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Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli

Pathogenic Escherichia coli (E. coli) widely exist in Nature and have always been a serious threat to the human health. Conventional colony forming units counting-based methods are quite time consuming and not fit for rapid detection for E. coli. Therefore, novel strategies for improving detection e...

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Detalles Bibliográficos
Autores principales: Zhao, Yu-Wen, Wang, Hai-Xia, Jia, Guang-Cheng, Li, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111995/
https://www.ncbi.nlm.nih.gov/pubmed/30071682
http://dx.doi.org/10.3390/s18082518
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author Zhao, Yu-Wen
Wang, Hai-Xia
Jia, Guang-Cheng
Li, Zheng
author_facet Zhao, Yu-Wen
Wang, Hai-Xia
Jia, Guang-Cheng
Li, Zheng
author_sort Zhao, Yu-Wen
collection PubMed
description Pathogenic Escherichia coli (E. coli) widely exist in Nature and have always been a serious threat to the human health. Conventional colony forming units counting-based methods are quite time consuming and not fit for rapid detection for E. coli. Therefore, novel strategies for improving detection efficiency and sensitivity are in great demand. Aptamers have been widely used in various sensors due to their extremely high affinity and specificity. Successful applications of aptamers have been found in the rapid detection of pathogenic E. coli. Herein, we present the latest advances in screening of aptamers for E. coli, and review the preparation and application of aptamer-based biosensors in rapid detection of E. coli. Furthermore, the problems and new trends in these aptamer-based biosensors for rapid detection of pathogenic microorganism are also discussed.
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spelling pubmed-61119952018-08-30 Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli Zhao, Yu-Wen Wang, Hai-Xia Jia, Guang-Cheng Li, Zheng Sensors (Basel) Review Pathogenic Escherichia coli (E. coli) widely exist in Nature and have always been a serious threat to the human health. Conventional colony forming units counting-based methods are quite time consuming and not fit for rapid detection for E. coli. Therefore, novel strategies for improving detection efficiency and sensitivity are in great demand. Aptamers have been widely used in various sensors due to their extremely high affinity and specificity. Successful applications of aptamers have been found in the rapid detection of pathogenic E. coli. Herein, we present the latest advances in screening of aptamers for E. coli, and review the preparation and application of aptamer-based biosensors in rapid detection of E. coli. Furthermore, the problems and new trends in these aptamer-based biosensors for rapid detection of pathogenic microorganism are also discussed. MDPI 2018-08-01 /pmc/articles/PMC6111995/ /pubmed/30071682 http://dx.doi.org/10.3390/s18082518 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zhao, Yu-Wen
Wang, Hai-Xia
Jia, Guang-Cheng
Li, Zheng
Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli
title Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli
title_full Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli
title_fullStr Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli
title_full_unstemmed Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli
title_short Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli
title_sort application of aptamer-based biosensor for rapid detection of pathogenic escherichia coli
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111995/
https://www.ncbi.nlm.nih.gov/pubmed/30071682
http://dx.doi.org/10.3390/s18082518
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