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Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis

Leucine can affect intestinal protein expressions, and improve mucosal immune function. However, little study has been conducted to determine the change of protein component by leucine treatment in intestine epithelial cells. The present study was to cover the key proteins and cell pathways that cou...

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Autores principales: Mao, Xiangbing, Ren, Man, Chen, Daiwen, Yu, Bing, Che, Lianqiang, He, Jun, Luo, Junqiu, Luo, Yuheng, Wang, Jianping, Sun, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116325/
https://www.ncbi.nlm.nih.gov/pubmed/30175261
http://dx.doi.org/10.1016/j.aninu.2018.03.006
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author Mao, Xiangbing
Ren, Man
Chen, Daiwen
Yu, Bing
Che, Lianqiang
He, Jun
Luo, Junqiu
Luo, Yuheng
Wang, Jianping
Sun, Hui
author_facet Mao, Xiangbing
Ren, Man
Chen, Daiwen
Yu, Bing
Che, Lianqiang
He, Jun
Luo, Junqiu
Luo, Yuheng
Wang, Jianping
Sun, Hui
author_sort Mao, Xiangbing
collection PubMed
description Leucine can affect intestinal protein expressions, and improve mucosal immune function. However, little study has been conducted to determine the change of protein component by leucine treatment in intestine epithelial cells. The present study was to cover the key proteins and cell pathways that could be regulated by leucine treatment in porcine intestinal epithelial cell line (IPEC-J2) cells with the approach of proteome analysis. A total number of 3,211 proteins were identified in our approach by searching the database of Uniprot sus scrofa. Among identified proteins, there were 101 proteins expressed differently between control group and leucine group. Compared with the control group, there were 50 up-regulated proteins and 51 down-regulated proteins in leucine group. In these proteins, leucine treatment decreased the expression of some proteins including pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase, E3 ubiquitin ligase, cathepsin D, caspase 3 and caspase 6, and increased the levels of some proteins, such as some eukaryotic translation initiation factors, ubiquitin carboxyl-terminal hydrolase, DNA-related RNA polymerase II, urokinase plasminogen activator, cyclin-dependent kinase inhibitor 2b, MutL homolog 1, 5-methylcytosine binding domain 4, polymerase δ, α-tubulin, syntaxin 18, Ras homolog D, actin related protein 2/3 complex and cofilin. Via the analysis of Gene Ontology and pathways, these proteins in IPEC-J2 cells were related with some physiological functions, such as protein metabolism, glycolysis, cell proliferation, apoptosis and phagocytosis. Thus, these results suggest that leucine affects gut barrier function possibly via regulating cell proliferation and apoptosis, metabolism and phagocytosis.
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spelling pubmed-61163252018-08-31 Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis Mao, Xiangbing Ren, Man Chen, Daiwen Yu, Bing Che, Lianqiang He, Jun Luo, Junqiu Luo, Yuheng Wang, Jianping Sun, Hui Anim Nutr Molecular Nutrition Leucine can affect intestinal protein expressions, and improve mucosal immune function. However, little study has been conducted to determine the change of protein component by leucine treatment in intestine epithelial cells. The present study was to cover the key proteins and cell pathways that could be regulated by leucine treatment in porcine intestinal epithelial cell line (IPEC-J2) cells with the approach of proteome analysis. A total number of 3,211 proteins were identified in our approach by searching the database of Uniprot sus scrofa. Among identified proteins, there were 101 proteins expressed differently between control group and leucine group. Compared with the control group, there were 50 up-regulated proteins and 51 down-regulated proteins in leucine group. In these proteins, leucine treatment decreased the expression of some proteins including pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase, E3 ubiquitin ligase, cathepsin D, caspase 3 and caspase 6, and increased the levels of some proteins, such as some eukaryotic translation initiation factors, ubiquitin carboxyl-terminal hydrolase, DNA-related RNA polymerase II, urokinase plasminogen activator, cyclin-dependent kinase inhibitor 2b, MutL homolog 1, 5-methylcytosine binding domain 4, polymerase δ, α-tubulin, syntaxin 18, Ras homolog D, actin related protein 2/3 complex and cofilin. Via the analysis of Gene Ontology and pathways, these proteins in IPEC-J2 cells were related with some physiological functions, such as protein metabolism, glycolysis, cell proliferation, apoptosis and phagocytosis. Thus, these results suggest that leucine affects gut barrier function possibly via regulating cell proliferation and apoptosis, metabolism and phagocytosis. KeAi Publishing 2018-09 2018-04-05 /pmc/articles/PMC6116325/ /pubmed/30175261 http://dx.doi.org/10.1016/j.aninu.2018.03.006 Text en © 2018 Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Molecular Nutrition
Mao, Xiangbing
Ren, Man
Chen, Daiwen
Yu, Bing
Che, Lianqiang
He, Jun
Luo, Junqiu
Luo, Yuheng
Wang, Jianping
Sun, Hui
Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis
title Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis
title_full Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis
title_fullStr Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis
title_full_unstemmed Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis
title_short Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis
title_sort leucine modulates the ipec-j2 cell proteome associated with cell proliferation, metabolism and phagocytosis
topic Molecular Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116325/
https://www.ncbi.nlm.nih.gov/pubmed/30175261
http://dx.doi.org/10.1016/j.aninu.2018.03.006
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