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Characterization of mRNA polyadenylation in the apicomplexa

Messenger RNA polyadenylation is a universal aspect of gene expression in eukaryotes. In well-established model organisms, this process is mediated by a conserved complex of 15–20 subunits. To better understand this process in apicomplexans, a group of unicellular parasites that causes serious disea...

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Autores principales: Stevens, Ashley T., Howe, Daniel K., Hunt, Arthur G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117058/
https://www.ncbi.nlm.nih.gov/pubmed/30161237
http://dx.doi.org/10.1371/journal.pone.0203317
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author Stevens, Ashley T.
Howe, Daniel K.
Hunt, Arthur G.
author_facet Stevens, Ashley T.
Howe, Daniel K.
Hunt, Arthur G.
author_sort Stevens, Ashley T.
collection PubMed
description Messenger RNA polyadenylation is a universal aspect of gene expression in eukaryotes. In well-established model organisms, this process is mediated by a conserved complex of 15–20 subunits. To better understand this process in apicomplexans, a group of unicellular parasites that causes serious disease in humans and livestock, a computational and high throughput sequencing study of the polyadenylation complex and poly(A) sites in several species was conducted. BLAST-based searches for orthologs of the human polyadenylation complex yielded clear matches to only two—poly(A) polymerase and CPSF73—of the 19 proteins used as queries in this analysis. As the human subunits that recognize the AAUAAA polyadenylation signal (PAS) were not immediately obvious, a computational analysis of sequences adjacent to experimentally-determined apicomplexan poly(A) sites was conducted. The results of this study showed that there exists in apicomplexans an A-rich region that corresponds in position to the AAUAAA PAS. The set of experimentally-determined sites in one species, Sarcocystis neurona, was further analyzed to evaluate the extent and significance of alternative poly(A) site choice in this organism. The results showed that almost 80% of S. neurona genes possess more than one poly(A) site, and that more than 780 sites showed differential usage in the two developmental stages–extracellular merozoites and intracellular schizonts–studied. These sites affected more than 450 genes, and included a disproportionate number of genes that encode membrane transporters and ribosomal proteins. Taken together, these results reveal that apicomplexan species seem to possess a poly(A) signal analogous to AAUAAA even though genes that may encode obvious counterparts of the AAUAAA-recognizing proteins are absent in these organisms. They also indicate that, as is the case in other eukaryotes, alternative polyadenylation is a widespread phenomenon in S. neurona that has the potential to impact growth and development.
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spelling pubmed-61170582018-09-16 Characterization of mRNA polyadenylation in the apicomplexa Stevens, Ashley T. Howe, Daniel K. Hunt, Arthur G. PLoS One Research Article Messenger RNA polyadenylation is a universal aspect of gene expression in eukaryotes. In well-established model organisms, this process is mediated by a conserved complex of 15–20 subunits. To better understand this process in apicomplexans, a group of unicellular parasites that causes serious disease in humans and livestock, a computational and high throughput sequencing study of the polyadenylation complex and poly(A) sites in several species was conducted. BLAST-based searches for orthologs of the human polyadenylation complex yielded clear matches to only two—poly(A) polymerase and CPSF73—of the 19 proteins used as queries in this analysis. As the human subunits that recognize the AAUAAA polyadenylation signal (PAS) were not immediately obvious, a computational analysis of sequences adjacent to experimentally-determined apicomplexan poly(A) sites was conducted. The results of this study showed that there exists in apicomplexans an A-rich region that corresponds in position to the AAUAAA PAS. The set of experimentally-determined sites in one species, Sarcocystis neurona, was further analyzed to evaluate the extent and significance of alternative poly(A) site choice in this organism. The results showed that almost 80% of S. neurona genes possess more than one poly(A) site, and that more than 780 sites showed differential usage in the two developmental stages–extracellular merozoites and intracellular schizonts–studied. These sites affected more than 450 genes, and included a disproportionate number of genes that encode membrane transporters and ribosomal proteins. Taken together, these results reveal that apicomplexan species seem to possess a poly(A) signal analogous to AAUAAA even though genes that may encode obvious counterparts of the AAUAAA-recognizing proteins are absent in these organisms. They also indicate that, as is the case in other eukaryotes, alternative polyadenylation is a widespread phenomenon in S. neurona that has the potential to impact growth and development. Public Library of Science 2018-08-30 /pmc/articles/PMC6117058/ /pubmed/30161237 http://dx.doi.org/10.1371/journal.pone.0203317 Text en © 2018 Stevens et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Stevens, Ashley T.
Howe, Daniel K.
Hunt, Arthur G.
Characterization of mRNA polyadenylation in the apicomplexa
title Characterization of mRNA polyadenylation in the apicomplexa
title_full Characterization of mRNA polyadenylation in the apicomplexa
title_fullStr Characterization of mRNA polyadenylation in the apicomplexa
title_full_unstemmed Characterization of mRNA polyadenylation in the apicomplexa
title_short Characterization of mRNA polyadenylation in the apicomplexa
title_sort characterization of mrna polyadenylation in the apicomplexa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117058/
https://www.ncbi.nlm.nih.gov/pubmed/30161237
http://dx.doi.org/10.1371/journal.pone.0203317
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