The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli

The Congo red derivative (E,E)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (FSB) specifically stains the functional amyloid curli in Escherichia coli biofilms. FSB binds to curli with similar affinity as Congo red, yet exhibits much greater fluorescence upon binding to curli as compa...

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Detalles Bibliográficos
Autores principales: Reichhardt, Courtney, Cegelski, Lynette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117054/
https://www.ncbi.nlm.nih.gov/pubmed/30161215
http://dx.doi.org/10.1371/journal.pone.0203226
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author Reichhardt, Courtney
Cegelski, Lynette
author_facet Reichhardt, Courtney
Cegelski, Lynette
author_sort Reichhardt, Courtney
collection PubMed
description The Congo red derivative (E,E)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (FSB) specifically stains the functional amyloid curli in Escherichia coli biofilms. FSB binds to curli with similar affinity as Congo red, yet exhibits much greater fluorescence upon binding to curli as compared to Congo red and does not exhibit undesired binding to the cellulosic component of the biofilm. Thus, FSB presents a powerful tool to identify and visualize curli in E. coli biofilms and also enables new biophysical investigations of curli.
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spelling pubmed-61170542018-09-16 The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli Reichhardt, Courtney Cegelski, Lynette PLoS One Research Article The Congo red derivative (E,E)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (FSB) specifically stains the functional amyloid curli in Escherichia coli biofilms. FSB binds to curli with similar affinity as Congo red, yet exhibits much greater fluorescence upon binding to curli as compared to Congo red and does not exhibit undesired binding to the cellulosic component of the biofilm. Thus, FSB presents a powerful tool to identify and visualize curli in E. coli biofilms and also enables new biophysical investigations of curli. Public Library of Science 2018-08-30 /pmc/articles/PMC6117054/ /pubmed/30161215 http://dx.doi.org/10.1371/journal.pone.0203226 Text en © 2018 Reichhardt, Cegelski http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Reichhardt, Courtney
Cegelski, Lynette
The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli
title The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli
title_full The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli
title_fullStr The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli
title_full_unstemmed The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli
title_short The Congo red derivative FSB binds to curli amyloid fibers and specifically stains curliated E. coli
title_sort congo red derivative fsb binds to curli amyloid fibers and specifically stains curliated e. coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117054/
https://www.ncbi.nlm.nih.gov/pubmed/30161215
http://dx.doi.org/10.1371/journal.pone.0203226
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