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CRISPR/Cas9‐mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long‐term stability
CHO cells are the preferred host for the production of complex pharmaceutical proteins in the biopharmaceutical industry, and genome engineering of CHO cells would benefit product yield and stability. Here, we demonstrated the efficacy of a Dnmt3a‐deficient CHO cell line created by CRISPR/Cas9 genom...
Autores principales: | Jia, Yan‐Long, Guo, Xiao, Lu, Jiang‐Tao, Wang, Xiao‐Yin, Qiu, Le‐Le, Wang, Tian‐Yun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111867/ https://www.ncbi.nlm.nih.gov/pubmed/29851281 http://dx.doi.org/10.1111/jcmm.13687 |
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