Cargando…
Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis
Rheumatoid arthritis (RA) is a disease of chronic systemic inflammation (SI). In the present study, we used four datasets to explore whether methylation-derived neutrophil-to-lymphocyte ratio (mdNLR) might be a marker of SI in new onset, untreated, and treated prevalent RA cases and/or a marker of t...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112073/ https://www.ncbi.nlm.nih.gov/pubmed/30186880 http://dx.doi.org/10.1155/2018/2624981 |
_version_ | 1783350784189005824 |
---|---|
author | Ambatipudi, Srikant Sharp, Gemma C. Clarke, Sarah L. N. Plant, Darren Tobias, Jonathan H. Evans, David M. Barton, Anne Relton, Caroline L. |
author_facet | Ambatipudi, Srikant Sharp, Gemma C. Clarke, Sarah L. N. Plant, Darren Tobias, Jonathan H. Evans, David M. Barton, Anne Relton, Caroline L. |
author_sort | Ambatipudi, Srikant |
collection | PubMed |
description | Rheumatoid arthritis (RA) is a disease of chronic systemic inflammation (SI). In the present study, we used four datasets to explore whether methylation-derived neutrophil-to-lymphocyte ratio (mdNLR) might be a marker of SI in new onset, untreated, and treated prevalent RA cases and/or a marker of treatment response to the tumour necrosis factor inhibitor (TNFi) etanercept. mdNLR was associated with increased odds of being a new onset RA case (OR = 2.32, 95% CI = 1.95–2.80, P < 2 × 10(−16)) and performed better in distinguishing new onset RA cases from controls compared to covariates: age, gender, and smoking status. In untreated preclinical RA cases and controls, mdNLR at baseline was associated with diagnosis of RA in later life after adjusting for batch (OR = 4.30, 95% CI = 1.52–21.71, P = 0.029) although no association was observed before batch correction. When prevalent RA cases were treated, there was no association with mdNLR in samples before and after batch correction (OR = 0.34, 95% CI = 0.05–1.82, P = 0.23), and mdNLR was not associated with treatment response to etanercept (OR = 1.10, 95% CI = 0.75–1.68, P = 0.64). Our results indicate that SI measured by DNA methylation data is indicative of the recent onset of RA. Although preclinical RA was associated with mdNLR, there was no difference in the mean mdNLR between preclinical RA cases and controls. mdNLR was not associated with RA case status if treatment for RA has commenced, and it is not associated with treatment response. In the future, mdNLR estimates may be used as a valuable research tool to reliably estimate SI in the absence of freshly collected blood samples. |
format | Online Article Text |
id | pubmed-6112073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-61120732018-09-05 Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis Ambatipudi, Srikant Sharp, Gemma C. Clarke, Sarah L. N. Plant, Darren Tobias, Jonathan H. Evans, David M. Barton, Anne Relton, Caroline L. J Immunol Res Research Article Rheumatoid arthritis (RA) is a disease of chronic systemic inflammation (SI). In the present study, we used four datasets to explore whether methylation-derived neutrophil-to-lymphocyte ratio (mdNLR) might be a marker of SI in new onset, untreated, and treated prevalent RA cases and/or a marker of treatment response to the tumour necrosis factor inhibitor (TNFi) etanercept. mdNLR was associated with increased odds of being a new onset RA case (OR = 2.32, 95% CI = 1.95–2.80, P < 2 × 10(−16)) and performed better in distinguishing new onset RA cases from controls compared to covariates: age, gender, and smoking status. In untreated preclinical RA cases and controls, mdNLR at baseline was associated with diagnosis of RA in later life after adjusting for batch (OR = 4.30, 95% CI = 1.52–21.71, P = 0.029) although no association was observed before batch correction. When prevalent RA cases were treated, there was no association with mdNLR in samples before and after batch correction (OR = 0.34, 95% CI = 0.05–1.82, P = 0.23), and mdNLR was not associated with treatment response to etanercept (OR = 1.10, 95% CI = 0.75–1.68, P = 0.64). Our results indicate that SI measured by DNA methylation data is indicative of the recent onset of RA. Although preclinical RA was associated with mdNLR, there was no difference in the mean mdNLR between preclinical RA cases and controls. mdNLR was not associated with RA case status if treatment for RA has commenced, and it is not associated with treatment response. In the future, mdNLR estimates may be used as a valuable research tool to reliably estimate SI in the absence of freshly collected blood samples. Hindawi 2018-08-14 /pmc/articles/PMC6112073/ /pubmed/30186880 http://dx.doi.org/10.1155/2018/2624981 Text en Copyright © 2018 Srikant Ambatipudi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ambatipudi, Srikant Sharp, Gemma C. Clarke, Sarah L. N. Plant, Darren Tobias, Jonathan H. Evans, David M. Barton, Anne Relton, Caroline L. Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis |
title | Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis |
title_full | Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis |
title_fullStr | Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis |
title_full_unstemmed | Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis |
title_short | Assessing the Role of DNA Methylation-Derived Neutrophil-to-Lymphocyte Ratio in Rheumatoid Arthritis |
title_sort | assessing the role of dna methylation-derived neutrophil-to-lymphocyte ratio in rheumatoid arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112073/ https://www.ncbi.nlm.nih.gov/pubmed/30186880 http://dx.doi.org/10.1155/2018/2624981 |
work_keys_str_mv | AT ambatipudisrikant assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT sharpgemmac assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT clarkesarahln assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT plantdarren assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT tobiasjonathanh assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT evansdavidm assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT bartonanne assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis AT reltoncarolinel assessingtheroleofdnamethylationderivedneutrophiltolymphocyteratioinrheumatoidarthritis |