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Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications

The high prevalence of bone defects has become a worldwide problem. Despite the significant amount of research on the subject, the available therapeutic solutions lack efficiency. Autografts, the most commonly used approaches to treat bone defects, have limitations such as donor site morbidity, pain...

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Autores principales: Diogo, Gabriela S., López-Senra, Estefânia, Pirraco, Rogério P., Canadas, Raphael F., Fernandes, Emanuel M., Serra, Julia, Pérez-Martín, Ricardo I., Sotelo, Carmen G., Marques, Alexandra P., González, Pio, Moreira-Silva, Joana, Silva, Tiago H., Reis, Rui L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117652/
https://www.ncbi.nlm.nih.gov/pubmed/30081528
http://dx.doi.org/10.3390/md16080269
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author Diogo, Gabriela S.
López-Senra, Estefânia
Pirraco, Rogério P.
Canadas, Raphael F.
Fernandes, Emanuel M.
Serra, Julia
Pérez-Martín, Ricardo I.
Sotelo, Carmen G.
Marques, Alexandra P.
González, Pio
Moreira-Silva, Joana
Silva, Tiago H.
Reis, Rui L.
author_facet Diogo, Gabriela S.
López-Senra, Estefânia
Pirraco, Rogério P.
Canadas, Raphael F.
Fernandes, Emanuel M.
Serra, Julia
Pérez-Martín, Ricardo I.
Sotelo, Carmen G.
Marques, Alexandra P.
González, Pio
Moreira-Silva, Joana
Silva, Tiago H.
Reis, Rui L.
author_sort Diogo, Gabriela S.
collection PubMed
description The high prevalence of bone defects has become a worldwide problem. Despite the significant amount of research on the subject, the available therapeutic solutions lack efficiency. Autografts, the most commonly used approaches to treat bone defects, have limitations such as donor site morbidity, pain and lack of donor site. Marine resources emerge as an attractive alternative to extract bioactive compounds for further use in bone tissue-engineering approaches. On one hand they can be isolated from by-products, at low cost, creating value from products that are considered waste for the fish transformation industry. One the other hand, religious constraints will be avoided. We isolated two marine origin materials, collagen from shark skin (Prionace glauca) and calcium phosphates from the teeth of two different shark species (Prionace glauca and Isurus oxyrinchus), and further proposed to mix them to produce 3D composite structures for hard tissue applications. Two crosslinking agents, 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride/N-Hydroxysuccinimide (EDC/NHS) and hexamethylene diisocyanate (HMDI), were tested to enhance the scaffolds’ properties, with EDC/NHS resulting in better properties. The characterization of the structures showed that the developed composites could support attachment and proliferation of osteoblast-like cells. A promising scaffold for the engineering of bone tissue is thus proposed, based on a strategy of marine by-products valorisation.
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spelling pubmed-61176522018-09-05 Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications Diogo, Gabriela S. López-Senra, Estefânia Pirraco, Rogério P. Canadas, Raphael F. Fernandes, Emanuel M. Serra, Julia Pérez-Martín, Ricardo I. Sotelo, Carmen G. Marques, Alexandra P. González, Pio Moreira-Silva, Joana Silva, Tiago H. Reis, Rui L. Mar Drugs Article The high prevalence of bone defects has become a worldwide problem. Despite the significant amount of research on the subject, the available therapeutic solutions lack efficiency. Autografts, the most commonly used approaches to treat bone defects, have limitations such as donor site morbidity, pain and lack of donor site. Marine resources emerge as an attractive alternative to extract bioactive compounds for further use in bone tissue-engineering approaches. On one hand they can be isolated from by-products, at low cost, creating value from products that are considered waste for the fish transformation industry. One the other hand, religious constraints will be avoided. We isolated two marine origin materials, collagen from shark skin (Prionace glauca) and calcium phosphates from the teeth of two different shark species (Prionace glauca and Isurus oxyrinchus), and further proposed to mix them to produce 3D composite structures for hard tissue applications. Two crosslinking agents, 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride/N-Hydroxysuccinimide (EDC/NHS) and hexamethylene diisocyanate (HMDI), were tested to enhance the scaffolds’ properties, with EDC/NHS resulting in better properties. The characterization of the structures showed that the developed composites could support attachment and proliferation of osteoblast-like cells. A promising scaffold for the engineering of bone tissue is thus proposed, based on a strategy of marine by-products valorisation. MDPI 2018-08-03 /pmc/articles/PMC6117652/ /pubmed/30081528 http://dx.doi.org/10.3390/md16080269 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Diogo, Gabriela S.
López-Senra, Estefânia
Pirraco, Rogério P.
Canadas, Raphael F.
Fernandes, Emanuel M.
Serra, Julia
Pérez-Martín, Ricardo I.
Sotelo, Carmen G.
Marques, Alexandra P.
González, Pio
Moreira-Silva, Joana
Silva, Tiago H.
Reis, Rui L.
Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications
title Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications
title_full Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications
title_fullStr Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications
title_full_unstemmed Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications
title_short Marine Collagen/Apatite Composite Scaffolds Envisaging Hard Tissue Applications
title_sort marine collagen/apatite composite scaffolds envisaging hard tissue applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117652/
https://www.ncbi.nlm.nih.gov/pubmed/30081528
http://dx.doi.org/10.3390/md16080269
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