentials as a novel and alternative therapy for severe pressure ulcer. The therapeutic effects of molecular hydrogen might be related to its antioxidant, anti‐inflammatory, pro‐healing actions. John Wiley and Sons Inc. 2018-06-19 2018-09 /pmc/articles/PMC6111801/ /pubmed/29921037 http://dx.doi.org/10.1111/jcmm.13704 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Fang, Wei Wang, Guizhen Tang, Luyan Su, Huilin Chen, Huyan Liao, Wanqing Xu, Jinhua Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
title | Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
title_full | Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
title_fullStr | Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
title_full_unstemmed | Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
title_short | Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
title_sort | hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111801/ https://www.ncbi.nlm.nih.gov/pubmed/29921037 http://dx.doi.org/10.1111/jcmm.13704 |
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