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Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells
BACKGROUND: Chemotherapy is a primary approach in cancer treatment after routine surgery. However, chemo-resistance tends to occur with chemotherapy in clinic, resulting in poor prognosis and recurrence. Nowadays, Chinese medicine may shed light on design of new therapeutic modes to overcome chemo-r...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114245/ https://www.ncbi.nlm.nih.gov/pubmed/30181769 http://dx.doi.org/10.1186/s13020-018-0203-z |
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author | Zhong, Zhangfeng Yu, Haibing Wang, Shengpeng Wang, Yitao Cui, Liao |
author_facet | Zhong, Zhangfeng Yu, Haibing Wang, Shengpeng Wang, Yitao Cui, Liao |
author_sort | Zhong, Zhangfeng |
collection | PubMed |
description | BACKGROUND: Chemotherapy is a primary approach in cancer treatment after routine surgery. However, chemo-resistance tends to occur with chemotherapy in clinic, resulting in poor prognosis and recurrence. Nowadays, Chinese medicine may shed light on design of new therapeutic modes to overcome chemo-resistance. Although Rhizoma Curcumae possesses anti-cancer activities in various types of cancers, the effects and underlying mechanisms of its bioactive components against chemo-resistance are not clear. Therefore, the present study aims to explore the potential effects of Rhizoma Curcumae on doxorubicin-resistant breast cancer cells. METHODS: The expression and function of ABC transporters in doxorubicin-resistant MCF-7 breast cancer cells were measured by western blotting and flow cytometry. Cell viability was detected using MTT assay. The combination index was analyzed using the CalcuSyn program (Biosoft, Ferguson, MO), based on the Chou–Talalay method. RESULTS: In our present study, P-gp was overexpressed at protein level in doxorubicin-resistant MCF-7 cell line, but short of MRP1 and BCRP1. Essential oil of Rhizoma Curcumae and the main bioactive components were assessed on doxorubicin-resistant MCF-7 cell line. We found that the essential oil and furanodiene both display powerful inhibitory effects on cell viability, but neither of these is the specific inhibitor of ABC transporters. Moreover, furanodiene fails to enhance the efficacy of doxorubicin to improve multidrug resistance. CONCLUSION: Overall, our findings fill the gaps of the researches on chemo-resistance improvement of Rhizoma Curcumae and are also beneficial for Rhizoma Curcumae being developed as a promising natural product for cancer adjuvant therapy in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0203-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6114245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61142452018-09-04 Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells Zhong, Zhangfeng Yu, Haibing Wang, Shengpeng Wang, Yitao Cui, Liao Chin Med Research BACKGROUND: Chemotherapy is a primary approach in cancer treatment after routine surgery. However, chemo-resistance tends to occur with chemotherapy in clinic, resulting in poor prognosis and recurrence. Nowadays, Chinese medicine may shed light on design of new therapeutic modes to overcome chemo-resistance. Although Rhizoma Curcumae possesses anti-cancer activities in various types of cancers, the effects and underlying mechanisms of its bioactive components against chemo-resistance are not clear. Therefore, the present study aims to explore the potential effects of Rhizoma Curcumae on doxorubicin-resistant breast cancer cells. METHODS: The expression and function of ABC transporters in doxorubicin-resistant MCF-7 breast cancer cells were measured by western blotting and flow cytometry. Cell viability was detected using MTT assay. The combination index was analyzed using the CalcuSyn program (Biosoft, Ferguson, MO), based on the Chou–Talalay method. RESULTS: In our present study, P-gp was overexpressed at protein level in doxorubicin-resistant MCF-7 cell line, but short of MRP1 and BCRP1. Essential oil of Rhizoma Curcumae and the main bioactive components were assessed on doxorubicin-resistant MCF-7 cell line. We found that the essential oil and furanodiene both display powerful inhibitory effects on cell viability, but neither of these is the specific inhibitor of ABC transporters. Moreover, furanodiene fails to enhance the efficacy of doxorubicin to improve multidrug resistance. CONCLUSION: Overall, our findings fill the gaps of the researches on chemo-resistance improvement of Rhizoma Curcumae and are also beneficial for Rhizoma Curcumae being developed as a promising natural product for cancer adjuvant therapy in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0203-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-29 /pmc/articles/PMC6114245/ /pubmed/30181769 http://dx.doi.org/10.1186/s13020-018-0203-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhong, Zhangfeng Yu, Haibing Wang, Shengpeng Wang, Yitao Cui, Liao Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells |
title | Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells |
title_full | Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells |
title_fullStr | Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells |
title_full_unstemmed | Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells |
title_short | Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells |
title_sort | anti-cancer effects of rhizoma curcumae against doxorubicin-resistant breast cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114245/ https://www.ncbi.nlm.nih.gov/pubmed/30181769 http://dx.doi.org/10.1186/s13020-018-0203-z |
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