Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19

It has previously been reported that human adipose-derived stem cells (hASCs) can promote the regeneration of damaged tissues in rats with liver failure through a ‘paracrine effect’. Here we demonstrate a therapeutic effect of hASCs derived Extracellular Vesicles (EVs) on rat models with acute liver...

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Autores principales: Jin, Yinpeng, Wang, Junyi, Li, Hongchao, Gao, Shane, Shi, Rongfeng, Yang, Danjing, Wang, Xianli, Wang, Xi, Zhu, Liang, Wang, Xiaojin, Chen, Chengwei, Ning, Ke, Fu, Qingchun, Xu, Jun, Gao, Zhengliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116414/
https://www.ncbi.nlm.nih.gov/pubmed/30077720
http://dx.doi.org/10.1016/j.ebiom.2018.07.015
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author Jin, Yinpeng
Wang, Junyi
Li, Hongchao
Gao, Shane
Shi, Rongfeng
Yang, Danjing
Wang, Xianli
Wang, Xi
Zhu, Liang
Wang, Xiaojin
Chen, Chengwei
Ning, Ke
Fu, Qingchun
Xu, Jun
Gao, Zhengliang
author_facet Jin, Yinpeng
Wang, Junyi
Li, Hongchao
Gao, Shane
Shi, Rongfeng
Yang, Danjing
Wang, Xianli
Wang, Xi
Zhu, Liang
Wang, Xiaojin
Chen, Chengwei
Ning, Ke
Fu, Qingchun
Xu, Jun
Gao, Zhengliang
author_sort Jin, Yinpeng
collection PubMed
description It has previously been reported that human adipose-derived stem cells (hASCs) can promote the regeneration of damaged tissues in rats with liver failure through a ‘paracrine effect’. Here we demonstrate a therapeutic effect of hASCs derived Extracellular Vesicles (EVs) on rat models with acute liver failure, as shown by the improvement of the survival rate by >70% compared to controls. Gene sequencing of rat liver revealed an increase in human long-chain non-coding RNA (lncRNA) H19 after hASC-derived EVs transplantation. When the H19 coding sequence was silenced in hASCs and EVs were then collected for treatment of rats with liver failure, we saw a decrease in the survival rate to 40%, compared to treatment with EVs generated from non-silenced hASCs. These data indicate that lncRNA H19 may be a potential therapeutic target for the treatment of liver failure.
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spelling pubmed-61164142018-08-31 Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19 Jin, Yinpeng Wang, Junyi Li, Hongchao Gao, Shane Shi, Rongfeng Yang, Danjing Wang, Xianli Wang, Xi Zhu, Liang Wang, Xiaojin Chen, Chengwei Ning, Ke Fu, Qingchun Xu, Jun Gao, Zhengliang EBioMedicine Research Paper It has previously been reported that human adipose-derived stem cells (hASCs) can promote the regeneration of damaged tissues in rats with liver failure through a ‘paracrine effect’. Here we demonstrate a therapeutic effect of hASCs derived Extracellular Vesicles (EVs) on rat models with acute liver failure, as shown by the improvement of the survival rate by >70% compared to controls. Gene sequencing of rat liver revealed an increase in human long-chain non-coding RNA (lncRNA) H19 after hASC-derived EVs transplantation. When the H19 coding sequence was silenced in hASCs and EVs were then collected for treatment of rats with liver failure, we saw a decrease in the survival rate to 40%, compared to treatment with EVs generated from non-silenced hASCs. These data indicate that lncRNA H19 may be a potential therapeutic target for the treatment of liver failure. Elsevier 2018-08-02 /pmc/articles/PMC6116414/ /pubmed/30077720 http://dx.doi.org/10.1016/j.ebiom.2018.07.015 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Jin, Yinpeng
Wang, Junyi
Li, Hongchao
Gao, Shane
Shi, Rongfeng
Yang, Danjing
Wang, Xianli
Wang, Xi
Zhu, Liang
Wang, Xiaojin
Chen, Chengwei
Ning, Ke
Fu, Qingchun
Xu, Jun
Gao, Zhengliang
Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19
title Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19
title_full Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19
title_fullStr Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19
title_full_unstemmed Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19
title_short Extracellular Vesicles Secreted by Human Adipose-derived Stem Cells (hASCs) Improve Survival Rate of Rats with Acute Liver Failure by Releasing lncRNA H19
title_sort extracellular vesicles secreted by human adipose-derived stem cells (hascs) improve survival rate of rats with acute liver failure by releasing lncrna h19
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116414/
https://www.ncbi.nlm.nih.gov/pubmed/30077720
http://dx.doi.org/10.1016/j.ebiom.2018.07.015
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