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Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14
Ensifer (Sinorhizobium) sp. M14 is an efficient arsenic-oxidizing bacterium (AOB) that displays high resistance to numerous metals and various stressors. Here, we report the draft genome sequence and genome-guided characterization of Ensifer sp. M14, and we describe a pilot-scale installation applyi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115938/ https://www.ncbi.nlm.nih.gov/pubmed/30060533 http://dx.doi.org/10.3390/genes9080379 |
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author | diCenzo, George C Debiec, Klaudia Krzysztoforski, Jan Uhrynowski, Witold Mengoni, Alessio Fagorzi, Camilla Gorecki, Adrian Dziewit, Lukasz Bajda, Tomasz Rzepa, Grzegorz Drewniak, Lukasz |
author_facet | diCenzo, George C Debiec, Klaudia Krzysztoforski, Jan Uhrynowski, Witold Mengoni, Alessio Fagorzi, Camilla Gorecki, Adrian Dziewit, Lukasz Bajda, Tomasz Rzepa, Grzegorz Drewniak, Lukasz |
author_sort | diCenzo, George C |
collection | PubMed |
description | Ensifer (Sinorhizobium) sp. M14 is an efficient arsenic-oxidizing bacterium (AOB) that displays high resistance to numerous metals and various stressors. Here, we report the draft genome sequence and genome-guided characterization of Ensifer sp. M14, and we describe a pilot-scale installation applying the M14 strain for remediation of arsenic-contaminated waters. The M14 genome contains 6874 protein coding sequences, including hundreds not found in related strains. Nearly all unique genes that are associated with metal resistance and arsenic oxidation are localized within the pSinA and pSinB megaplasmids. Comparative genomics revealed that multiple copies of high-affinity phosphate transport systems are common in AOBs, possibly as an As-resistance mechanism. Genome and antibiotic sensitivity analyses further suggested that the use of Ensifer sp. M14 in biotechnology does not pose serious biosafety risks. Therefore, a novel two-stage installation for remediation of arsenic-contaminated waters was developed. It consists of a microbiological module, where M14 oxidizes As(III) to As(V) ion, followed by an adsorption module for As(V) removal using granulated bog iron ores. During a 40-day pilot-scale test in an abandoned gold mine in Zloty Stok (Poland), water leaving the microbiological module generally contained trace amounts of As(III), and dramatic decreases in total arsenic concentrations were observed after passage through the adsorption module. These results demonstrate the usefulness of Ensifer sp. M14 in arsenic removal performed in environmental settings. |
format | Online Article Text |
id | pubmed-6115938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61159382018-08-31 Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 diCenzo, George C Debiec, Klaudia Krzysztoforski, Jan Uhrynowski, Witold Mengoni, Alessio Fagorzi, Camilla Gorecki, Adrian Dziewit, Lukasz Bajda, Tomasz Rzepa, Grzegorz Drewniak, Lukasz Genes (Basel) Article Ensifer (Sinorhizobium) sp. M14 is an efficient arsenic-oxidizing bacterium (AOB) that displays high resistance to numerous metals and various stressors. Here, we report the draft genome sequence and genome-guided characterization of Ensifer sp. M14, and we describe a pilot-scale installation applying the M14 strain for remediation of arsenic-contaminated waters. The M14 genome contains 6874 protein coding sequences, including hundreds not found in related strains. Nearly all unique genes that are associated with metal resistance and arsenic oxidation are localized within the pSinA and pSinB megaplasmids. Comparative genomics revealed that multiple copies of high-affinity phosphate transport systems are common in AOBs, possibly as an As-resistance mechanism. Genome and antibiotic sensitivity analyses further suggested that the use of Ensifer sp. M14 in biotechnology does not pose serious biosafety risks. Therefore, a novel two-stage installation for remediation of arsenic-contaminated waters was developed. It consists of a microbiological module, where M14 oxidizes As(III) to As(V) ion, followed by an adsorption module for As(V) removal using granulated bog iron ores. During a 40-day pilot-scale test in an abandoned gold mine in Zloty Stok (Poland), water leaving the microbiological module generally contained trace amounts of As(III), and dramatic decreases in total arsenic concentrations were observed after passage through the adsorption module. These results demonstrate the usefulness of Ensifer sp. M14 in arsenic removal performed in environmental settings. MDPI 2018-07-27 /pmc/articles/PMC6115938/ /pubmed/30060533 http://dx.doi.org/10.3390/genes9080379 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article diCenzo, George C Debiec, Klaudia Krzysztoforski, Jan Uhrynowski, Witold Mengoni, Alessio Fagorzi, Camilla Gorecki, Adrian Dziewit, Lukasz Bajda, Tomasz Rzepa, Grzegorz Drewniak, Lukasz Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 |
title | Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 |
title_full | Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 |
title_fullStr | Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 |
title_full_unstemmed | Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 |
title_short | Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14 |
title_sort | genomic and biotechnological characterization of the heavy-metal resistant, arsenic-oxidizing bacterium ensifer sp. m14 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115938/ https://www.ncbi.nlm.nih.gov/pubmed/30060533 http://dx.doi.org/10.3390/genes9080379 |
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