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Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice

A single-injection vaccine formulation that provides for both a prime and a boost immunization would have various advantages over a multiple-injection regime. For such a vaccine formulation, it is essential that the booster dose is released after a certain, preferably adjustable, lag time. In this s...

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Autores principales: Amssoms, Katie, Born, Philip A., Beugeling, Max, De Clerck, Ben, Van Gulck, Ellen, Hinrichs, Wouter L. J., Frijlink, Henderik W., Grasmeijer, Niels, Kraus, Guenter, Sutmuller, Roger, Simmen, Kenny, Baert, Lieven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117011/
https://www.ncbi.nlm.nih.gov/pubmed/30161264
http://dx.doi.org/10.1371/journal.pone.0202961
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author Amssoms, Katie
Born, Philip A.
Beugeling, Max
De Clerck, Ben
Van Gulck, Ellen
Hinrichs, Wouter L. J.
Frijlink, Henderik W.
Grasmeijer, Niels
Kraus, Guenter
Sutmuller, Roger
Simmen, Kenny
Baert, Lieven
author_facet Amssoms, Katie
Born, Philip A.
Beugeling, Max
De Clerck, Ben
Van Gulck, Ellen
Hinrichs, Wouter L. J.
Frijlink, Henderik W.
Grasmeijer, Niels
Kraus, Guenter
Sutmuller, Roger
Simmen, Kenny
Baert, Lieven
author_sort Amssoms, Katie
collection PubMed
description A single-injection vaccine formulation that provides for both a prime and a boost immunization would have various advantages over a multiple-injection regime. For such a vaccine formulation, it is essential that the booster dose is released after a certain, preferably adjustable, lag time. In this study we investigated whether a core-shell based implant, containing ovalbumin as core material and poly(DL-lactic-co-glycolic acid) of various monomer ratios as shell material can be used to obtain such a booster release. An in vitro release study showed that the lag time after which the ovalbumin was released from the core-shell implant increased with increasing lactic to glycolic acid ratio of the polymer and ranged from 3–6 weeks. Fluorescence spectroscopy showed minimal differences between native ovalbumin and ovalbumin from core-shell implants that were incubated until just before the observed in vitro release. In addition, mice immunized with a subcutaneous inserted core-shell implant containing ovalbumin showed an ovalbumin-specific IgG1 antibody response after a lag time of 4 or 6–8 weeks. Moreover, delayed release of ovalbumin caused higher IgG1 antibody titers than conventional subcutaneous vaccination with ovalbumin dissolved in PBS. Collectively, these findings could contribute to the further development of a single-injection vaccine, making multiple injections of the vaccine superfluous.
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spelling pubmed-61170112018-09-16 Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice Amssoms, Katie Born, Philip A. Beugeling, Max De Clerck, Ben Van Gulck, Ellen Hinrichs, Wouter L. J. Frijlink, Henderik W. Grasmeijer, Niels Kraus, Guenter Sutmuller, Roger Simmen, Kenny Baert, Lieven PLoS One Research Article A single-injection vaccine formulation that provides for both a prime and a boost immunization would have various advantages over a multiple-injection regime. For such a vaccine formulation, it is essential that the booster dose is released after a certain, preferably adjustable, lag time. In this study we investigated whether a core-shell based implant, containing ovalbumin as core material and poly(DL-lactic-co-glycolic acid) of various monomer ratios as shell material can be used to obtain such a booster release. An in vitro release study showed that the lag time after which the ovalbumin was released from the core-shell implant increased with increasing lactic to glycolic acid ratio of the polymer and ranged from 3–6 weeks. Fluorescence spectroscopy showed minimal differences between native ovalbumin and ovalbumin from core-shell implants that were incubated until just before the observed in vitro release. In addition, mice immunized with a subcutaneous inserted core-shell implant containing ovalbumin showed an ovalbumin-specific IgG1 antibody response after a lag time of 4 or 6–8 weeks. Moreover, delayed release of ovalbumin caused higher IgG1 antibody titers than conventional subcutaneous vaccination with ovalbumin dissolved in PBS. Collectively, these findings could contribute to the further development of a single-injection vaccine, making multiple injections of the vaccine superfluous. Public Library of Science 2018-08-30 /pmc/articles/PMC6117011/ /pubmed/30161264 http://dx.doi.org/10.1371/journal.pone.0202961 Text en © 2018 Amssoms et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Amssoms, Katie
Born, Philip A.
Beugeling, Max
De Clerck, Ben
Van Gulck, Ellen
Hinrichs, Wouter L. J.
Frijlink, Henderik W.
Grasmeijer, Niels
Kraus, Guenter
Sutmuller, Roger
Simmen, Kenny
Baert, Lieven
Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
title Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
title_full Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
title_fullStr Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
title_full_unstemmed Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
title_short Ovalbumin-containing core-shell implants suitable to obtain a delayed IgG1 antibody response in support of a biphasic pulsatile release profile in mice
title_sort ovalbumin-containing core-shell implants suitable to obtain a delayed igg1 antibody response in support of a biphasic pulsatile release profile in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117011/
https://www.ncbi.nlm.nih.gov/pubmed/30161264
http://dx.doi.org/10.1371/journal.pone.0202961
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