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Facile Ag-Film Based Surface Enhanced Raman Spectroscopy Using DNA Molecular Switch for Ultra-Sensitive Mercury Ions Detection

Heavy metal pollution has long been the focus of attention because of its serious threat to human health and the environment. Surface enhanced Raman spectroscopy (SERS) has shown great potential for metal detection owing to many advantages, including, requiring fewer samples, its minimal damage to s...

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Detalles Bibliográficos
Autores principales: Liu, Xiujie, Liu, Mengmeng, Lu, Yudong, Wu, Changji, Xu, Yunchao, Lin, Duo, Lu, Dechan, Zhou, Ting, Feng, Shangyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116212/
https://www.ncbi.nlm.nih.gov/pubmed/30082591
http://dx.doi.org/10.3390/nano8080596
Descripción
Sumario:Heavy metal pollution has long been the focus of attention because of its serious threat to human health and the environment. Surface enhanced Raman spectroscopy (SERS) has shown great potential for metal detection owing to many advantages, including, requiring fewer samples, its minimal damage to specimen, and its high sensitivity. In this work, we proposed a simple and distinctive method, based on SERS, using facile silver film (Ag-film) combined with a DNA molecular switch, which allowed for the highly specific detection of heavy metal mercury ions (Hg(2+)). When in the presence of Hg(2+) ions, the signals from Raman probes attach to single-stranded DNA, which will be dramatically enhanced due to the specific structural change of DNA strands—resulting from the interaction between Hg(2+) ions and DNA bases. This SERS sensor could achieve an ultralow limit of detection (1.35 × 10(−15) M) for Hg(2+) detection. In addition, we applied this SERS sensor to detect Hg(2+) in real blood samples. The results suggested that this SERS platform could be a promising alternative tool for Hg(2+) detection in clinical, environmental, and food inspection.