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Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease

The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of Phyllanthus niruri (50% ME of P. niruri) has alleviated NAFLD in Sprague–Dawley rats. This study aimed t...

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Autores principales: Al Zarzour, Raghdaa Hamdan, Alshawsh, Mohammed A., Asif, Muhammad, Al-Mansoub, Majed Ahmed, Mohamed, Zahurin, Ahmad, Mariam, Abdul Majid, Amin Malik Shah, Asmawi, Mohd. Zaini, Kaur, Gurjeet, Al-dualimi, Dhamraa Waleed, Yam, Mun Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115813/
https://www.ncbi.nlm.nih.gov/pubmed/30096951
http://dx.doi.org/10.3390/nu10081057
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author Al Zarzour, Raghdaa Hamdan
Alshawsh, Mohammed A.
Asif, Muhammad
Al-Mansoub, Majed Ahmed
Mohamed, Zahurin
Ahmad, Mariam
Abdul Majid, Amin Malik Shah
Asmawi, Mohd. Zaini
Kaur, Gurjeet
Al-dualimi, Dhamraa Waleed
Yam, Mun Fei
author_facet Al Zarzour, Raghdaa Hamdan
Alshawsh, Mohammed A.
Asif, Muhammad
Al-Mansoub, Majed Ahmed
Mohamed, Zahurin
Ahmad, Mariam
Abdul Majid, Amin Malik Shah
Asmawi, Mohd. Zaini
Kaur, Gurjeet
Al-dualimi, Dhamraa Waleed
Yam, Mun Fei
author_sort Al Zarzour, Raghdaa Hamdan
collection PubMed
description The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of Phyllanthus niruri (50% ME of P. niruri) has alleviated NAFLD in Sprague–Dawley rats. This study aimed to assess the molecular mechanisms of action, and to further evaluate the antiangiogenic effect of this extract. NAFLD was induced by eight weeks of high-fat diet, and treatment was applied for four weeks. Antiangiogenic activity was assessed by aortic ring assay and by in vitro tests. Our findings demonstrated that the therapeutic effects of 50% ME among NAFLD rats, were associated with a significant increase in serum adiponectin, reduction in the serum levels of RBP4, vaspin, progranulin, TNF-α, IL-6, and significant downregulation of the hepatic gene expression of PPARγ, SLC10A2, and Collα1. Concomitantly, 50% ME of P. niruri has exhibited a potent antiangiogenic activity on ring assay, cell migration, vascular endothelial growth factor (VEGF), and tube formation, without any cytotoxic effect. Together, our findings revealed that the protective effects of P. niruri against NAFLD might be attributed to its antiangiogenic effect, as well as to the regulation of adipocytokines and reducing the expression of adipogenic genes.
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spelling pubmed-61158132018-09-04 Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease Al Zarzour, Raghdaa Hamdan Alshawsh, Mohammed A. Asif, Muhammad Al-Mansoub, Majed Ahmed Mohamed, Zahurin Ahmad, Mariam Abdul Majid, Amin Malik Shah Asmawi, Mohd. Zaini Kaur, Gurjeet Al-dualimi, Dhamraa Waleed Yam, Mun Fei Nutrients Article The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of Phyllanthus niruri (50% ME of P. niruri) has alleviated NAFLD in Sprague–Dawley rats. This study aimed to assess the molecular mechanisms of action, and to further evaluate the antiangiogenic effect of this extract. NAFLD was induced by eight weeks of high-fat diet, and treatment was applied for four weeks. Antiangiogenic activity was assessed by aortic ring assay and by in vitro tests. Our findings demonstrated that the therapeutic effects of 50% ME among NAFLD rats, were associated with a significant increase in serum adiponectin, reduction in the serum levels of RBP4, vaspin, progranulin, TNF-α, IL-6, and significant downregulation of the hepatic gene expression of PPARγ, SLC10A2, and Collα1. Concomitantly, 50% ME of P. niruri has exhibited a potent antiangiogenic activity on ring assay, cell migration, vascular endothelial growth factor (VEGF), and tube formation, without any cytotoxic effect. Together, our findings revealed that the protective effects of P. niruri against NAFLD might be attributed to its antiangiogenic effect, as well as to the regulation of adipocytokines and reducing the expression of adipogenic genes. MDPI 2018-08-09 /pmc/articles/PMC6115813/ /pubmed/30096951 http://dx.doi.org/10.3390/nu10081057 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al Zarzour, Raghdaa Hamdan
Alshawsh, Mohammed A.
Asif, Muhammad
Al-Mansoub, Majed Ahmed
Mohamed, Zahurin
Ahmad, Mariam
Abdul Majid, Amin Malik Shah
Asmawi, Mohd. Zaini
Kaur, Gurjeet
Al-dualimi, Dhamraa Waleed
Yam, Mun Fei
Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_full Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_fullStr Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_full_unstemmed Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_short Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_sort adipocytokine regulation and antiangiogenic activity underlie the molecular mechanisms of therapeutic effects of phyllanthus niruri against non-alcoholic fatty liver disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115813/
https://www.ncbi.nlm.nih.gov/pubmed/30096951
http://dx.doi.org/10.3390/nu10081057
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