Cargando…

Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles

Human Immunodeficiency Virus type-1 (HIV-1) acquires its lipid membrane from the plasma membrane of the infected cell from which it buds out. Previous studies have shown that the HIV-1 envelope is an environment of very low mobility, with the diffusion of incorporated proteins two orders of magnitud...

Descripción completa

Detalles Bibliográficos
Autores principales: Urbančič, Iztok, Brun, Juliane, Shrestha, Dilip, Waithe, Dominic, Eggeling, Christian, Chojnacki, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116177/
https://www.ncbi.nlm.nih.gov/pubmed/30096847
http://dx.doi.org/10.3390/v10080415
_version_ 1783351546161922048
author Urbančič, Iztok
Brun, Juliane
Shrestha, Dilip
Waithe, Dominic
Eggeling, Christian
Chojnacki, Jakub
author_facet Urbančič, Iztok
Brun, Juliane
Shrestha, Dilip
Waithe, Dominic
Eggeling, Christian
Chojnacki, Jakub
author_sort Urbančič, Iztok
collection PubMed
description Human Immunodeficiency Virus type-1 (HIV-1) acquires its lipid membrane from the plasma membrane of the infected cell from which it buds out. Previous studies have shown that the HIV-1 envelope is an environment of very low mobility, with the diffusion of incorporated proteins two orders of magnitude slower than in the plasma membrane. One of the reasons for this difference is thought to be the HIV-1 membrane composition that is characterised by a high degree of rigidity and lipid packing, which has, until now, been difficult to assess experimentally. To further refine the model of the molecular mobility on the HIV-1 surface, we herein investigated the relative importance of membrane composition and curvature in simplified model membrane systems, large unilamellar vesicles (LUVs) of different lipid compositions and sizes (0.1–1 µm), using super-resolution stimulated emission depletion (STED) microscopy-based fluorescence correlation spectroscopy (STED-FCS). Establishing an approach that is also applicable to measurements of molecule dynamics in virus-sized particles, we found, at least for the 0.1–1 µm sized vesicles, that the lipid composition and thus membrane rigidity, but not the curvature, play an important role in the decreased molecular mobility on the vesicles’ surface. This observation suggests that the composition of the envelope rather than the particle geometry contributes to the previously described low mobility of proteins on the HIV-1 surface. Our vesicle-based study thus provides further insight into the dynamic properties of the surface of individual HIV-1 particles, as well as paves the methodological way towards better characterisation of the properties and function of viral lipid envelopes in general.
format Online
Article
Text
id pubmed-6116177
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61161772018-08-31 Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles Urbančič, Iztok Brun, Juliane Shrestha, Dilip Waithe, Dominic Eggeling, Christian Chojnacki, Jakub Viruses Article Human Immunodeficiency Virus type-1 (HIV-1) acquires its lipid membrane from the plasma membrane of the infected cell from which it buds out. Previous studies have shown that the HIV-1 envelope is an environment of very low mobility, with the diffusion of incorporated proteins two orders of magnitude slower than in the plasma membrane. One of the reasons for this difference is thought to be the HIV-1 membrane composition that is characterised by a high degree of rigidity and lipid packing, which has, until now, been difficult to assess experimentally. To further refine the model of the molecular mobility on the HIV-1 surface, we herein investigated the relative importance of membrane composition and curvature in simplified model membrane systems, large unilamellar vesicles (LUVs) of different lipid compositions and sizes (0.1–1 µm), using super-resolution stimulated emission depletion (STED) microscopy-based fluorescence correlation spectroscopy (STED-FCS). Establishing an approach that is also applicable to measurements of molecule dynamics in virus-sized particles, we found, at least for the 0.1–1 µm sized vesicles, that the lipid composition and thus membrane rigidity, but not the curvature, play an important role in the decreased molecular mobility on the vesicles’ surface. This observation suggests that the composition of the envelope rather than the particle geometry contributes to the previously described low mobility of proteins on the HIV-1 surface. Our vesicle-based study thus provides further insight into the dynamic properties of the surface of individual HIV-1 particles, as well as paves the methodological way towards better characterisation of the properties and function of viral lipid envelopes in general. MDPI 2018-08-08 /pmc/articles/PMC6116177/ /pubmed/30096847 http://dx.doi.org/10.3390/v10080415 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Urbančič, Iztok
Brun, Juliane
Shrestha, Dilip
Waithe, Dominic
Eggeling, Christian
Chojnacki, Jakub
Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles
title Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles
title_full Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles
title_fullStr Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles
title_full_unstemmed Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles
title_short Lipid Composition but Not Curvature Is the Determinant Factor for the Low Molecular Mobility Observed on the Membrane of Virus-Like Vesicles
title_sort lipid composition but not curvature is the determinant factor for the low molecular mobility observed on the membrane of virus-like vesicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116177/
https://www.ncbi.nlm.nih.gov/pubmed/30096847
http://dx.doi.org/10.3390/v10080415
work_keys_str_mv AT urbanciciztok lipidcompositionbutnotcurvatureisthedeterminantfactorforthelowmolecularmobilityobservedonthemembraneofviruslikevesicles
AT brunjuliane lipidcompositionbutnotcurvatureisthedeterminantfactorforthelowmolecularmobilityobservedonthemembraneofviruslikevesicles
AT shresthadilip lipidcompositionbutnotcurvatureisthedeterminantfactorforthelowmolecularmobilityobservedonthemembraneofviruslikevesicles
AT waithedominic lipidcompositionbutnotcurvatureisthedeterminantfactorforthelowmolecularmobilityobservedonthemembraneofviruslikevesicles
AT eggelingchristian lipidcompositionbutnotcurvatureisthedeterminantfactorforthelowmolecularmobilityobservedonthemembraneofviruslikevesicles
AT chojnackijakub lipidcompositionbutnotcurvatureisthedeterminantfactorforthelowmolecularmobilityobservedonthemembraneofviruslikevesicles