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Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications

In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological...

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Autores principales: Tihan, Gratiela Teodora, Zgarian, Roxana Gabriela, Berteanu, Elena, Ionita, Daniela, Totea, Georgeta, Iordachel, Catalin, Tatia, Rodica, Prodana, Mariana, Demetrescu, Ioana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117669/
https://www.ncbi.nlm.nih.gov/pubmed/30126191
http://dx.doi.org/10.3390/md16080287
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author Tihan, Gratiela Teodora
Zgarian, Roxana Gabriela
Berteanu, Elena
Ionita, Daniela
Totea, Georgeta
Iordachel, Catalin
Tatia, Rodica
Prodana, Mariana
Demetrescu, Ioana
author_facet Tihan, Gratiela Teodora
Zgarian, Roxana Gabriela
Berteanu, Elena
Ionita, Daniela
Totea, Georgeta
Iordachel, Catalin
Tatia, Rodica
Prodana, Mariana
Demetrescu, Ioana
author_sort Tihan, Gratiela Teodora
collection PubMed
description In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg(2+) on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg(2+) led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg(2+). The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.
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spelling pubmed-61176692018-09-05 Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications Tihan, Gratiela Teodora Zgarian, Roxana Gabriela Berteanu, Elena Ionita, Daniela Totea, Georgeta Iordachel, Catalin Tatia, Rodica Prodana, Mariana Demetrescu, Ioana Mar Drugs Article In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg(2+) on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg(2+) led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg(2+). The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions. MDPI 2018-08-18 /pmc/articles/PMC6117669/ /pubmed/30126191 http://dx.doi.org/10.3390/md16080287 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tihan, Gratiela Teodora
Zgarian, Roxana Gabriela
Berteanu, Elena
Ionita, Daniela
Totea, Georgeta
Iordachel, Catalin
Tatia, Rodica
Prodana, Mariana
Demetrescu, Ioana
Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications
title Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications
title_full Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications
title_fullStr Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications
title_full_unstemmed Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications
title_short Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg(2+) for Biomedical Applications
title_sort alkaline phosphatase immobilization on new chitosan membranes with mg(2+) for biomedical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117669/
https://www.ncbi.nlm.nih.gov/pubmed/30126191
http://dx.doi.org/10.3390/md16080287
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