CRISPR/Cas9‐mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long‐term stability

CHO cells are the preferred host for the production of complex pharmaceutical proteins in the biopharmaceutical industry, and genome engineering of CHO cells would benefit product yield and stability. Here, we demonstrated the efficacy of a Dnmt3a‐deficient CHO cell line created by CRISPR/Cas9 genom...

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Detalles Bibliográficos
Autores principales: Jia, Yan‐Long, Guo, Xiao, Lu, Jiang‐Tao, Wang, Xiao‐Yin, Qiu, Le‐Le, Wang, Tian‐Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111867/
https://www.ncbi.nlm.nih.gov/pubmed/29851281
http://dx.doi.org/10.1111/jcmm.13687

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111867/
https://www.ncbi.nlm.nih.gov/pubmed/29851281
http://dx.doi.org/10.1111/jcmm.13687

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