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Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina
The mammalian retina harbors over 100 different cell types. To understand how retinal circuits work, it is essential to systematically access each type. A widely used approach for achieving targeted transgene expression exploits promoter-driven Cre lines. However, Cre expression in a given transgeni...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113359/ https://www.ncbi.nlm.nih.gov/pubmed/30186122 http://dx.doi.org/10.3389/fncir.2018.00066 |
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author | Jo, Andrew Xu, Jian Deniz, Sercan Cherian, Suraj DeVries, Steven H. Zhu, Yongling |
author_facet | Jo, Andrew Xu, Jian Deniz, Sercan Cherian, Suraj DeVries, Steven H. Zhu, Yongling |
author_sort | Jo, Andrew |
collection | PubMed |
description | The mammalian retina harbors over 100 different cell types. To understand how retinal circuits work, it is essential to systematically access each type. A widely used approach for achieving targeted transgene expression exploits promoter-driven Cre lines. However, Cre expression in a given transgenic line in the retina and elsewhere in the brain is rarely confined to a single cell type, contributing ambiguity to the interpretation of results from broadly applied manipulations. To obtain unambiguous information about retinal processing, it is desirable to have strategies for further restricting transgene expression to a few or even to a single cell type. We employed an intersectional strategy based on a Cre/Flp double recombinase system to target amacrine and ganglion cell types in the inner retina. We analyzed expression patterns in seven Flp drivers and then created combinational mouse lines by selective cross breeding with Cre drivers. Breeding with Flp drivers can routinely remove labeling from more than 90% of the cells in Cre drivers, leading to only a handful cell types, typically 2–3, remaining in the intersection. Cre/Flp combinatorial mouse lines enabled us to identify and anatomically characterize retinal cell types with greater ease and demonstrated the feasibility of intersectional strategies in retinal research. In addition to the retina, we examined Flp expression in the lateral geniculate nucleus and superior colliculus. Our results establish a foundation for future application of intersectional strategies in the retina and retino-recipient regions. |
format | Online Article Text |
id | pubmed-6113359 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61133592018-09-05 Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina Jo, Andrew Xu, Jian Deniz, Sercan Cherian, Suraj DeVries, Steven H. Zhu, Yongling Front Neural Circuits Neuroscience The mammalian retina harbors over 100 different cell types. To understand how retinal circuits work, it is essential to systematically access each type. A widely used approach for achieving targeted transgene expression exploits promoter-driven Cre lines. However, Cre expression in a given transgenic line in the retina and elsewhere in the brain is rarely confined to a single cell type, contributing ambiguity to the interpretation of results from broadly applied manipulations. To obtain unambiguous information about retinal processing, it is desirable to have strategies for further restricting transgene expression to a few or even to a single cell type. We employed an intersectional strategy based on a Cre/Flp double recombinase system to target amacrine and ganglion cell types in the inner retina. We analyzed expression patterns in seven Flp drivers and then created combinational mouse lines by selective cross breeding with Cre drivers. Breeding with Flp drivers can routinely remove labeling from more than 90% of the cells in Cre drivers, leading to only a handful cell types, typically 2–3, remaining in the intersection. Cre/Flp combinatorial mouse lines enabled us to identify and anatomically characterize retinal cell types with greater ease and demonstrated the feasibility of intersectional strategies in retinal research. In addition to the retina, we examined Flp expression in the lateral geniculate nucleus and superior colliculus. Our results establish a foundation for future application of intersectional strategies in the retina and retino-recipient regions. Frontiers Media S.A. 2018-08-22 /pmc/articles/PMC6113359/ /pubmed/30186122 http://dx.doi.org/10.3389/fncir.2018.00066 Text en Copyright © 2018 Jo, Xu, Deniz, Cherian, DeVries and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Jo, Andrew Xu, Jian Deniz, Sercan Cherian, Suraj DeVries, Steven H. Zhu, Yongling Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina |
title | Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina |
title_full | Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina |
title_fullStr | Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina |
title_full_unstemmed | Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina |
title_short | Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina |
title_sort | intersectional strategies for targeting amacrine and ganglion cell types in the mouse retina |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113359/ https://www.ncbi.nlm.nih.gov/pubmed/30186122 http://dx.doi.org/10.3389/fncir.2018.00066 |
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