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Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern
Although Helicobacter pylori (H. pylori) is a highly significant pathogen, its source remains unclear. Many people consume chicken daily as a source of animal protein worldwide; thus, hygienic methods of supplying chickens for consumption are critical for public health. Therefore, our study examined...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117242/ https://www.ncbi.nlm.nih.gov/pubmed/30174504 http://dx.doi.org/10.1016/j.sjbs.2018.02.002 |
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author | Hamada, Mohamed Elbehiry, Ayman Marzouk, Eman Moussa, Ihab M. Hessain, Ashgan Mohamed Alhaji, Jwaher Haji Heme, Hassan A. Zahran, Rasha Abdeen, Eman |
author_facet | Hamada, Mohamed Elbehiry, Ayman Marzouk, Eman Moussa, Ihab M. Hessain, Ashgan Mohamed Alhaji, Jwaher Haji Heme, Hassan A. Zahran, Rasha Abdeen, Eman |
author_sort | Hamada, Mohamed |
collection | PubMed |
description | Although Helicobacter pylori (H. pylori) is a highly significant pathogen, its source remains unclear. Many people consume chicken daily as a source of animal protein worldwide; thus, hygienic methods of supplying chickens for consumption are critical for public health. Therefore, our study examined the distribution of the glmM (ureC), babA2, vacA and cagA virulence genes in H. pylori strains in chicken meat and giblets (gizzards and livers) and the resistance of the strains to various antibiotics. Ninety chicken meat, gizzard and liver samples were obtained from a semi-automatic abattoir in Sadat City, Egypt, and were cultured and preliminarily analyzed using biochemical tests. The presence of the ureC, babA2, vacA and cagA genotypes was tested for in samples positive for H. pylori by multiplex polymerase chain reaction (Multiplex-PCR). The resistance of H. pylori to various antimicrobial drugs was tested using the disc diffusion method. In total, 7 of the 90 chicken samples were positive for H. pylori (7.78%); in 3/7 (42.85%) samples, the bacteria were found in the chicken liver, while the bacteria were found in the meat in 2/7 (28.57%) and in the gizzard in 2/7 (28.57%) samples. The total prevalence of both the ureC and babA2 genes in the isolated H. pylori strains was 100%, while the prevalence of the vacA and cagA genes was 57.1% and 42.9%, respectively. The resistance of H. pylori to the antibiotics utilized in our study was 100% for streptomycin; 85.7% for amoxicillin and penicillin; 71.4% for oxytetracycline, nalidixic acid and ampicillin; 57.1% for sulfamethoxazole and erythromycin; and 42.9% for neomycin, chloramphenicol and norfloxacin. In conclusion, the chicken meat and giblets were tainted by H. pylori, with a higher occurrence of the ureC, babA2, vacA and cagA genotypes. Future investigations should investigate the resistance of H. pylori to various antimicrobial agents in Egypt. |
format | Online Article Text |
id | pubmed-6117242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61172422018-08-31 Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern Hamada, Mohamed Elbehiry, Ayman Marzouk, Eman Moussa, Ihab M. Hessain, Ashgan Mohamed Alhaji, Jwaher Haji Heme, Hassan A. Zahran, Rasha Abdeen, Eman Saudi J Biol Sci Article Although Helicobacter pylori (H. pylori) is a highly significant pathogen, its source remains unclear. Many people consume chicken daily as a source of animal protein worldwide; thus, hygienic methods of supplying chickens for consumption are critical for public health. Therefore, our study examined the distribution of the glmM (ureC), babA2, vacA and cagA virulence genes in H. pylori strains in chicken meat and giblets (gizzards and livers) and the resistance of the strains to various antibiotics. Ninety chicken meat, gizzard and liver samples were obtained from a semi-automatic abattoir in Sadat City, Egypt, and were cultured and preliminarily analyzed using biochemical tests. The presence of the ureC, babA2, vacA and cagA genotypes was tested for in samples positive for H. pylori by multiplex polymerase chain reaction (Multiplex-PCR). The resistance of H. pylori to various antimicrobial drugs was tested using the disc diffusion method. In total, 7 of the 90 chicken samples were positive for H. pylori (7.78%); in 3/7 (42.85%) samples, the bacteria were found in the chicken liver, while the bacteria were found in the meat in 2/7 (28.57%) and in the gizzard in 2/7 (28.57%) samples. The total prevalence of both the ureC and babA2 genes in the isolated H. pylori strains was 100%, while the prevalence of the vacA and cagA genes was 57.1% and 42.9%, respectively. The resistance of H. pylori to the antibiotics utilized in our study was 100% for streptomycin; 85.7% for amoxicillin and penicillin; 71.4% for oxytetracycline, nalidixic acid and ampicillin; 57.1% for sulfamethoxazole and erythromycin; and 42.9% for neomycin, chloramphenicol and norfloxacin. In conclusion, the chicken meat and giblets were tainted by H. pylori, with a higher occurrence of the ureC, babA2, vacA and cagA genotypes. Future investigations should investigate the resistance of H. pylori to various antimicrobial agents in Egypt. Elsevier 2018-09 2018-02-09 /pmc/articles/PMC6117242/ /pubmed/30174504 http://dx.doi.org/10.1016/j.sjbs.2018.02.002 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Hamada, Mohamed Elbehiry, Ayman Marzouk, Eman Moussa, Ihab M. Hessain, Ashgan Mohamed Alhaji, Jwaher Haji Heme, Hassan A. Zahran, Rasha Abdeen, Eman Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern |
title | Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern |
title_full | Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern |
title_fullStr | Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern |
title_full_unstemmed | Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern |
title_short | Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern |
title_sort | helicobacter pylori in a poultry slaughterhouse: prevalence, genotyping and antibiotic resistance pattern |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117242/ https://www.ncbi.nlm.nih.gov/pubmed/30174504 http://dx.doi.org/10.1016/j.sjbs.2018.02.002 |
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