Cargando…

Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina

The mammalian retina harbors over 100 different cell types. To understand how retinal circuits work, it is essential to systematically access each type. A widely used approach for achieving targeted transgene expression exploits promoter-driven Cre lines. However, Cre expression in a given transgeni...

Descripción completa

Detalles Bibliográficos
Autores principales: Jo, Andrew, Xu, Jian, Deniz, Sercan, Cherian, Suraj, DeVries, Steven H., Zhu, Yongling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113359/
https://www.ncbi.nlm.nih.gov/pubmed/30186122
http://dx.doi.org/10.3389/fncir.2018.00066
_version_ 1783351000798593024
author Jo, Andrew
Xu, Jian
Deniz, Sercan
Cherian, Suraj
DeVries, Steven H.
Zhu, Yongling
author_facet Jo, Andrew
Xu, Jian
Deniz, Sercan
Cherian, Suraj
DeVries, Steven H.
Zhu, Yongling
author_sort Jo, Andrew
collection PubMed
description The mammalian retina harbors over 100 different cell types. To understand how retinal circuits work, it is essential to systematically access each type. A widely used approach for achieving targeted transgene expression exploits promoter-driven Cre lines. However, Cre expression in a given transgenic line in the retina and elsewhere in the brain is rarely confined to a single cell type, contributing ambiguity to the interpretation of results from broadly applied manipulations. To obtain unambiguous information about retinal processing, it is desirable to have strategies for further restricting transgene expression to a few or even to a single cell type. We employed an intersectional strategy based on a Cre/Flp double recombinase system to target amacrine and ganglion cell types in the inner retina. We analyzed expression patterns in seven Flp drivers and then created combinational mouse lines by selective cross breeding with Cre drivers. Breeding with Flp drivers can routinely remove labeling from more than 90% of the cells in Cre drivers, leading to only a handful cell types, typically 2–3, remaining in the intersection. Cre/Flp combinatorial mouse lines enabled us to identify and anatomically characterize retinal cell types with greater ease and demonstrated the feasibility of intersectional strategies in retinal research. In addition to the retina, we examined Flp expression in the lateral geniculate nucleus and superior colliculus. Our results establish a foundation for future application of intersectional strategies in the retina and retino-recipient regions.
format Online
Article
Text
id pubmed-6113359
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-61133592018-09-05 Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina Jo, Andrew Xu, Jian Deniz, Sercan Cherian, Suraj DeVries, Steven H. Zhu, Yongling Front Neural Circuits Neuroscience The mammalian retina harbors over 100 different cell types. To understand how retinal circuits work, it is essential to systematically access each type. A widely used approach for achieving targeted transgene expression exploits promoter-driven Cre lines. However, Cre expression in a given transgenic line in the retina and elsewhere in the brain is rarely confined to a single cell type, contributing ambiguity to the interpretation of results from broadly applied manipulations. To obtain unambiguous information about retinal processing, it is desirable to have strategies for further restricting transgene expression to a few or even to a single cell type. We employed an intersectional strategy based on a Cre/Flp double recombinase system to target amacrine and ganglion cell types in the inner retina. We analyzed expression patterns in seven Flp drivers and then created combinational mouse lines by selective cross breeding with Cre drivers. Breeding with Flp drivers can routinely remove labeling from more than 90% of the cells in Cre drivers, leading to only a handful cell types, typically 2–3, remaining in the intersection. Cre/Flp combinatorial mouse lines enabled us to identify and anatomically characterize retinal cell types with greater ease and demonstrated the feasibility of intersectional strategies in retinal research. In addition to the retina, we examined Flp expression in the lateral geniculate nucleus and superior colliculus. Our results establish a foundation for future application of intersectional strategies in the retina and retino-recipient regions. Frontiers Media S.A. 2018-08-22 /pmc/articles/PMC6113359/ /pubmed/30186122 http://dx.doi.org/10.3389/fncir.2018.00066 Text en Copyright © 2018 Jo, Xu, Deniz, Cherian, DeVries and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Jo, Andrew
Xu, Jian
Deniz, Sercan
Cherian, Suraj
DeVries, Steven H.
Zhu, Yongling
Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
title Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
title_full Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
title_fullStr Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
title_full_unstemmed Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
title_short Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
title_sort intersectional strategies for targeting amacrine and ganglion cell types in the mouse retina
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113359/
https://www.ncbi.nlm.nih.gov/pubmed/30186122
http://dx.doi.org/10.3389/fncir.2018.00066
work_keys_str_mv AT joandrew intersectionalstrategiesfortargetingamacrineandganglioncelltypesinthemouseretina
AT xujian intersectionalstrategiesfortargetingamacrineandganglioncelltypesinthemouseretina
AT denizsercan intersectionalstrategiesfortargetingamacrineandganglioncelltypesinthemouseretina
AT cheriansuraj intersectionalstrategiesfortargetingamacrineandganglioncelltypesinthemouseretina
AT devriesstevenh intersectionalstrategiesfortargetingamacrineandganglioncelltypesinthemouseretina
AT zhuyongling intersectionalstrategiesfortargetingamacrineandganglioncelltypesinthemouseretina