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Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon

BACKGROUND: Malaria vectors are increasingly developing resistance to insecticides across Africa. The impact of such resistance on the continued effectiveness of insecticide-based interventions remains unclear due to poor characterization of vector populations. This study reports the characterizatio...

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Autores principales: Menze, Benjamin D., Wondji, Murielle J., Tchapga, William, Tchoupo, Micareme, Riveron, Jacob M., Wondji, Charles S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117958/
https://www.ncbi.nlm.nih.gov/pubmed/30165863
http://dx.doi.org/10.1186/s12936-018-2467-2
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author Menze, Benjamin D.
Wondji, Murielle J.
Tchapga, William
Tchoupo, Micareme
Riveron, Jacob M.
Wondji, Charles S.
author_facet Menze, Benjamin D.
Wondji, Murielle J.
Tchapga, William
Tchoupo, Micareme
Riveron, Jacob M.
Wondji, Charles S.
author_sort Menze, Benjamin D.
collection PubMed
description BACKGROUND: Malaria vectors are increasingly developing resistance to insecticides across Africa. The impact of such resistance on the continued effectiveness of insecticide-based interventions remains unclear due to poor characterization of vector populations. This study reports the characterization of malaria vectors at Mibellon, a selected site in Cameroon for experimental hut study, including species composition, Plasmodium infection rate, resistance profiles and mechanisms. METHODS: Indoor resting blood-fed Anopheles mosquitoes were collected from houses at Mibellon in 2017 and forced to lay eggs to generate F(1) adult mosquitoes. Insecticides susceptibility bioassays were performed on the F(1) adult mosquitoes following the WHO protocol to assess resistance profile to insecticides. The molecular basis of resistance and Plasmodium infection rate were investigated using TaqMan genotyping. RESULTS: Anopheles funestus sensu stricto (s.s.) was predominant in Mibellon (80%) followed by Anopheles gambiae s.s. (20%). High levels of resistance to pyrethroids and organochlorides were observed for both species. Moderate resistance was observed against bendiocarb (carbamate) in both species, but relatively higher in An. gambiae s.s. In contrast, full susceptibility was recorded for the organophosphate malathion. The PBO synergist assays with permethrin and deltamethrin revealed a significant recovery of the susceptibility in Anopheles funestus s.s. population (48.8 to 98.1% mortality and 38.3 to 96.5% mortality, respectively). The DDT/pyrethroid 119F-GSTe2 resistant allele (28.1%) and the dieldrin 296S-RDL resistant (9.7%) were detected in An. funestus s.s. The high pyrethroid/DDT resistance in An. gambiae correlated with the high frequency of 1014F knockdown resistance allele (63.9%). The 1014S-kdr allele was detected at low frequency (1.97%). The Plasmodium infection rate was 20% in An. gambiae, whereas An. funestus exhibited an oocyst rate of 15 and 5% for the sporozoite rate. CONCLUSION: These results highlight the increasing spread of insecticide resistance and the challenges that control programmes face to maintain the continued effectiveness of insecticide-based interventions.
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spelling pubmed-61179582018-09-05 Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon Menze, Benjamin D. Wondji, Murielle J. Tchapga, William Tchoupo, Micareme Riveron, Jacob M. Wondji, Charles S. Malar J Research BACKGROUND: Malaria vectors are increasingly developing resistance to insecticides across Africa. The impact of such resistance on the continued effectiveness of insecticide-based interventions remains unclear due to poor characterization of vector populations. This study reports the characterization of malaria vectors at Mibellon, a selected site in Cameroon for experimental hut study, including species composition, Plasmodium infection rate, resistance profiles and mechanisms. METHODS: Indoor resting blood-fed Anopheles mosquitoes were collected from houses at Mibellon in 2017 and forced to lay eggs to generate F(1) adult mosquitoes. Insecticides susceptibility bioassays were performed on the F(1) adult mosquitoes following the WHO protocol to assess resistance profile to insecticides. The molecular basis of resistance and Plasmodium infection rate were investigated using TaqMan genotyping. RESULTS: Anopheles funestus sensu stricto (s.s.) was predominant in Mibellon (80%) followed by Anopheles gambiae s.s. (20%). High levels of resistance to pyrethroids and organochlorides were observed for both species. Moderate resistance was observed against bendiocarb (carbamate) in both species, but relatively higher in An. gambiae s.s. In contrast, full susceptibility was recorded for the organophosphate malathion. The PBO synergist assays with permethrin and deltamethrin revealed a significant recovery of the susceptibility in Anopheles funestus s.s. population (48.8 to 98.1% mortality and 38.3 to 96.5% mortality, respectively). The DDT/pyrethroid 119F-GSTe2 resistant allele (28.1%) and the dieldrin 296S-RDL resistant (9.7%) were detected in An. funestus s.s. The high pyrethroid/DDT resistance in An. gambiae correlated with the high frequency of 1014F knockdown resistance allele (63.9%). The 1014S-kdr allele was detected at low frequency (1.97%). The Plasmodium infection rate was 20% in An. gambiae, whereas An. funestus exhibited an oocyst rate of 15 and 5% for the sporozoite rate. CONCLUSION: These results highlight the increasing spread of insecticide resistance and the challenges that control programmes face to maintain the continued effectiveness of insecticide-based interventions. BioMed Central 2018-08-30 /pmc/articles/PMC6117958/ /pubmed/30165863 http://dx.doi.org/10.1186/s12936-018-2467-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Menze, Benjamin D.
Wondji, Murielle J.
Tchapga, William
Tchoupo, Micareme
Riveron, Jacob M.
Wondji, Charles S.
Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon
title Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon
title_full Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon
title_fullStr Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon
title_full_unstemmed Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon
title_short Bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central Cameroon
title_sort bionomics and insecticides resistance profiling of malaria vectors at a selected site for experimental hut trials in central cameroon
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117958/
https://www.ncbi.nlm.nih.gov/pubmed/30165863
http://dx.doi.org/10.1186/s12936-018-2467-2
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