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Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach

We conducted an in silico analysis to search for important genes in the pathogenesis of Caseous Lymphadenitis (CL), with prospects for use in formulating effective vaccines against this disease. For this, we performed a survey of proteins expressed by Corynebacterium pseudotuberculosis, using protei...

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Autores principales: SOBRINHO SANTOS, Eliane Macedo, ALMEIDA, Anna Christina, SANTOS, Hércules Otacílio, CANGUSSU, Alex Sander Rodrigues, ALMEIDA, Deborah Aires, COSTA, Kattyanne Souza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115270/
https://www.ncbi.nlm.nih.gov/pubmed/29937460
http://dx.doi.org/10.1292/jvms.16-0581
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author SOBRINHO SANTOS, Eliane Macedo
ALMEIDA, Anna Christina
SANTOS, Hércules Otacílio
CANGUSSU, Alex Sander Rodrigues
ALMEIDA, Deborah Aires
COSTA, Kattyanne Souza
author_facet SOBRINHO SANTOS, Eliane Macedo
ALMEIDA, Anna Christina
SANTOS, Hércules Otacílio
CANGUSSU, Alex Sander Rodrigues
ALMEIDA, Deborah Aires
COSTA, Kattyanne Souza
author_sort SOBRINHO SANTOS, Eliane Macedo
collection PubMed
description We conducted an in silico analysis to search for important genes in the pathogenesis of Caseous Lymphadenitis (CL), with prospects for use in formulating effective vaccines against this disease. For this, we performed a survey of proteins expressed by Corynebacterium pseudotuberculosis, using protein sequences collected from the NCBI GenPept database and the keywords “caseous lymphadenitis” and “Corynebacterium pseudotuberculosis” and “goats”. A network was developed using the STRING 10 database, with a confidence score of 0.900. For every gene interaction identified, we summed the interaction score of each gene, generating a combined association score to obtain a single score named weighted number of links (WNL). Genes with the highest WNL were named “leader genes”. Ontological analysis was extracted from the STRING database through Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A search in the GenPept database revealed 2,124 proteins. By using and plotting with STRING 10, we then developed an in silico network model comprised of 1,243 genes/proteins interconnecting through 3,330 interactions. The highest WNL values were identified in the rplB gene, which was named the leader gene. Our ontological analysis shows that this protein acts effectively mainly on Metabolic pathways and Biosynthesis of secondary metabolites. In conclusion, the in silico analyses showed that rplB has good potential for vaccine development. However, functional assays are needed to make sure that this protein can potentially induce both humoral and cellular immune responses against C. pseudotuberculosis in goats.
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spelling pubmed-61152702018-09-24 Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach SOBRINHO SANTOS, Eliane Macedo ALMEIDA, Anna Christina SANTOS, Hércules Otacílio CANGUSSU, Alex Sander Rodrigues ALMEIDA, Deborah Aires COSTA, Kattyanne Souza J Vet Med Sci Immunology We conducted an in silico analysis to search for important genes in the pathogenesis of Caseous Lymphadenitis (CL), with prospects for use in formulating effective vaccines against this disease. For this, we performed a survey of proteins expressed by Corynebacterium pseudotuberculosis, using protein sequences collected from the NCBI GenPept database and the keywords “caseous lymphadenitis” and “Corynebacterium pseudotuberculosis” and “goats”. A network was developed using the STRING 10 database, with a confidence score of 0.900. For every gene interaction identified, we summed the interaction score of each gene, generating a combined association score to obtain a single score named weighted number of links (WNL). Genes with the highest WNL were named “leader genes”. Ontological analysis was extracted from the STRING database through Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A search in the GenPept database revealed 2,124 proteins. By using and plotting with STRING 10, we then developed an in silico network model comprised of 1,243 genes/proteins interconnecting through 3,330 interactions. The highest WNL values were identified in the rplB gene, which was named the leader gene. Our ontological analysis shows that this protein acts effectively mainly on Metabolic pathways and Biosynthesis of secondary metabolites. In conclusion, the in silico analyses showed that rplB has good potential for vaccine development. However, functional assays are needed to make sure that this protein can potentially induce both humoral and cellular immune responses against C. pseudotuberculosis in goats. The Japanese Society of Veterinary Science 2018-06-25 2018-08 /pmc/articles/PMC6115270/ /pubmed/29937460 http://dx.doi.org/10.1292/jvms.16-0581 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Immunology
SOBRINHO SANTOS, Eliane Macedo
ALMEIDA, Anna Christina
SANTOS, Hércules Otacílio
CANGUSSU, Alex Sander Rodrigues
ALMEIDA, Deborah Aires
COSTA, Kattyanne Souza
Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
title Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
title_full Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
title_fullStr Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
title_full_unstemmed Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
title_short Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
title_sort leader gene of corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115270/
https://www.ncbi.nlm.nih.gov/pubmed/29937460
http://dx.doi.org/10.1292/jvms.16-0581
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