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Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage
BACKGROUND: Hypervirulent Klebsiella pneumoniae (hvKP) is emerging around the Asian-Pacific region and it is the major cause of the community-acquired pyogenic liver abscesses. Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) isolates were reported in France, China and Taiwan. Howe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116568/ https://www.ncbi.nlm.nih.gov/pubmed/30157774 http://dx.doi.org/10.1186/s12866-018-1236-2 |
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author | Shi, Qiucheng Lan, Peng Huang, Danyan Hua, Xiaoting Jiang, Yan Zhou, Jiancang Yu, Yunsong |
author_facet | Shi, Qiucheng Lan, Peng Huang, Danyan Hua, Xiaoting Jiang, Yan Zhou, Jiancang Yu, Yunsong |
author_sort | Shi, Qiucheng |
collection | PubMed |
description | BACKGROUND: Hypervirulent Klebsiella pneumoniae (hvKP) is emerging around the Asian-Pacific region and it is the major cause of the community-acquired pyogenic liver abscesses. Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) isolates were reported in France, China and Taiwan. However, the international-ally agreed definition for hvKP and the virulence level of hvKP are not clear. RESULTS: In this study, 56 hvKP isolates were collected from March 2008 to June 2012 and investigated by string test, capsule serotyping, multilocus sequence typing (MLST), virulence gene detection and serum resistance assay. Among the 56 K. pneumoniae isolates, 64.3% had the hypermucoviscosity phenotype, meanwhile, 64.3% were the K1 serotype and 19.6% were the K2 serotype. Within the K1 serotype, 94.4% were ST23, and within the K2 serotype, ST65, ST86 and ST375 accounted for the same percentage 27.3%. The serum resistance showed statistically normal distribution. According to the 50% lethal dose of Galleria. mellonella infection model, hvKP isolates were divided into high virulence level group and moderate virulence level group. The ability of each method evaluating the virulence level of hvKP was assessed using the area under the receiver operating characteristic curve. CONCLUSIONS: K1 ST23 K. pneumoniae was the most prevalent clone of the hvKP. However, K1 ST23 K. pneumoniae was the dominant clone in the moderate virulence level group. MLST was a relatively reliable evaluation method to discriminate the virulence level of hvKP in our study. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1236-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6116568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61165682018-10-02 Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage Shi, Qiucheng Lan, Peng Huang, Danyan Hua, Xiaoting Jiang, Yan Zhou, Jiancang Yu, Yunsong BMC Microbiol Research Article BACKGROUND: Hypervirulent Klebsiella pneumoniae (hvKP) is emerging around the Asian-Pacific region and it is the major cause of the community-acquired pyogenic liver abscesses. Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) isolates were reported in France, China and Taiwan. However, the international-ally agreed definition for hvKP and the virulence level of hvKP are not clear. RESULTS: In this study, 56 hvKP isolates were collected from March 2008 to June 2012 and investigated by string test, capsule serotyping, multilocus sequence typing (MLST), virulence gene detection and serum resistance assay. Among the 56 K. pneumoniae isolates, 64.3% had the hypermucoviscosity phenotype, meanwhile, 64.3% were the K1 serotype and 19.6% were the K2 serotype. Within the K1 serotype, 94.4% were ST23, and within the K2 serotype, ST65, ST86 and ST375 accounted for the same percentage 27.3%. The serum resistance showed statistically normal distribution. According to the 50% lethal dose of Galleria. mellonella infection model, hvKP isolates were divided into high virulence level group and moderate virulence level group. The ability of each method evaluating the virulence level of hvKP was assessed using the area under the receiver operating characteristic curve. CONCLUSIONS: K1 ST23 K. pneumoniae was the most prevalent clone of the hvKP. However, K1 ST23 K. pneumoniae was the dominant clone in the moderate virulence level group. MLST was a relatively reliable evaluation method to discriminate the virulence level of hvKP in our study. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1236-2) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-29 /pmc/articles/PMC6116568/ /pubmed/30157774 http://dx.doi.org/10.1186/s12866-018-1236-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Shi, Qiucheng Lan, Peng Huang, Danyan Hua, Xiaoting Jiang, Yan Zhou, Jiancang Yu, Yunsong Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage |
title | Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage |
title_full | Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage |
title_fullStr | Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage |
title_full_unstemmed | Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage |
title_short | Diversity of virulence level phenotype of hypervirulent Klebsiella pneumoniae from different sequence type lineage |
title_sort | diversity of virulence level phenotype of hypervirulent klebsiella pneumoniae from different sequence type lineage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116568/ https://www.ncbi.nlm.nih.gov/pubmed/30157774 http://dx.doi.org/10.1186/s12866-018-1236-2 |
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