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Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family

In higher plants, sugars (mainly sucrose) are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and development. Sucrose transporters, or sucrose carriers (SUCs), play an important role in the long-distance...

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Autores principales: Liu, Yuanyuan, Yin, Xin, Yang, Ya, Wang, Chuntao, Yang, Yongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112262/
https://www.ncbi.nlm.nih.gov/pubmed/30159501
http://dx.doi.org/10.1016/j.pld.2017.05.006
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author Liu, Yuanyuan
Yin, Xin
Yang, Ya
Wang, Chuntao
Yang, Yongping
author_facet Liu, Yuanyuan
Yin, Xin
Yang, Ya
Wang, Chuntao
Yang, Yongping
author_sort Liu, Yuanyuan
collection PubMed
description In higher plants, sugars (mainly sucrose) are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and development. Sucrose transporters, or sucrose carriers (SUCs), play an important role in the long-distance transportation of sucrose from source organs to sink organs, thereby affecting crop yield and quality. The identification, characterization, and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants. However, no relevant study has been reported on sucrose transporter genes in Brassica rapa var. rapa, a cruciferous root crop used mainly as vegetables and fodder. We identified and cloned 12 sucrose transporter genes from turnips, named BrrSUC1.1 to BrrSUC6.2 according to the SUC gene sequences of B. rapa pekinensis. We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified. Real-time quantitative polymerase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip. These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.
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spelling pubmed-61122622018-08-29 Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family Liu, Yuanyuan Yin, Xin Yang, Ya Wang, Chuntao Yang, Yongping Plant Divers Article In higher plants, sugars (mainly sucrose) are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and development. Sucrose transporters, or sucrose carriers (SUCs), play an important role in the long-distance transportation of sucrose from source organs to sink organs, thereby affecting crop yield and quality. The identification, characterization, and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants. However, no relevant study has been reported on sucrose transporter genes in Brassica rapa var. rapa, a cruciferous root crop used mainly as vegetables and fodder. We identified and cloned 12 sucrose transporter genes from turnips, named BrrSUC1.1 to BrrSUC6.2 according to the SUC gene sequences of B. rapa pekinensis. We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified. Real-time quantitative polymerase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip. These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips. KeAi Publishing 2017-05-20 /pmc/articles/PMC6112262/ /pubmed/30159501 http://dx.doi.org/10.1016/j.pld.2017.05.006 Text en © 2017 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Liu, Yuanyuan
Yin, Xin
Yang, Ya
Wang, Chuntao
Yang, Yongping
Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family
title Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family
title_full Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family
title_fullStr Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family
title_full_unstemmed Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family
title_short Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family
title_sort molecular cloning and expression analysis of turnip (brassica rapa var. rapa) sucrose transporter gene family
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112262/
https://www.ncbi.nlm.nih.gov/pubmed/30159501
http://dx.doi.org/10.1016/j.pld.2017.05.006
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