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Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration

PURPOSE: To identify disease-specific cytokine profile differences in the aqueous humor (AH) (other than the vascular endothelial growth factor) between patients with dry and treated wet age-related macular degeneration (AMD) and healthy controls. METHODS: This retrospective study drew on a case-ser...

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Autores principales: Spindler, Jan, Zandi, Souska, Pfister, Isabel B., Gerhardt, Christin, Garweg, Justus G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114931/
https://www.ncbi.nlm.nih.gov/pubmed/30157273
http://dx.doi.org/10.1371/journal.pone.0203337
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author Spindler, Jan
Zandi, Souska
Pfister, Isabel B.
Gerhardt, Christin
Garweg, Justus G.
author_facet Spindler, Jan
Zandi, Souska
Pfister, Isabel B.
Gerhardt, Christin
Garweg, Justus G.
author_sort Spindler, Jan
collection PubMed
description PURPOSE: To identify disease-specific cytokine profile differences in the aqueous humor (AH) (other than the vascular endothelial growth factor) between patients with dry and treated wet age-related macular degeneration (AMD) and healthy controls. METHODS: This retrospective study drew on a case-series of patients diagnosed with dry AMD (n = 25) and treated wet AMD (n = 19), as well as on healthy controls (no systemic therapy; n = 20) undergoing phacoemulsification or vitrectomy. Samples of AH and serum were collected in parallel at the beginning of surgery. The levels of 43 cytokines were simultaneously determined using the Bio-Plex® multiplex beads system. Differences between the three groups were statistically compared using the Kruskal-Wallis H-Test after applying the Bonferroni correction for multiple comparisons (p<0.0012). RESULTS: The concentrations of three cytokines were elevated in the AH of patients with dry AMD (CXCL6; p = 0.00067) and treated wet AMD (CXCL5, CXCL6, MIG/XCXL; all p<0.001) relative to those in the healthy controls. No other differences between the three groups were identified. The AH levels of seven cytokines (16%), including CXCL6, ranged below the lower limit of quantitation of the assay. Without the correction for multiple comparisons (p<0.05), the levels of 31 of the 43 cytokines in the AH of patients with AMD would have differed significantly from those in the control. The systemic cytokine profiles (serum) were similar in all three groups. CONCLUSIONS: No systematic differences in the AH cytokine environment were identified between patients with dry AMD and those with treated wet AMD. This finding might indicate that AMD is either the result of a persistent imbalance in the physiological tissue milieu, or that the localized process induces no significant change in the cytokine environment of the anterior ocular segment.
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spelling pubmed-61149312018-09-17 Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration Spindler, Jan Zandi, Souska Pfister, Isabel B. Gerhardt, Christin Garweg, Justus G. PLoS One Research Article PURPOSE: To identify disease-specific cytokine profile differences in the aqueous humor (AH) (other than the vascular endothelial growth factor) between patients with dry and treated wet age-related macular degeneration (AMD) and healthy controls. METHODS: This retrospective study drew on a case-series of patients diagnosed with dry AMD (n = 25) and treated wet AMD (n = 19), as well as on healthy controls (no systemic therapy; n = 20) undergoing phacoemulsification or vitrectomy. Samples of AH and serum were collected in parallel at the beginning of surgery. The levels of 43 cytokines were simultaneously determined using the Bio-Plex® multiplex beads system. Differences between the three groups were statistically compared using the Kruskal-Wallis H-Test after applying the Bonferroni correction for multiple comparisons (p<0.0012). RESULTS: The concentrations of three cytokines were elevated in the AH of patients with dry AMD (CXCL6; p = 0.00067) and treated wet AMD (CXCL5, CXCL6, MIG/XCXL; all p<0.001) relative to those in the healthy controls. No other differences between the three groups were identified. The AH levels of seven cytokines (16%), including CXCL6, ranged below the lower limit of quantitation of the assay. Without the correction for multiple comparisons (p<0.05), the levels of 31 of the 43 cytokines in the AH of patients with AMD would have differed significantly from those in the control. The systemic cytokine profiles (serum) were similar in all three groups. CONCLUSIONS: No systematic differences in the AH cytokine environment were identified between patients with dry AMD and those with treated wet AMD. This finding might indicate that AMD is either the result of a persistent imbalance in the physiological tissue milieu, or that the localized process induces no significant change in the cytokine environment of the anterior ocular segment. Public Library of Science 2018-08-29 /pmc/articles/PMC6114931/ /pubmed/30157273 http://dx.doi.org/10.1371/journal.pone.0203337 Text en © 2018 Spindler et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Spindler, Jan
Zandi, Souska
Pfister, Isabel B.
Gerhardt, Christin
Garweg, Justus G.
Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
title Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
title_full Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
title_fullStr Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
title_full_unstemmed Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
title_short Cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
title_sort cytokine profiles in the aqueous humor and serum of patients with dry and treated wet age-related macular degeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114931/
https://www.ncbi.nlm.nih.gov/pubmed/30157273
http://dx.doi.org/10.1371/journal.pone.0203337
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