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Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro

The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ul...

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Autores principales: Angelieri, Fernanda, da Silva, Yuri Slusarenko, Ribeiro, Daniel Araki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112347/
https://www.ncbi.nlm.nih.gov/pubmed/30166869
http://dx.doi.org/10.1016/j.sdentj.2017.10.001
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author Angelieri, Fernanda
da Silva, Yuri Slusarenko
Ribeiro, Daniel Araki
author_facet Angelieri, Fernanda
da Silva, Yuri Slusarenko
Ribeiro, Daniel Araki
author_sort Angelieri, Fernanda
collection PubMed
description The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ultra Band Lok®) were tested in murine fibroblasts in vitro. For this purpose, eluates from each cement were prepared according manufactures instructions at 0, 2, 4, 8, 18, 32 and 64 days of immersion in artificial saliva at 37 °C. All orthodontic glass ionomer cements failed to induce cytotoxicity to murine fibroblasts for all periods evaluated in this study. However, Vidrion C® was able to induce genotoxicity after 64 days of exposure to eluates. Meron® also demonstrated genotoxicity as depicted by increasing DNA damage on 2nd day. Multicure® demonstrated genotoxicity on 32nd day and Ultra band Lok on 18th, 32nd days of exposure. Taken together, our results demonstrated that orthodontic cements derived from resin-modified glass ionomer composite (Multicure®) and compomer (Ultra Band Lok®) cause genetic damage in mammalian cells in vitro.
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spelling pubmed-61123472018-08-30 Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro Angelieri, Fernanda da Silva, Yuri Slusarenko Ribeiro, Daniel Araki Saudi Dent J Original Article The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ultra Band Lok®) were tested in murine fibroblasts in vitro. For this purpose, eluates from each cement were prepared according manufactures instructions at 0, 2, 4, 8, 18, 32 and 64 days of immersion in artificial saliva at 37 °C. All orthodontic glass ionomer cements failed to induce cytotoxicity to murine fibroblasts for all periods evaluated in this study. However, Vidrion C® was able to induce genotoxicity after 64 days of exposure to eluates. Meron® also demonstrated genotoxicity as depicted by increasing DNA damage on 2nd day. Multicure® demonstrated genotoxicity on 32nd day and Ultra band Lok on 18th, 32nd days of exposure. Taken together, our results demonstrated that orthodontic cements derived from resin-modified glass ionomer composite (Multicure®) and compomer (Ultra Band Lok®) cause genetic damage in mammalian cells in vitro. Elsevier 2018-01 2017-10-24 /pmc/articles/PMC6112347/ /pubmed/30166869 http://dx.doi.org/10.1016/j.sdentj.2017.10.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Angelieri, Fernanda
da Silva, Yuri Slusarenko
Ribeiro, Daniel Araki
Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
title Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
title_full Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
title_fullStr Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
title_full_unstemmed Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
title_short Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
title_sort genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112347/
https://www.ncbi.nlm.nih.gov/pubmed/30166869
http://dx.doi.org/10.1016/j.sdentj.2017.10.001
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