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Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

The primary goal of this work was to investigate the resulting morphology of a mammalian cell deposited on three-dimensional nanocomposites constructed of tantalum and silicon oxide. Vero cells were used as a model. The nanocomposite materials contained comb structures with equal-width trenches and...

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Autores principales: Moussa, Hassan I., Logan, Megan, Chan, Wing Y., Wong, Kingsley, Rao, Zheng, Aucoin, Marc G., Tsui, Ting Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117680/
https://www.ncbi.nlm.nih.gov/pubmed/30060574
http://dx.doi.org/10.3390/ma11081306
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author Moussa, Hassan I.
Logan, Megan
Chan, Wing Y.
Wong, Kingsley
Rao, Zheng
Aucoin, Marc G.
Tsui, Ting Y.
author_facet Moussa, Hassan I.
Logan, Megan
Chan, Wing Y.
Wong, Kingsley
Rao, Zheng
Aucoin, Marc G.
Tsui, Ting Y.
author_sort Moussa, Hassan I.
collection PubMed
description The primary goal of this work was to investigate the resulting morphology of a mammalian cell deposited on three-dimensional nanocomposites constructed of tantalum and silicon oxide. Vero cells were used as a model. The nanocomposite materials contained comb structures with equal-width trenches and lines. High-resolution scanning electron microscopy and fluorescence microscopy were used to image the alignment and elongation of cells. Cells were sensitive to the trench widths, and their observed behavior could be separated into three different regimes corresponding to different spreading mechanism. Cells on fine structures (trench widths of 0.21 to 0.5 μm) formed bridges across trench openings. On larger trenches (from 1 to 10 μm), cells formed a conformal layer matching the surface topographical features. When the trenches were larger than 10 μm, the majority of cells spread like those on blanket tantalum films; however, a significant proportion adhered to the trench sidewalls or bottom corner junctions. Pseudopodia extending from the bulk of the cell were readily observed in this work and a minimum effective diameter of ~50 nm was determined for stable adhesion to a tantalum surface. This sized structure is consistent with the ability of pseudopodia to accommodate ~4–6 integrin molecules.
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spelling pubmed-61176802018-09-05 Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites Moussa, Hassan I. Logan, Megan Chan, Wing Y. Wong, Kingsley Rao, Zheng Aucoin, Marc G. Tsui, Ting Y. Materials (Basel) Article The primary goal of this work was to investigate the resulting morphology of a mammalian cell deposited on three-dimensional nanocomposites constructed of tantalum and silicon oxide. Vero cells were used as a model. The nanocomposite materials contained comb structures with equal-width trenches and lines. High-resolution scanning electron microscopy and fluorescence microscopy were used to image the alignment and elongation of cells. Cells were sensitive to the trench widths, and their observed behavior could be separated into three different regimes corresponding to different spreading mechanism. Cells on fine structures (trench widths of 0.21 to 0.5 μm) formed bridges across trench openings. On larger trenches (from 1 to 10 μm), cells formed a conformal layer matching the surface topographical features. When the trenches were larger than 10 μm, the majority of cells spread like those on blanket tantalum films; however, a significant proportion adhered to the trench sidewalls or bottom corner junctions. Pseudopodia extending from the bulk of the cell were readily observed in this work and a minimum effective diameter of ~50 nm was determined for stable adhesion to a tantalum surface. This sized structure is consistent with the ability of pseudopodia to accommodate ~4–6 integrin molecules. MDPI 2018-07-28 /pmc/articles/PMC6117680/ /pubmed/30060574 http://dx.doi.org/10.3390/ma11081306 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moussa, Hassan I.
Logan, Megan
Chan, Wing Y.
Wong, Kingsley
Rao, Zheng
Aucoin, Marc G.
Tsui, Ting Y.
Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites
title Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites
title_full Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites
title_fullStr Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites
title_full_unstemmed Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites
title_short Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites
title_sort pattern-dependent mammalian cell (vero) morphology on tantalum/silicon oxide 3d nanocomposites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117680/
https://www.ncbi.nlm.nih.gov/pubmed/30060574
http://dx.doi.org/10.3390/ma11081306
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