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Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro

BACKGROUND: Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway. METHODS...

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Autores principales: Shen, Wei, Luo, Hui, Xu, Liangliang, Wu, Zhifang, Chen, Hongtai, Liu, Yamei, Yu, Lijuan, Hu, Liuchao, Wang, Bin, Luo, Yiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114862/
https://www.ncbi.nlm.nih.gov/pubmed/30181770
http://dx.doi.org/10.1186/s13020-018-0200-2
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author Shen, Wei
Luo, Hui
Xu, Liangliang
Wu, Zhifang
Chen, Hongtai
Liu, Yamei
Yu, Lijuan
Hu, Liuchao
Wang, Bin
Luo, Yiwen
author_facet Shen, Wei
Luo, Hui
Xu, Liangliang
Wu, Zhifang
Chen, Hongtai
Liu, Yamei
Yu, Lijuan
Hu, Liuchao
Wang, Bin
Luo, Yiwen
author_sort Shen, Wei
collection PubMed
description BACKGROUND: Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway. METHODS: BHD was extracted by petroleum, and its composition was analysed. Cell viability in the presence of various concentrations of BHD for 24, 48 and 72 h was measured using a Cell Counting Kit-8 assay. Transwell assays and wound healing assays were used to observe the migration ability of BMSCs. Lentiviral vectors were used to knock down Wnt5a. Polymerase chain reaction and Western blot analyses were used to further compare Wnt5a signalling components at the mRNA and protein levels between groups. RESULTS: BHD treatment groups showed increased migration ability and Wnt5a expression. Knocking down Wnt5a using a lentivirus significantly inhibited the effects of BHD, which implies that BHD promotes BMSC migration ability through activation of Wnt5a. CONCLUSIONS: BHD can enhance BMSC migration, possibly by activating Wnt5a signalling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0200-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-61148622018-09-04 Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro Shen, Wei Luo, Hui Xu, Liangliang Wu, Zhifang Chen, Hongtai Liu, Yamei Yu, Lijuan Hu, Liuchao Wang, Bin Luo, Yiwen Chin Med Research BACKGROUND: Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway. METHODS: BHD was extracted by petroleum, and its composition was analysed. Cell viability in the presence of various concentrations of BHD for 24, 48 and 72 h was measured using a Cell Counting Kit-8 assay. Transwell assays and wound healing assays were used to observe the migration ability of BMSCs. Lentiviral vectors were used to knock down Wnt5a. Polymerase chain reaction and Western blot analyses were used to further compare Wnt5a signalling components at the mRNA and protein levels between groups. RESULTS: BHD treatment groups showed increased migration ability and Wnt5a expression. Knocking down Wnt5a using a lentivirus significantly inhibited the effects of BHD, which implies that BHD promotes BMSC migration ability through activation of Wnt5a. CONCLUSIONS: BHD can enhance BMSC migration, possibly by activating Wnt5a signalling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0200-2) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-29 /pmc/articles/PMC6114862/ /pubmed/30181770 http://dx.doi.org/10.1186/s13020-018-0200-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Shen, Wei
Luo, Hui
Xu, Liangliang
Wu, Zhifang
Chen, Hongtai
Liu, Yamei
Yu, Lijuan
Hu, Liuchao
Wang, Bin
Luo, Yiwen
Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
title Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
title_full Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
title_fullStr Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
title_full_unstemmed Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
title_short Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
title_sort wnt5a mediates the effects of bushen huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114862/
https://www.ncbi.nlm.nih.gov/pubmed/30181770
http://dx.doi.org/10.1186/s13020-018-0200-2
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