Cargando…

OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate

The response of plant root development to nutrient deficiencies is critical for crop production. Auxin, nitric oxide (NO), and strigolactones (SLs) are important regulators of root growth under low-nitrogen and -phosphate (LN and LP) conditions. Polar auxin transport in plants, which is mainly depen...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Huwei, Tao, Jinyuan, Bi, Yang, Hou, Mengmeng, Lou, Jiajing, Chen, Xinni, Zhang, Xuhong, Luo, Le, Xie, Xiaonan, Yoneyama, Koichi, Zhao, Quanzhi, Xu, Guohua, Zhang, Yali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115472/
https://www.ncbi.nlm.nih.gov/pubmed/30158652
http://dx.doi.org/10.1038/s41598-018-29784-x
_version_ 1783351392055853056
author Sun, Huwei
Tao, Jinyuan
Bi, Yang
Hou, Mengmeng
Lou, Jiajing
Chen, Xinni
Zhang, Xuhong
Luo, Le
Xie, Xiaonan
Yoneyama, Koichi
Zhao, Quanzhi
Xu, Guohua
Zhang, Yali
author_facet Sun, Huwei
Tao, Jinyuan
Bi, Yang
Hou, Mengmeng
Lou, Jiajing
Chen, Xinni
Zhang, Xuhong
Luo, Le
Xie, Xiaonan
Yoneyama, Koichi
Zhao, Quanzhi
Xu, Guohua
Zhang, Yali
author_sort Sun, Huwei
collection PubMed
description The response of plant root development to nutrient deficiencies is critical for crop production. Auxin, nitric oxide (NO), and strigolactones (SLs) are important regulators of root growth under low-nitrogen and -phosphate (LN and LP) conditions. Polar auxin transport in plants, which is mainly dependent on auxin efflux protein PINs, creates local auxin maxima to form the basis for root initiation and elongation; however, the PIN genes that play an important role in LN- and LP-modulated root growth remain unclear. qRT-PCR analysis of OsPIN family genes showed that the expression of OsPIN1b is most abundant in root tip and is significantly downregulated by LN, LP, sodium nitroprusside (SNP, NO donor), and GR24 (analogue of SLs) treatments. Seminal roots in ospin1b mutants were shorter than those of the wild type; and the seminal root, [(3)H]IAA transport, and IAA concentration responses to LN, LP, SNP, and GR24 application were attenuated in ospin1b-1 mutants. pCYCB1;1::GUS expression was upregulated by LN, LP, SNP, and GR24 treatments in wild type, but not in the ospin1b-1 mutant, suggesting that OsPIN1b is involved in auxin transport and acts as a downstream mediator of NO and SLs to induce meristem activity in root tip in rice under LN and LP.
format Online
Article
Text
id pubmed-6115472
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61154722018-09-05 OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate Sun, Huwei Tao, Jinyuan Bi, Yang Hou, Mengmeng Lou, Jiajing Chen, Xinni Zhang, Xuhong Luo, Le Xie, Xiaonan Yoneyama, Koichi Zhao, Quanzhi Xu, Guohua Zhang, Yali Sci Rep Article The response of plant root development to nutrient deficiencies is critical for crop production. Auxin, nitric oxide (NO), and strigolactones (SLs) are important regulators of root growth under low-nitrogen and -phosphate (LN and LP) conditions. Polar auxin transport in plants, which is mainly dependent on auxin efflux protein PINs, creates local auxin maxima to form the basis for root initiation and elongation; however, the PIN genes that play an important role in LN- and LP-modulated root growth remain unclear. qRT-PCR analysis of OsPIN family genes showed that the expression of OsPIN1b is most abundant in root tip and is significantly downregulated by LN, LP, sodium nitroprusside (SNP, NO donor), and GR24 (analogue of SLs) treatments. Seminal roots in ospin1b mutants were shorter than those of the wild type; and the seminal root, [(3)H]IAA transport, and IAA concentration responses to LN, LP, SNP, and GR24 application were attenuated in ospin1b-1 mutants. pCYCB1;1::GUS expression was upregulated by LN, LP, SNP, and GR24 treatments in wild type, but not in the ospin1b-1 mutant, suggesting that OsPIN1b is involved in auxin transport and acts as a downstream mediator of NO and SLs to induce meristem activity in root tip in rice under LN and LP. Nature Publishing Group UK 2018-08-29 /pmc/articles/PMC6115472/ /pubmed/30158652 http://dx.doi.org/10.1038/s41598-018-29784-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sun, Huwei
Tao, Jinyuan
Bi, Yang
Hou, Mengmeng
Lou, Jiajing
Chen, Xinni
Zhang, Xuhong
Luo, Le
Xie, Xiaonan
Yoneyama, Koichi
Zhao, Quanzhi
Xu, Guohua
Zhang, Yali
OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate
title OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate
title_full OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate
title_fullStr OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate
title_full_unstemmed OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate
title_short OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate
title_sort ospin1b is involved in rice seminal root elongation by regulating root apical meristem activity in response to low nitrogen and phosphate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115472/
https://www.ncbi.nlm.nih.gov/pubmed/30158652
http://dx.doi.org/10.1038/s41598-018-29784-x
work_keys_str_mv AT sunhuwei ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT taojinyuan ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT biyang ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT houmengmeng ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT loujiajing ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT chenxinni ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT zhangxuhong ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT luole ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT xiexiaonan ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT yoneyamakoichi ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT zhaoquanzhi ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT xuguohua ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate
AT zhangyali ospin1bisinvolvedinriceseminalrootelongationbyregulatingrootapicalmeristemactivityinresponsetolownitrogenandphosphate