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Blueprints for Biosensors: Design, Limitations, and Applications
Biosensors are enabling major advances in the field of analytics that are both facilitating and being facilitated by advances in synthetic biology. The ability of biosensors to rapidly and specifically detect a wide range of molecules makes them highly relevant to a range of industrial, medical, eco...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115959/ https://www.ncbi.nlm.nih.gov/pubmed/30050028 http://dx.doi.org/10.3390/genes9080375 |
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author | Carpenter, Alexander C. Paulsen, Ian T. Williams, Thomas C. |
author_facet | Carpenter, Alexander C. Paulsen, Ian T. Williams, Thomas C. |
author_sort | Carpenter, Alexander C. |
collection | PubMed |
description | Biosensors are enabling major advances in the field of analytics that are both facilitating and being facilitated by advances in synthetic biology. The ability of biosensors to rapidly and specifically detect a wide range of molecules makes them highly relevant to a range of industrial, medical, ecological, and scientific applications. Approaches to biosensor design are as diverse as their applications, with major biosensor classes including nucleic acids, proteins, and transcription factors. Each of these biosensor types has advantages and limitations based on the intended application, and the parameters that are required for optimal performance. Specifically, the choice of biosensor design must consider factors such as the ligand specificity, sensitivity, dynamic range, functional range, mode of output, time of activation, ease of use, and ease of engineering. This review discusses the rationale for designing the major classes of biosensor in the context of their limitations and assesses their suitability to different areas of biotechnological application. |
format | Online Article Text |
id | pubmed-6115959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61159592018-08-31 Blueprints for Biosensors: Design, Limitations, and Applications Carpenter, Alexander C. Paulsen, Ian T. Williams, Thomas C. Genes (Basel) Review Biosensors are enabling major advances in the field of analytics that are both facilitating and being facilitated by advances in synthetic biology. The ability of biosensors to rapidly and specifically detect a wide range of molecules makes them highly relevant to a range of industrial, medical, ecological, and scientific applications. Approaches to biosensor design are as diverse as their applications, with major biosensor classes including nucleic acids, proteins, and transcription factors. Each of these biosensor types has advantages and limitations based on the intended application, and the parameters that are required for optimal performance. Specifically, the choice of biosensor design must consider factors such as the ligand specificity, sensitivity, dynamic range, functional range, mode of output, time of activation, ease of use, and ease of engineering. This review discusses the rationale for designing the major classes of biosensor in the context of their limitations and assesses their suitability to different areas of biotechnological application. MDPI 2018-07-26 /pmc/articles/PMC6115959/ /pubmed/30050028 http://dx.doi.org/10.3390/genes9080375 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Carpenter, Alexander C. Paulsen, Ian T. Williams, Thomas C. Blueprints for Biosensors: Design, Limitations, and Applications |
title | Blueprints for Biosensors: Design, Limitations, and Applications |
title_full | Blueprints for Biosensors: Design, Limitations, and Applications |
title_fullStr | Blueprints for Biosensors: Design, Limitations, and Applications |
title_full_unstemmed | Blueprints for Biosensors: Design, Limitations, and Applications |
title_short | Blueprints for Biosensors: Design, Limitations, and Applications |
title_sort | blueprints for biosensors: design, limitations, and applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115959/ https://www.ncbi.nlm.nih.gov/pubmed/30050028 http://dx.doi.org/10.3390/genes9080375 |
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