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Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse
The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not re...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114792/ https://www.ncbi.nlm.nih.gov/pubmed/30157221 http://dx.doi.org/10.1371/journal.pone.0202804 |
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author | Sylvestre, Tatiane Fernanda Cavalcante, Ricardo de Souza da Silva, Julhiany de Fátima Paniago, Anamaria Mello Miranda Weber, Simone Schneider Pauletti, Bianca Alves de Carvalho, Lídia Raquel dos Santos, Lucilene Delazari Mendes, Rinaldo Poncio |
author_facet | Sylvestre, Tatiane Fernanda Cavalcante, Ricardo de Souza da Silva, Julhiany de Fátima Paniago, Anamaria Mello Miranda Weber, Simone Schneider Pauletti, Bianca Alves de Carvalho, Lídia Raquel dos Santos, Lucilene Delazari Mendes, Rinaldo Poncio |
author_sort | Sylvestre, Tatiane Fernanda |
collection | PubMed |
description | The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings. |
format | Online Article Text |
id | pubmed-6114792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61147922018-09-17 Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse Sylvestre, Tatiane Fernanda Cavalcante, Ricardo de Souza da Silva, Julhiany de Fátima Paniago, Anamaria Mello Miranda Weber, Simone Schneider Pauletti, Bianca Alves de Carvalho, Lídia Raquel dos Santos, Lucilene Delazari Mendes, Rinaldo Poncio PLoS One Research Article The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings. Public Library of Science 2018-08-29 /pmc/articles/PMC6114792/ /pubmed/30157221 http://dx.doi.org/10.1371/journal.pone.0202804 Text en © 2018 Sylvestre et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sylvestre, Tatiane Fernanda Cavalcante, Ricardo de Souza da Silva, Julhiany de Fátima Paniago, Anamaria Mello Miranda Weber, Simone Schneider Pauletti, Bianca Alves de Carvalho, Lídia Raquel dos Santos, Lucilene Delazari Mendes, Rinaldo Poncio Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse |
title | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse |
title_full | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse |
title_fullStr | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse |
title_full_unstemmed | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse |
title_short | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse |
title_sort | serological proteomic biomarkers to identify paracoccidioides species and risk of relapse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114792/ https://www.ncbi.nlm.nih.gov/pubmed/30157221 http://dx.doi.org/10.1371/journal.pone.0202804 |
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