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A myosin II nanomachine mimicking the striated muscle

The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. H...

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Autores principales: Pertici, Irene, Bongini, Lorenzo, Melli, Luca, Bianchi, Giulio, Salvi, Luca, Falorsi, Giulia, Squarci, Caterina, Bozó, Tamás, Cojoc, Dan, Kellermayer, Miklós S. Z., Lombardi, Vincenzo, Bianco, Pasquale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117265/
https://www.ncbi.nlm.nih.gov/pubmed/30166542
http://dx.doi.org/10.1038/s41467-018-06073-9
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author Pertici, Irene
Bongini, Lorenzo
Melli, Luca
Bianchi, Giulio
Salvi, Luca
Falorsi, Giulia
Squarci, Caterina
Bozó, Tamás
Cojoc, Dan
Kellermayer, Miklós S. Z.
Lombardi, Vincenzo
Bianco, Pasquale
author_facet Pertici, Irene
Bongini, Lorenzo
Melli, Luca
Bianchi, Giulio
Salvi, Luca
Falorsi, Giulia
Squarci, Caterina
Bozó, Tamás
Cojoc, Dan
Kellermayer, Miklós S. Z.
Lombardi, Vincenzo
Bianco, Pasquale
author_sort Pertici, Irene
collection PubMed
description The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal—and regulatory—protein environment.
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spelling pubmed-61172652018-09-04 A myosin II nanomachine mimicking the striated muscle Pertici, Irene Bongini, Lorenzo Melli, Luca Bianchi, Giulio Salvi, Luca Falorsi, Giulia Squarci, Caterina Bozó, Tamás Cojoc, Dan Kellermayer, Miklós S. Z. Lombardi, Vincenzo Bianco, Pasquale Nat Commun Article The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal—and regulatory—protein environment. Nature Publishing Group UK 2018-08-30 /pmc/articles/PMC6117265/ /pubmed/30166542 http://dx.doi.org/10.1038/s41467-018-06073-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pertici, Irene
Bongini, Lorenzo
Melli, Luca
Bianchi, Giulio
Salvi, Luca
Falorsi, Giulia
Squarci, Caterina
Bozó, Tamás
Cojoc, Dan
Kellermayer, Miklós S. Z.
Lombardi, Vincenzo
Bianco, Pasquale
A myosin II nanomachine mimicking the striated muscle
title A myosin II nanomachine mimicking the striated muscle
title_full A myosin II nanomachine mimicking the striated muscle
title_fullStr A myosin II nanomachine mimicking the striated muscle
title_full_unstemmed A myosin II nanomachine mimicking the striated muscle
title_short A myosin II nanomachine mimicking the striated muscle
title_sort myosin ii nanomachine mimicking the striated muscle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117265/
https://www.ncbi.nlm.nih.gov/pubmed/30166542
http://dx.doi.org/10.1038/s41467-018-06073-9
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